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ESAM Antibody (MA5-24146) in ICC/IF
Immunocytochemical analysis of ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using mouse Anti-human ESAM Monoclonal Antibody (Product # MA5-24146) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (yello and counterstained with DAPI (blue).
Product Details
MA5-24146
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG2B
Class
Monoclonal
Type
Antibody
Clone
408519
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ESAM Gln30-Ala247
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606177
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2310008D05Rik; Endothelial cell-selective adhesion molecule; HUEL (C4orf1)-interacting protein; LP4791 protein
Gene Aliases: ESAM; UNQ220/PRO246; W117m
UniProt ID: (Human) Q96AP7
Entrez Gene ID: (Human) 90952
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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