Invitrogen

EZH2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

13 Published Figures

24 References

View all (66) EZH2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

EZH2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

EZH2 Antibody (36-6300) in ICC/IF

Immunofluorescence analysis of EZH2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Anti-EZH2 Polyclonal Antibody (Product # 36-6300) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate... View More

EZH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EZH2 Antibody in Immunohistochemistry (IHC)

EZH2 Antibody in Immunoprecipitation, ChIP Assay (IP, ChIP)

Product Image Gallery

EZH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Knockdown of EZH2 was achieved by transfecting HeLa cells with EZH2 specific siRNAs (Silencer® select Product # S4917 and S4918). ChIP was performed using EZH2 Polyclonal Antibody (Product # 36-6300) on sheared chromatin from HeLa knockdown cells (grey bar), non-specific scrambled siRNA transfected cells (orange bar) and untransfected cells (blue bar). Decrease in fold enrichment of active binding region in siRNA mediated knockdown cells confirms that antibody is specific to EZH2. {KD}

Product Details

36-6300

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 5 publications 5 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533271

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In vivo antagonistic role of the Human T-Cell Leukemia Virus Type 1 regulatory proteins Tax and HBZ.

PLoS pathogens

Akkouche A,Moodad S,Hleihel R,Skayneh H,Chambeyron S,El Hajj H,Bazarbachi A

36-6300 was used in Proximity Ligation Assay (PLA), Immunocytochemistry to establish the in vivo antagonistic effect of HBZ on Tax-induced transformation and cellular effects.

Fri Jan 01 00:00:00 EST 2021

In vivo antagonistic role of the Human T-Cell Leukemia Virus Type 1 regulatory proteins Tax and HBZ.

PLoS pathogens

Akkouche A,Moodad S,Hleihel R,Skayneh H,Chambeyron S,El Hajj H,Bazarbachi A

36-6300 was used in Proximity Ligation Assay (PLA), Immunocytochemistry to establish the in vivo antagonistic effect of HBZ on Tax-induced transformation and cellular effects.

Fri Jan 01 00:00:00 EST 2021

Exosomal lncRNA UCA1 Derived From Pancreatic Stellate Cells Promotes Gemcitabine Resistance in Pancreatic Cancer via the SOCS3/EZH2 Axis.

Frontiers in oncology

Chi Y,Xin H,Liu Z

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in ChIP assay

Thu Apr 28 00:00:00 EDT 2022

Exosomal lncRNA UCA1 Derived From Pancreatic Stellate Cells Promotes Gemcitabine Resistance in Pancreatic Cancer via the SOCS3/EZH2 Axis.

Frontiers in oncology

Chi Y,Xin H,Liu Z

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in ChIP assay

Thu Apr 28 00:00:00 EDT 2022

Exosomal lncRNA UCA1 Derived From Pancreatic Stellate Cells Promotes Gemcitabine Resistance in Pancreatic Cancer via the SOCS3/EZH2 Axis.

Frontiers in oncology

Chi Y,Xin H,Liu Z

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in ChIP assay

Thu Apr 28 00:00:00 EDT 2022

ARID1A alterations and their clinical significance in cholangiocarcinoma.

PeerJ

Namjan A,Techasen A,Loilome W,Sa-Ngaimwibool P,Jusakul A

36-6300 was used in Immunohistochemistry to find that ARID1A inactivation, by somatic mutation or by loss of expression, frequently occurs in liver fluke-related cholangiocarcinoma and is associated with its progression, providing a potential prognostic biomarker and therapeutic target.

Tue Apr 19 00:00:00 EDT 2022

ARID1A alterations and their clinical significance in cholangiocarcinoma.

PeerJ

Namjan A,Techasen A,Loilome W,Sa-Ngaimwibool P,Jusakul A

Tue Apr 19 00:00:00 EDT 2022

PDGFR-β Signaling Regulates Cardiomyocyte Proliferation and Myocardial Regeneration.

Cell reports

Yue Z,Chen J,Lian H,Pei J,Li Y,Chen X,Song S,Xia J,Zhou B,Feng J,Zhang X,Hu S,Nie Y

36-6300 was used in Immunohistochemistry-immunofluorescence to demonstrate that the PDGFR-β/EZH2 pathway is critical for promoting cardiomyocyte proliferation and heart regeneration, providing a potential target for cardiac repair.

Tue Jul 23 00:00:00 EDT 2019

The microRNA signature of patients with sunitinib failure: regulation of UHRF1 pathways by microRNA-101 in renal cell carcinoma.

Oncotarget

Goto Y,Kurozumi A,Nohata N,Kojima S,Matsushita R,Yoshino H,Yamazaki K,Ishida Y,Ichikawa T,Naya Y,Seki N

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Tue Sep 13 00:00:00 EDT 2016

Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung.

Development (Cambridge, England)

Galvis LA,Holik AZ,Short KM,Pasquet J,Lun AT,Blewitt ME,Smyth IM,Ritchie ME,Asselin-Labat ML

36-6300 was used in Immunohistochemistry-immunofluorescence to explore the role of the histone methyltransferase Ezh2 during lung lineage determination.

Wed Apr 15 00:00:00 EDT 2015

Ribavirin as a tri-targeted antitumor repositioned drug.

Oncology reports

De la Cruz-Hernandez E,Medina-Franco JL,Trujillo J,Chavez-Blanco A,Dominguez-Gomez G,Perez-Cardenas E,Gonzalez-Fierro A,Taja-Chayeb L,Dueñas-Gonzalez A

36-6300 was used in western blot to investigate the therapeutic potential of ribavirin as a cancer drug

Fri May 01 00:00:00 EDT 2015

The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis.

The EMBO journal

Wang AH,Zare H,Mousavi K,Wang C,Moravec CE,Sirotkin HI,Ge K,Gutierrez-Cruz G,Sartorelli V

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Wed Apr 17 00:00:00 EDT 2013

Chromatin regulator PRC2 is a key regulator of epigenetic plasticity in glioblastoma.

Cancer research

Natsume A,Ito M,Katsushima K,Ohka F,Hatanaka A,Shinjo K,Sato S,Takahashi S,Ishikawa Y,Takeuchi I,Shimogawa H,Uesugi M,Okano H,Kim SU,Wakabayashi T,Issa JP,Sekido Y,Kondo Y

36-6300 was used in immunohistochemistry to study the role of chromatin regulator PRC2 in epigenetic plasticity in glioblastoma

Mon Jul 15 00:00:00 EDT 2013

Cancer angiogenesis induced by Kaposi sarcoma-associated herpesvirus is mediated by EZH2.

Cancer research

He M,Zhang W,Bakken T,Schutten M,Toth Z,Jung JU,Gill P,Cannon M,Gao SJ

36-6300 was used in immunohistochemistry - paraffin section to demonstrate that elevated EZH2 is associated with epigenetic modifications of Kaposi sarcoma-associated herpesvirus.

Sun Jul 15 00:00:00 EDT 2012

Expression of EZH2 in renal cell carcinoma as a novel prognostic marker.

Pathology international

Lee HW,Choe M

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Thu Nov 01 00:00:00 EDT 2012

Expression of EZH2 in renal cell carcinoma as a novel prognostic marker.

Pathology international

Lee HW,Choe M

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Thu Nov 01 00:00:00 EDT 2012

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

36-6300 was used in ChIP assay to study the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 EDT 2011

UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis.

The EMBO journal

Seenundun S,Rampalli S,Liu QC,Aziz A,Palii C,Hong S,Blais A,Brand M,Ge K,Dilworth FJ

Wed Apr 21 00:00:00 EDT 2010

UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis.

The EMBO journal

Seenundun S,Rampalli S,Liu QC,Aziz A,Palii C,Hong S,Blais A,Brand M,Ge K,Dilworth FJ

Wed Apr 21 00:00:00 EDT 2010

Derepression of CLDN3 and CLDN4 during ovarian tumorigenesis is associated with loss of repressive histone modifications.

Carcinogenesis

Kwon MJ,Kim SS,Choi YL,Jung HS,Balch C,Kim SH,Song YS,Marquez VE,Nephew KP,Shin YK

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Tue Jun 01 00:00:00 EDT 2010

Derepression of CLDN3 and CLDN4 during ovarian tumorigenesis is associated with loss of repressive histone modifications.

Carcinogenesis

Kwon MJ,Kim SS,Choi YL,Jung HS,Balch C,Kim SH,Song YS,Marquez VE,Nephew KP,Shin YK

Published figure using EZH2 polyclonal antibody (Product # 36-6300) in Western Blot

Tue Jun 01 00:00:00 EDT 2010

Chromatin analysis of occluded genes.

Human molecular genetics

Lee JH,Gaetz J,Bugarija B,Fernandes CJ,Snyder GE,Bush EC,Lahn BT

Wed Jul 15 00:00:00 EDT 2009

Expression of stem cell markers in the human fetal kidney.

PloS one

Metsuyanim S,Harari-Steinberg O,Buzhor E,Omer D,Pode-Shakked N,Ben-Hur H,Halperin R,Schneider D,Dekel B

36-6300 was used in immunocytochemistry to identify markers of human fetal kidney stem cells

Fri Aug 21 00:00:00 EDT 2009

MicroRNA-26a targets the histone methyltransferase Enhancer of Zeste homolog 2 during myogenesis.

The Journal of biological chemistry

Wong CF,Tellam RL

Fri Apr 11 00:00:00 EDT 2008

Dynamic changes in histone-methylation 'marks' across the locus encoding interferon-gamma during the differentiation of T helper type 2 cells.

Nature immunology

Chang S,Aune TM

Sun Jul 01 00:00:00 EDT 2007

Dynamic changes in histone-methylation 'marks' across the locus encoding interferon-gamma during the differentiation of T helper type 2 cells.

Nature immunology

Chang S,Aune TM

Sun Jul 01 00:00:00 EDT 2007

Noncoding RNA synthesis and loss of Polycomb group repression accompanies the colinear activation of the human HOXA cluster.

RNA (New York, N.Y.)

Sessa L,Breiling A,Lavorgna G,Silvestri L,Casari G,Orlando V

Thu Feb 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056

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Function(s)

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EZH2 Polyclonal Antibody

EZH2 Antibody (36-6300) in ICC/IF

Product Details

36-6300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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