Active Motif

EZH2 Polyclonal Antibody

EZH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

EZH2 Antibody (39901) in IHC (P)

EZH2 antibody (pAb) tested by Immunohistochemistry Nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone).

EZH2 Antibody in ChIP-Sequencing (ChIP-Seq)

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Product Details

39901

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

5 µL

View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

5 µL

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2614956

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ezh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cells.

Journal of cellular physiology

Moena D,Nardocci G,Acevedo E,Lian J,Stein G,Stein J,Montecino M

Research topic: Transcription

Mon Jun 01 00:00:00 EDT 2020

CBFβ-SMMHC Affects Genome-wide Polycomb Repressive Complex 1 Activity in Acute Myeloid Leukemia.

Cell reports

Cordonnier G,Mandoli A,Cagnard N,Hypolite G,Lhermitte L,Verhoeyen E,Asnafi V,Dillon N,Macintyre E,Martens JHA,Bond J

Research topic: acute myeloid leukemia (AML)

Tue Jan 14 00:00:00 EST 2020

Comprehensive Analysis of Chromatin States in Atypical Teratoid/Rhabdoid Tumor Identifies Diverging Roles for SWI/SNF and Polycomb in Gene Regulation.

Cancer cell

Erkek S,Johann PD,Finetti MA,Drosos Y,Chou HC,Zapatka M,Sturm D,Jones DTW,Korshunov A,Rhyzova M,Wolf S,Mallm JP,Beck K,Witt O,Kulozik AE,Frühwald MC,Northcott PA,Korbel JO,Lichter P,Eils R,Gajjar A,Roberts CWM,Williamson D,Hasselblatt M,Chavez L,Pfister SM,Kool M

Research topic: atypical teratoid rhabdoid tumors (ATRT), Gene Regulation

Mon Jan 14 00:00:00 EST 2019

N-Myc-mediated epigenetic reprogramming drives lineage plasticity in advanced prostate cancer.

The Journal of clinical investigation

Berger A,Brady NJ,Bareja R,Robinson B,Conteduca V,Augello MA,Puca L,Ahmed A,Dardenne E,Lu X,Hwang I,Bagadion AM,Sboner A,Elemento O,Paik J,Yu J,Barbieri CE,Dephoure N,Beltran H,Rickman DS

Research topic: Prostate Cancer

Mon Jul 01 00:00:00 EDT 2019

HiPLA: High-throughput imaging proximity ligation assay.

Methods (San Diego, Calif.)

Serebryannyy LA,Misteli T

Research topic: Methods Paper

Fri Mar 15 00:00:00 EDT 2019

TET1 regulates fibroblast growth factor 8 transcription in gonadotropin releasing hormone neurons.

PloS one

Linscott ML,Chung WCJ

Research topic: Kallmann Syndrome (KS), Gene Regulation

Tue Mar 10 00:00:00 EDT 2020

Disruption of CTCF-YY1-dependent looping of the human papillomavirus genome activates differentiation-induced viral oncogene transcription.

PLoS biology

Pentland I,Campos-León K,Cotic M,Davies KJ,Wood CD,Groves IJ,Burley M,Coleman N,Stockton JD,Noyvert B,Beggs AD,West MJ,Roberts S,Parish JL

Research topic: Virology (HPV)

Mon Oct 01 00:00:00 EDT 2018

EZH2 Inhibition by Tazemetostat Results in Altered Dependency on B-cell Activation Signaling in DLBCL.

Molecular cancer therapeutics

Brach D,Johnston-Blackwell D,Drew A,Lingaraj T,Motwani V,Warholic NM,Feldman I,Plescia C,Smith JJ,Copeland RA,Keilhack H,Chan-Penebre E,Knutson SK,Ribich SA,Raimondi A,Thomenius MJ

Research topic: Lymphoma

Wed Nov 01 00:00:00 EDT 2017

Loss of BAP1 function leads to EZH2-dependent transformation.

Nature medicine

LaFave LM,Béguelin W,Koche R,Teater M,Spitzer B,Chramiec A,Papalexi E,Keller MD,Hricik T,Konstantinoff K,Micol JB,Durham B,Knutson SK,Campbell JE,Blum G,Shi X,Doud EH,Krivtsov AV,Chung YR,Khodos I,de Stanchina E,Ouerfelli O,Adusumilli PS,Thomas PM,Kelleher NL,Luo M,Keilhack H,Abdel-Wahab O,Melnick A,Armstrong SA,Levine RL

Research topic: Cancer

Sun Nov 01 00:00:00 EDT 2015

Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.

Development (Cambridge, England)

Kumar S,Duester G

Research topic: Embryogenesis

Fri Aug 01 00:00:00 EDT 2014

Selective inhibition of EZH2 by EPZ-6438 leads to potent antitumor activity in EZH2-mutant non-Hodgkin lymphoma.

Molecular cancer therapeutics

Knutson SK,Kawano S,Minoshima Y,Warholic NM,Huang KC,Xiao Y,Kadowaki T,Uesugi M,Kuznetsov G,Kumar N,Wigle TJ,Klaus CR,Allain CJ,Raimondi A,Waters NJ,Smith JJ,Porter-Scott M,Chesworth R,Moyer MP,Copeland RA,Richon VM,Uenaka T,Pollock RM,Kuntz KW,Yokoi A,Keilhack H

Research topic: Non-Hodgkin Lympyhoma

Tue Apr 01 00:00:00 EDT 2014

Ezh2 regulates transcriptional and posttranslational expression of T-bet and promotes Th1 cell responses mediating aplastic anemia in mice.

Journal of immunology (Baltimore, Md. : 1950)

Tong Q,He S,Xie F,Mochizuki K,Liu Y,Mochizuki I,Meng L,Sun H,Zhang Y,Guo Y,Hexner E,Zhang Y

Research topic: aplastic anemia, Autoimmunity

Sun Jun 01 00:00:00 EDT 2014

Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas.

Cancer cell

Bender S,Tang Y,Lindroth AM,Hovestadt V,Jones DT,Kool M,Zapatka M,Northcott PA,Sturm D,Wang W,Radlwimmer B,Højfeldt JW,Truffaux N,Castel D,Schubert S,Ryzhova M,Seker-Cin H,Gronych J,Johann PD,Stark S,Meyer J,Milde T,Schuhmann M,Ebinger M,Monoranu CM,Ponnuswami A,Chen S,Jones C,Witt O,Collins VP,von Deimling A,Jabado N,Puget S,Grill J,Helin K,Korshunov A,Lichter P,Monje M,Plass C,Cho YJ,Pfister SM

Research topic: Glioma

Mon Nov 11 00:00:00 EST 2013

ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression.

Cancer cell

Abdel-Wahab O,Adli M,LaFave LM,Gao J,Hricik T,Shih AH,Pandey S,Patel JP,Chung YR,Koche R,Perna F,Zhao X,Taylor JE,Park CY,Carroll M,Melnick A,Nimer SD,Jaffe JD,Aifantis I,Bernstein BE,Levine RL

Research topic: Myeloid Leukemia

Tue Aug 14 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056

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Function(s)

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EZH2 Polyclonal Antibody

EZH2 Antibody (39901) in IHC (P)

Product Details

39901

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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