Invitrogen

EZH2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

13 References

View all (66) EZH2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

EZH2 Antibody in ChIP-Sequencing (ChIP-seq)

FIGURE: 1 / 17

EZH2 Antibody (49-1043) in ChIP-seq

ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the anti-EZH2 antibody (Product # 49-1043). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and ... View More

EZH2 Antibody in Immunocytochemistry (ICC/IF)

EZH2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

EZH2 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Product Image Gallery

EZH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with EZH2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-EZH2 Polyclonal Antibody (Product # 49-1043). {KD}

Product Details

49-1043

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

View 1 publication 1 publication

ChIP assay (ChIP)

1-10 µg/4x10^6 cells

View 4 publications 4 publications

ChIP-sequencing (ChIP-Seq)

2 µg/4x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

N-terminus (aa1-343) of the mouse Ezh2 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% ProClin 300, 0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_2533892

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCs.

Apoptosis : an international journal on programmed cell death

Zhang F,Luo H,Peng W,Wang L,Wang T,Xie Z,Zhang J,Dong W,Zheng X,Liu G,Zhu X,Kang Q,Tian X

49-1043 was used in Western Blotting to improve the regulatory mechanism of LncRNA Tmem235 during hypoxic apoptosis of BMSCs and provide a more complete theoretical pathway for targeting LncRNA to inhibit hypoxic apoptosis of BMSCs.

Sat Oct 01 00:00:00 EDT 2022

Demethylation of H3K9 and H3K27 Contributes to the Tubular Renal Damage Triggered by Endoplasmic Reticulum Stress.

Antioxidants (Basel, Switzerland)

Diaz-Bulnes P,Saiz ML,Corte-Iglesias V,Rodrigues-Diez RR,Bernardo Florez A,Ruiz Bernet C,Martin Martin C,Ruiz-Ortega M,Suarez-Alvarez B,López-Larrea C

49-1043 was used in Chromatin immunoprecipitation to investigate whether epigenetic dynamics mediated by histone H3K9 and H3K27 methylation might help to reduce or inhibit the exacerbated and maladaptive UPR triggered in tubular epithelial cells.

Tue Jul 12 00:00:00 EDT 2022

HOPX regulates bone marrow-derived mesenchymal stromal cell fate determination via suppression of adipogenic gene pathways.

Scientific reports

Hng CH,Camp E,Anderson P,Breen J,Zannettino A,Gronthos S

49-1043 was used in Chromatin immunoprecipitation to study the role of homeodomain-only protein homeobox (HOPX) gene expression during bone marrow stromal cell (BMSC) growth and differentiation.

Thu Jul 09 00:00:00 EDT 2020

HOPX regulates bone marrow-derived mesenchymal stromal cell fate determination via suppression of adipogenic gene pathways.

Scientific reports

Hng CH,Camp E,Anderson P,Breen J,Zannettino A,Gronthos S

49-1043 was used in Chromatin immunoprecipitation to study the role of homeodomain-only protein homeobox (HOPX) gene expression during bone marrow stromal cell (BMSC) growth and differentiation.

Thu Jul 09 00:00:00 EDT 2020

Methylation and PTEN activation in dental pulp mesenchymal stem cells promotes osteogenesis and reduces oncogenesis.

Nature communications

Shen WC,Lai YC,Li LH,Liao K,Lai HC,Kao SY,Wang J,Chuong CM,Hung SC

49-1043 was used in Western Blotting to demonstrate how several epigenetic factors broadly affect lineage commitment and tumorigenesis, which should be considered when developing therapeutic uses of stem cells.

Mon May 20 00:00:00 EDT 2019

CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor.

Oncogene

Wu L,Runkle C,Jin HJ,Yu J,Li J,Yang X,Kuzel T,Lee C,Yu J

49-1043 was used in ChIP assay to report a tumor-suppressive role of NOV in prostate cancer.

Thu Jan 23 00:00:00 EST 2014

The central role of EED in the orchestration of polycomb group complexes.

Nature communications

Cao Q,Wang X,Zhao M,Yang R,Malik R,Qiao Y,Poliakov A,Yocum AK,Li Y,Chen W,Cao X,Jiang X,Dahiya A,Harris C,Feng FY,Kalantry S,Qin ZS,Dhanasekaran SM,Chinnaiyan AM

49-1043 was used in ChIP assay and western blot to study the Involvement of EED in the organization of polycomb group complexes

Tue Oct 27 00:00:00 EDT 2015

The central role of EED in the orchestration of polycomb group complexes.

Nature communications

Cao Q,Wang X,Zhao M,Yang R,Malik R,Qiao Y,Poliakov A,Yocum AK,Li Y,Chen W,Cao X,Jiang X,Dahiya A,Harris C,Feng FY,Kalantry S,Qin ZS,Dhanasekaran SM,Chinnaiyan AM

49-1043 was used in ChIP assay and western blot to study the Involvement of EED in the organization of polycomb group complexes

Tue Oct 27 00:00:00 EDT 2015

Histone-modifying genes as biomarkers in hepatocellular carcinoma.

International journal of clinical and experimental pathology

Hung SY,Lin HH,Yeh KT,Chang JG

49-1043 was used in immunohistochemistry - paraffin section to correlate histone-modifying genes and prognosis of hepatocellular carcinoma to identify out new diagnostic markers

Mon Feb 23 00:00:00 EST 2015

Histone-modifying genes as biomarkers in hepatocellular carcinoma.

International journal of clinical and experimental pathology

Hung SY,Lin HH,Yeh KT,Chang JG

49-1043 was used in immunohistochemistry - paraffin section to correlate histone-modifying genes and prognosis of hepatocellular carcinoma to identify out new diagnostic markers

Mon Feb 23 00:00:00 EST 2015

EZH2 regulates expression of p57 and contributes to progression of ovarian cancer in vitro and in vivo.

Cancer science

Guo J,Cai J,Yu L,Tang H,Chen C,Wang Z

Tue Mar 01 00:00:00 EST 2011

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Thu Oct 21 00:00:00 EDT 2010

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Thu Oct 21 00:00:00 EDT 2010

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Thu Oct 21 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056

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Function(s)

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EZH2 Polyclonal Antibody

EZH2 Antibody (49-1043) in ChIP-seq

Product Details

49-1043

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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