Active Motif

EZH2 Monoclonal Antibody (AC22)

FIGURE: 1 / 1

EZH2 Antibody (39875) in ChIP

EZH2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of EZH2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using a primer pair specific for the Myt1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

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Product Details

39875

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µg

View 5 publications 5 publications

ChIP-sequencing (ChIP-Seq)

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

AC22

Immunogen

This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

70mM tris, pH 8, with 30% glycerol, 0.07mM EDTA, 31mM glycine, 105mM NaCl

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2561022

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The TGFB2-AS1 lncRNA Regulates TGF-β Signaling by Modulating Corepressor Activity.

Cell reports

Papoutsoglou P,Tsubakihara Y,Caja L,Morén A,Pallis P,Ameur A,Heldin CH,Moustakas A

Research topic: Gene Regulation, Transcriptomics

Tue Sep 17 00:00:00 EDT 2019

Identification and removal of sequencing artifacts produced by mispriming during reverse transcription in multiple RNA-seq technologies.

RNA (New York, N.Y.)

Shivram H,Iyer VR

Research topic: Transcription Regulation

Sat Sep 01 00:00:00 EDT 2018

Chd8 mediates cortical neurogenesis via transcriptional regulation of cell cycle and Wnt signaling.

Nature neuroscience

Durak O,Gao F,Kaeser-Woo YJ,Rueda R,Martorell AJ,Nott A,Liu CY,Watson LA,Tsai LH

Research topic: Brain & Nerves - Autism

Tue Nov 01 00:00:00 EDT 2016

LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection.

PLoS pathogens

Toth Z,Papp B,Brulois K,Choi YJ,Gao SJ,Jung JU

Research topic: HIV & AIDS

Thu Sep 01 00:00:00 EDT 2016

LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection.

PLoS pathogens

Toth Z,Papp B,Brulois K,Choi YJ,Gao SJ,Jung JU

Research topic: HIV & AIDS

Thu Sep 01 00:00:00 EDT 2016

LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection.

PLoS pathogens

Toth Z,Papp B,Brulois K,Choi YJ,Gao SJ,Jung JU

Research topic: HIV & AIDS

Thu Sep 01 00:00:00 EDT 2016

Non-Canonical EZH2 Transcriptionally Activates RelB in Triple Negative Breast Cancer.

PloS one

Lawrence CL,Baldwin AS

Research topic: Breast Cancer

Tue Jun 06 00:00:00 EDT 2017

The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.

Nature immunology

Ranzani V,Rossetti G,Panzeri I,Arrigoni A,Bonnal RJ,Curti S,Gruarin P,Provasi E,Sugliano E,Marconi M,De Francesco R,Geginat J,Bodega B,Abrignani S,Pagani M

Research topic: Immunology

Sun Mar 01 00:00:00 EST 2015

The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.

Nature immunology

Ranzani V,Rossetti G,Panzeri I,Arrigoni A,Bonnal RJ,Curti S,Gruarin P,Provasi E,Sugliano E,Marconi M,De Francesco R,Geginat J,Bodega B,Abrignani S,Pagani M

Research topic: Immunology

Sun Mar 01 00:00:00 EST 2015

Tumor-secreted Hsp90 subverts polycomb function to drive prostate tumor growth and invasion.

The Journal of biological chemistry

Nolan KD,Franco OE,Hance MW,Hayward SW,Isaacs JS

Research topic: Prostate Cancer

Fri Mar 27 00:00:00 EDT 2015

Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells.

PLoS pathogens

Rossetto CC,Tarrant-Elorza M,Pari GS

Research topic: Innate Immunity

Mon Dec 09 00:00:00 EST 2013

Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program.

The Journal of biological chemistry

Lin S,Shen H,Li JL,Tang S,Gu Y,Chen Z,Hu C,Rice JC,Lu J,Wu L

Research topic: Transcription Regulation

Fri Mar 01 00:00:00 EST 2013

Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome.

PloS one

Bengani H,Mendiratta S,Maini J,Vasanthi D,Sultana H,Ghasemi M,Ahluwalia J,Ramachandran S,Mishra RK,Brahmachari V

Research topic: Transcription Regulation

Fri Oct 06 00:00:00 EDT 2017

BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression.

PLoS pathogens

Woellmer A,Arteaga-Salas JM,Hammerschmidt W

Research topic: Transcription Regulation

Sat Sep 01 00:00:00 EDT 2012

Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.

Journal of virology

Murata T,Kondo Y,Sugimoto A,Kawashima D,Saito S,Isomura H,Kanda T,Tsurumi T

Research topic: Virology (EBV)

Tue May 01 00:00:00 EDT 2012

Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.

Journal of virology

Murata T,Kondo Y,Sugimoto A,Kawashima D,Saito S,Isomura H,Kanda T,Tsurumi T

Research topic: Virology (EBV)

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056

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Function(s)

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EZH2 Monoclonal Antibody (AC22)

EZH2 Antibody (39875) in ChIP

Product Details

39875

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

RNA Immunoprecipitation (RIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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