Invitrogen

EZH2 Monoclonal Antibody (AC22), eFluor™ 660, eBioscience™

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Datasheet
Protocols
Questions & Answers

Product Details

50-9867-82

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

20 µg/mL
-

Flow Cytometry (Flow)

-
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AC22

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574400

Product Specific Information

Description: The monoclonal antibody AC22 recognizes EZH2 (Enhancer of zeste homolog 2), a member of the polycomb group (PcG) proteins responsible for repression of gene expression through recognition and modification of histone methylation and chromatin structure. EZH2 comprises the catalytic subunit of the polycomb repressive complex 2 (PRC2) and directly represses gene expression through methylation of histone H3 on lysine 27 (H3K27) as well as direct protein targets such as GATA4. The PRC2 complex gene repression is necessary for proper development and differentiation. Underexpression of EZH2 is linked to abnormal developmental patterning and a loss of stem cell pluripotency, while overexpression of EZH2 is present in many tumors and correlates with poor prognosis in melanoma, breast, and prostate cancer.

Applications Reported: This AC22 antibody has been reported for use in microscopy, and immunocytochemistry.

Applications Tested: This AC22 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Rat) 312299, (Mouse) 14056

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Function(s)
core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme
Process(es)
negative regulation of transcription from RNA polymerase II promoter DNA methylation chromatin organization transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of epithelial to mesenchymal transition regulation of gliogenesis skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration cerebellar cortex development hippocampus development response to estradiol negative regulation of transcription elongation from RNA polymerase II promoter regulation of cell proliferation regulation of circadian rhythm positive regulation of MAP kinase activity negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of GTPase activity negative regulation of epidermal cell differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated negative regulation of retinoic acid receptor signaling pathway rhythmic process negative regulation of striated muscle cell differentiation cellular response to hydrogen peroxide G1 to G0 transition histone H3-K27 methylation protein localization to chromatin positive regulation of protein serine/threonine kinase activity positive regulation of dendrite development negative regulation of G1/S transition of mitotic cell cycle regulation of protein phosphorylation regulation of transcription from RNA polymerase II promoter regulation of gene expression negative regulation of gene expression histone methylation regulation of neurogenesis chromatin modification peptidyl-lysine methylation methylation
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