Invitrogen

EZH2 Monoclonal Antibody (H.547.3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

10 Published Figures

7 References

View all (66) EZH2 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FIGURE: 1 / 14

EZH2 Antibody (MA5-15101) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), HCT 116 (Lane 3), SW480 (Lane 4), Caco-2 (Lane 5), T98G (Lane 6), PC-3 (Lane 7), DU 145 (Lane 8), Jurkat (Lane9) and Hep G2 (Lane 10). The blot was probed with Anti-EZH2 Monoclonal Antibody (H.547.3) (Product # MA5-15101, 1:1000 dilution) and detected by chemiluminescence using Goat anti Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). An 86 kDa band corresponding to... View More

EZH2 Antibody in Immunocytochemistry (ICC/IF)

EZH2 Antibody in Immunohistochemistry (IHC)

EZH2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

EZH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with EZH2 siRNA and reduction of signal was observed in Western Blot using EZH2 Monoclonal Antibody (H.547.3) (Product # MA5-15101). {KD}

Product Details

MA5-15101

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

H.547.3

Immunogen

Ezh2 recombinant protein containing human Ezh2 residues 351-453

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

403 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11004378

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuroprotective effects of GSK-343 in an in vivo model of MPTP-induced nigrostriatal degeneration.

Journal of neuroinflammation

Mannino D,Scuderi SA,Casili G,Bova V,Cucinotta L,Lanza M,Filippone A,Esposito E,Paterniti I

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Fri Jun 30 00:00:00 EDT 2023

Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2.

Journal of the American Heart Association

Cheng Z,Naga Srikanth Garikipati V,Truongcao MM,Cimini M,Huang G,Wang C,Benedict C,Gonzalez C,Mallaredy V,Goukassian DA,Verma SK,Kishore R

MA5-15101 was used in Immunohistochemistry to study the role of serum-derived small extracellular vesicles (ssEVs) in diabetes mellitus-impaired microvascular endothelial cell function.

Tue May 18 00:00:00 EDT 2021

Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2.

Journal of the American Heart Association

Cheng Z,Naga Srikanth Garikipati V,Truongcao MM,Cimini M,Huang G,Wang C,Benedict C,Gonzalez C,Mallaredy V,Goukassian DA,Verma SK,Kishore R

MA5-15101 was used in Immunohistochemistry to study the role of serum-derived small extracellular vesicles (ssEVs) in diabetes mellitus-impaired microvascular endothelial cell function.

Tue May 18 00:00:00 EDT 2021

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

MA5-15101 was used in Immunocytochemistry to study the role of EZH2-mediated trimethylation of histone H3 lysine 27(EZH2-H3K27me3) in the proliferation of psoriatic keratinocyte.

Sat Oct 03 00:00:00 EDT 2020

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

MA5-15101 was used in Immunocytochemistry to study the role of EZH2-mediated trimethylation of histone H3 lysine 27(EZH2-H3K27me3) in the proliferation of psoriatic keratinocyte.

Sat Oct 03 00:00:00 EDT 2020

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

MA5-15101 was used in Immunocytochemistry to study the role of EZH2-mediated trimethylation of histone H3 lysine 27(EZH2-H3K27me3) in the proliferation of psoriatic keratinocyte.

Sat Oct 03 00:00:00 EDT 2020

Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2.

The Journal of clinical investigation

Wang J,Cheng P,Pavlyukov MS,Yu H,Zhang Z,Kim SH,Minata M,Mohyeldin A,Xie W,Chen D,Goidts V,Frett B,Hu W,Li H,Shin YJ,Lee Y,Nam DH,Kornblum HI,Wang M,Nakano I

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Tue Aug 01 00:00:00 EDT 2017

Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes.

PLoS genetics

Wongtrakoongate P,Riddick G,Fucharoen S,Felsenfeld G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Immunoprecipitation

Thu Oct 01 00:00:00 EDT 2015

Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes.

PLoS genetics

Wongtrakoongate P,Riddick G,Fucharoen S,Felsenfeld G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Immunoprecipitation

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Rat) 312299, (Mouse) 14056

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Function(s)

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EZH2 Monoclonal Antibody (H.547.3)

EZH2 Antibody (MA5-15101) in WB

Product Details

MA5-15101

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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