Invitrogen

Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), Biotin, eBioscience™

Ea52-68 peptide bound to I-Ab Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

Ea52-68 peptide bound to I-Ab Antibody (13-5741-82) in Flow

Staining of peripheral blood from a C57BL/6 mouse (left) or a mouse strain known to express the E alpha 52-68 peptide:I-A (b) complex (right) with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Anti-Mouse E alpha 52-68 peptide bound to I-Ab Biotin followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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Product Details

13-5741-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioY-Ae (YAe, Y-Ae)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657821

Product Specific Information

Description: The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The Y-Ae antibody detects a determinant expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determinant is expressed on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the Y-Ae determinant is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. The antibody does not react with invariant chain-associated class II MHC complexes.

The reactivity of the Y-Ae antibody is MHC-restricted. The Y-Ae antibody was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts. In this strain combination, there is incompatibility for I-Ab and I-Eb molecules. The antibody reacts with 10-15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., strains B10.A(5R) and B10.A(3R)). The antibody does not react with I-A molecules in strains that have a non-functional I-E (a chain) gene (e.g., strains B6 and B10), nor with mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1), nor with any other strain tested. The Y-Ae antibody recognizes an Ea peptide (Ea 52-68) bound to I-Ab molecules, but not to Ea 52-68 peptide bound to I-Ak or I-Abm-12 molecules.

Applications Reported: This eBioY-Ae (YAe, Y-Ae) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioY-Ae (YAe, Y-Ae) antibody has been tested by flow cytometric analysis of a mouse strain known to express the I-A^b/MHC Class II:Ea peptide complex. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Club Cell TRPV4 Serves as a Damage Sensor Driving Lung Allergic Inflammation.

Cell host & microbe

Wiesner DL,Merkhofer RM,Ober C,Kujoth GC,Niu M,Keller NP,Gern JE,Brockman-Schneider RA,Evans MD,Jackson DJ,Warner T,Jarjour NN,Esnault SJ,Feldman MB,Freeman M,Mou H,Vyas JM,Klein BS

13-5741 was used in Flow cytometry/Cell sorting to conclude that club cells detect junction damage as mechanical stress, which signals danger via TRPV4, calcium, and calcineurin to initiate allergic sensitization.

Wed Apr 08 00:00:00 EDT 2020

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using Ea52-68 peptide bound to I-Ab monoclonal antibody (Product # 13-5741-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes.

The EMBO journal

Fletcher K,Ulferts R,Jacquin E,Veith T,Gammoh N,Arasteh JM,Mayer U,Carding SR,Wileman T,Beale R,Florey O

13-5741 was used in Flow cytometry/Cell sorting to demonstrate activation of non-canonical autophagy dependent on the WD40 CTD during influenza A virus infection.

Thu Feb 15 00:00:00 EST 2018

Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation.

Nature communications

Bonacina F,Coe D,Wang G,Longhi MP,Baragetti A,Moregola A,Garlaschelli K,Uboldi P,Pellegatta F,Grigore L,Da Dalt L,Annoni A,Gregori S,Xiao Q,Caruso D,Mitro N,Catapano AL,Marelli-Berg FM,Norata GD

Mon Aug 06 00:00:00 EDT 2018

Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo.

eLife

Benson RA,MacLeod MK,Hale BG,Patakas A,Garside P,Brewer JM

13-5741 was used in Flow cytometry/Cell sorting to investigate the conditions affecting follicular helper T cell differentiation and antigen presentation in an influenza infection model.

Mon Aug 10 00:00:00 EDT 2015

Soluble antigen traffics rapidly and selectively from the corneal surface to the eye draining lymph node and activates T cells when codelivered with CpG oligonucleotides.

Journal of leukocyte biology

Dang Z,Kuffová L,Liu L,Forrester JV

Sat Mar 01 00:00:00 EST 2014

Innate lymphoid cells regulate CD4+ T-cell responses to intestinal commensal bacteria.

Nature

Hepworth MR,Monticelli LA,Fung TC,Ziegler CG,Grunberg S,Sinha R,Mantegazza AR,Ma HL,Crawford A,Angelosanto JM,Wherry EJ,Koni PA,Bushman FD,Elson CO,Eberl G,Artis D,Sonnenberg GF

13-5741 was used in Flow cytometry/Cell sorting to identify that innate lymphoid cells maintain intestinal homeostasis through MHCII-dependent interactions with CD4(+) T cells.

Thu Jun 06 00:00:00 EDT 2013

Adaptor protein-3 in dendritic cells facilitates phagosomal toll-like receptor signaling and antigen presentation to CD4(+) T cells.

Immunity

Mantegazza AR,Guttentag SH,El-Benna J,Sasai M,Iwasaki A,Shen H,Laufer TM,Marks MS

13-5741 was used in Flow cytometry/Cell sorting to propose that AP-3-dependent TLR delivery from endosomes to phagosomes and subsequent signaling mobilize peptide:MHC-II export from intracellular stores.

Fri May 25 00:00:00 EDT 2012

Immune insufficiency during GVHD is due to defective antigen presentation within dendritic cell subsets.

Blood

Markey KA,Koyama M,Kuns RD,Lineburg KE,Wilson YA,Olver SD,Raffelt NC,Don AL,Varelias A,Robb RJ,Cheong M,Engwerda CR,Steptoe RJ,Ramshaw HS,Lopez AF,Vega-Ramos J,Lew AM,Villadangos JA,Hill GR,MacDonald KP

13-5741 was used in Flow cytometry/Cell sorting to investigate the cause of immune suppression during graft-versus-host disease.

Thu Jun 14 00:00:00 EDT 2012

Alum increases antigen uptake, reduces antigen degradation and sustains antigen presentation by DCs in vitro.

Immunology letters

Ghimire TR,Benson RA,Garside P,Brewer JM

13-5741 was used in Flow cytometry/Cell sorting to demonstrate that alum induces higher antigen accumulation and increased antigen presentation by dendritic cells in vitro.

Sat Sep 01 00:00:00 EDT 2012

In vivo requirement for Atg5 in antigen presentation by dendritic cells.

Immunity

Lee HK,Mattei LM,Steinberg BE,Alberts P,Lee YH,Chervonsky A,Mizushima N,Grinstein S,Iwasaki A

13-5741 was used in Flow cytometry/Cell sorting to investigate how dendritic cells utilise autophagic machinery to optimally present extracellular microbial antigens for MHC II molecules.

Fri Feb 26 00:00:00 EST 2010

Vaccinia virus A35R inhibits MHC class II antigen presentation.

Virology

Rehm KE,Connor RF,Jones GJ,Yimbu K,Roper RL

13-5741 was used in Flow cytometry/Cell sorting to identify a function for the A35 protein in virulence and identify a VV gene that inhibits peptide antigen presentation.

Fri Feb 05 00:00:00 EST 2010

Actin-binding protein 1 regulates B cell receptor-mediated antigen processing and presentation in response to B cell receptor activation.

Journal of immunology (Baltimore, Md. : 1950)

Onabajo OO,Seeley MK,Kale A,Qualmann B,Kessels M,Han J,Tan TH,Song W

13-5741 was used in Flow cytometry/Cell sorting to demonstrate that BCR regulates Abp1 by inducing phosphorylation and actin cytoskeleton rearrangement, and that Abp1 facilitates BCR-mediated Ag processing.

Thu May 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; H-2Eb; H2Eb; histocompatibility 2, class II antigen A, beta 1; Ia-4; Ia4; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

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Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

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UniProt ID: (Mouse) P14483

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Entrez Gene ID: (Mouse) 14961

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Function(s)

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding major histocompatibility complex protein

Process(es)

B cell affinity maturation immune system process immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin antigen processing and presentation of peptide or polysaccharide antigen via MHC class II positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of T cell proliferation positive regulation of alpha-beta T cell activation antigen processing and presentation of peptide antigen cellular response to interferon-gamma

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), Biotin, eBioscience™

Ea52-68 peptide bound to I-Ab Antibody (13-5741-82) in Flow

Product Details

13-5741-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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