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Eaat2 Antibody (600-101-MN3) in ICC/IF
Immunofluorescence of Goat Anti-Eaat2 Antibody. Cells: Rat Pups Brain heterogenous cells. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary Antibody: Anti-Eaat2 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) anti-Eaat2 and secondary, (C) merge A+B, (D) secondary only. Expected localization: plasma membrane >> extracellular > mitochondria, nucleus.
Product Details
600-101-MN3
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Eaat2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of Rat Eaat2 surrounding aa1-25 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against rat Eaat2. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.
Target Information
Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2; solute carrier family 1, member 2
Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; Eaat2; Glt; GLT-1; GLT1; MGLT1; Slc1a2
UniProt ID: (Mouse) P43006, (Rat) P31596
Entrez Gene ID: (Mouse) 20511, (Rat) 29482
transport
dicarboxylic acid transport
nervous system development
visual behavior
response to light stimulus
response to wounding
multicellular organism aging
L-glutamate transport
telencephalon development
adult behavior
cellular response to extracellular stimulus
multicellular organism growth
sodium ion transmembrane transport
response to drug
response to amino acid
positive regulation of glucose import
L-glutamate import
D-aspartate import
L-glutamate transmembrane transport
neurotransmitter transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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