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Elongin B Antibody (BS-5136R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongin B Polyclonal Antibody, Unconjugated (bs-5136R) at 1:200 overnight at 4°C, DAB staining.
Product Details
BS-5136R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Elongin B, amino acids 10-100.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa, elongin B; EloB; Elongin 18 kDa subunit; elongin B; elongin, 18-kD subunit; Elongin-B; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII p18; TCEB 2; transcription elongation factor B (SIII) polypeptide 2 (18kD, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18 kDa, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Transcription elongation factor B polypeptide 2
Gene Aliases: 0610040H15Rik; ELOB; SIII; TCEB2
UniProt ID: (Human) Q15370, (Mouse) P62869, (Rat) P62870
Entrez Gene ID: (Human) 6923, (Mouse) 67673, (Rat) 81807
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein complex assembly
protein ubiquitination
regulation of transcription from RNA polymerase II promoter in response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
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