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Endostatin Antibody (500-P262BT-25UG) in ELISA
Sandwich ELISA: To detect hEndostatin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Endostatin Polyclonal Antibody, Biotin (Product # 500-P262BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Endostatin Polyclonal Antibody (Product # 500-P262-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEndostatin.
Product Details
500-P262BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Endostatin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930143
Product Specific Information
AA Sequence of recombinant protein: MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Endostatin. Anti-Human Endostatin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hEndostatin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Endostatin (500-P262) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEndostatin.
Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEndostatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P262BT-1MG will be provided as 2 x 500 µg
Target Information
Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antiangiogenic agent; Collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; endostatin; FLJ27325; FLJ34914; MGC74745; multi-functional protein MFP
Gene Aliases: COL18A1; KNO; KNO1; KS
UniProt ID: (Human) Q9Y6Q7
Entrez Gene ID: (Human) 80781
angiogenesis
endothelial cell morphogenesis
cell adhesion
visual perception
positive regulation of cell proliferation
negative regulation of cell proliferation
organ morphogenesis
extracellular matrix organization
positive regulation of cell migration
collagen catabolic process
response to drug
response to hydrostatic pressure
positive regulation of endothelial cell apoptotic process
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