Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
Eotaxin 2 Antibody (500-P103GBT-25UG) in ELISA
Sandwich ELISA: To detect Human Eotaxin-2 (CCL24) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Eotaxin 2 Polyclonal Antibody, Biotin (Product # 500-P103GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Eotaxin 2 Polyclonal Antibody (Product # 500-P103G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-2 (CCL24).
Product Details
500-P103GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Eotaxin-2 (CCL24)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929201
Product Specific Information
AA Sequence of recombinant protein: VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKGGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-2 (CCL24). Anti-Human Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Eotaxin-2 (CCL24) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin-2 (CCL24) (500-P103G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-2 (CCL24).
Western Blot: To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P103GBT-1MG will be provided as 2 x 500 µg
Target Information
Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 24; chemokine (C-C motif) ligand 24; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin-2; Eotaxin2; MPIF-2; Myeloid progenitor inhibitory factor 2; small inducible cytokine subfamily A (Cys-Cys), member 24; Small-inducible cytokine A24
Gene Aliases: CCL24; Ckb-6; MPIF-2; MPIF2; SCYA24
UniProt ID: (Human) O00175
Entrez Gene ID: (Human) 6369
positive regulation of endothelial cell proliferation
monocyte chemotaxis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
positive regulation of cell migration
neutrophil chemotaxis
positive regulation of actin filament polymerization
positive regulation of GTPase activity
positive regulation of angiogenesis
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of eosinophil migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support