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Eotaxin 2 Antibody (500-P175GBT-25UG) in ELISA
Sandwich ELISA: To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Eotaxin 2 Polyclonal Antibody, Biotin (Product # 500-P175GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Eotaxin 2 Polyclonal Antibody (Product # 500-P175G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin-2.
Product Details
500-P175GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine Eotaxin-2 (CCL24)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929646
Product Specific Information
AA Sequence of recombinant protein: VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine Eotaxin-2 (CCL24). Anti-Murine Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin-2 (500-P175G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin-2.
Western Blot: To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P175GBT-1MG will be provided as 2 x 500 µg
Target Information
Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 24; CC chemokine CCL24; Eosinophil chemotactic protein 2; Eotaxin-2; Eotaxin2; small inducible cytokine A24; Small-inducible cytokine A24
Gene Aliases: Ccl24; CKb-6; MPIF-2; Scya24
UniProt ID: (Mouse) Q9JKC0
Entrez Gene ID: (Mouse) 56221
positive regulation of endothelial cell proliferation
chemotaxis
inflammatory response
immune response
cytoskeleton organization
regulation of cell shape
positive regulation of cell migration
positive regulation of actin filament polymerization
positive regulation of GTPase activity
positive regulation of angiogenesis
eosinophil chemotaxis
positive regulation of inflammatory response
positive regulation of eosinophil migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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