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Eotaxin 3 Antibody (500-P156GBT-25UG) in WB
Western Blot: To detect hEotaxin-3 by Western Blot analysis Eotaxin 3 Polyclonal Antibody, Biotin (Product # 500-P156GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P156GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Eotaxin-3 (CCL26)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929532
Product Specific Information
AA Sequence of recombinant protein: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-3 (CCL26). Anti-hEotaxin-3 (CCL26)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hEotaxin-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin-3 (500-P156G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin-3.
Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P156GBT-1MG will be provided as 2 x 500 µg
Target Information
Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 26; CC chemokine IMAC; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin-3; Eotaxin3; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4-alpha; PRO242; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; Thymic stroma chemokine-1; TSC-1; UNQ216
Gene Aliases: CCL26; IMAC; MIP-4a; MIP-4alpha; SCYA26; TSC-1; UNQ216/PRO242
UniProt ID: (Human) Q9Y258
Entrez Gene ID: (Human) 10344
positive regulation of endothelial cell proliferation
monocyte chemotaxis
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell migration
neutrophil chemotaxis
positive regulation of actin filament polymerization
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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