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Eotaxin Antibody (500-P67-1MG) in ELISA
Sandwich ELISA: To detect Murine Eotaxin (CCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Eotaxin Polyclonal Antibody (Product # 500-P67-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Eotaxin Polyclonal Antibody, Biotin (Product # 500-P67BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Eotaxin (CCL11).
Product Details
500-P67-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine Eotaxin (CCL11)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930696
Product Specific Information
AA Sequence of recombinant protein: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Eotaxin (CCL11). Anti-Murine Eotaxin (CCL11)-specific antibody was purified by affinity chromatography employing an immobilized Murine Eotaxin (CCL11) matrix.
Sandwich ELISA: To detect Murine Eotaxin (CCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine Eotaxin (500-P67Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Eotaxin (CCL11).
Western Blot: To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 11; Eosinophil chemotactic protein; Eotaxin; Eotaxin 1; Eotaxin1; MGC22554; small chemokine (C-C motif) ligand 11; small inducible cytokine A11; Small-inducible cytokine A11
Gene Aliases: Ccl11; eotaxin; Scya11
UniProt ID: (Mouse) P48298
Entrez Gene ID: (Mouse) 20292
positive regulation of endothelial cell proliferation
mast cell chemotaxis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
actin filament organization
regulation of cell shape
positive regulation of cell migration
positive regulation of actin filament polymerization
positive regulation of GTPase activity
positive regulation of angiogenesis
eosinophil chemotaxis
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
ERK1 and ERK2 cascade
Performance Guarantee
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