Invitrogen

EpCAM Monoclonal Antibody (323/A3)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

18 References

View all (275) EpCAM (CD326) antibodies

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Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

EpCAM Antibody in Immunocytochemistry (ICC/IF)

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EpCAM Antibody (MA5-12436) in ICC/IF

Immunofluorescence analysis of EpCAM was performed using HT-29 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA5-12436) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A28175) at a dilution of 1:... View More

EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EpCAM Antibody in Immunohistochemistry (IHC)

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EpCAM Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EpCAM was observed in HT-29 cells in plasma membrane, which is a positive model for EpCAM expression, in comparison to BJ (null for EpCAM expression) using Anti-EpCAM Mouse Monoclonal Antibody (Product # MA5-12436) in immunofluorescence. {RE}

Product Details

MA5-12436

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:10-1:200

View 6 publications 6 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 2 publications 2 publications

Radioimmune Assays (RIA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

323/A3

Immunogen

MCF-7 human breast cancer cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981962

Product Specific Information

MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.

The MA5-12436 immunogen is mCF-7 human breast cancer cells.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of Novel Candidate Genes for Familial Thyroid Cancer by Whole Exome Sequencing.

International journal of molecular sciences

Tous C,Muñoz-Redondo C,Bravo-Gil N,Gavilan A,Fernández RM,Antiñolo J,Navarro-González E,Antiñolo G,Borrego S

Published figure using EpCAM monoclonal antibody (Product # MA5-12436) in Immunohistochemistry (Paraffin)

Tue Apr 25 00:00:00 EDT 2023

Targeted activation in localized protein environments via deep red photoredox catalysis.

Nature chemistry

Tay NES,Ryu KA,Weber JL,Olow AK,Cabanero DC,Reichman DR,Oslund RC,Fadeyi OO,Rovis T

MA5-12436 was used in Western Blot to identify a small subset of proteins with previously known and unknown association to EpCAM, including CDH3, a clinically relevant protein that shares high tumor-selective expression with EpCAM.

Sun Jan 01 00:00:00 EST 2023

KRAS and EGFR Mutations Differentially Alter ABC Drug Transporter Expression in Cisplatin-Resistant Non-Small Cell Lung Cancer.

International journal of molecular sciences

Jaromi L,Csongei V,Vesel M,Abdelwahab EMM,Soltani A,Torok Z,Smuk G,Sarosi V,Pongracz JE

MA5-12436 was used in Immunohistochemistry to confirm that, in lung AC-s, KRAS and EGFR driver mutations differentially affect both drug transporter expression and the cisplatin-induced WNT signalling microenvironment.

Thu May 20 00:00:00 EDT 2021

KRAS and EGFR Mutations Differentially Alter ABC Drug Transporter Expression in Cisplatin-Resistant Non-Small Cell Lung Cancer.

International journal of molecular sciences

Jaromi L,Csongei V,Vesel M,Abdelwahab EMM,Soltani A,Torok Z,Smuk G,Sarosi V,Pongracz JE

Thu May 20 00:00:00 EDT 2021

Contribution of TRESK two-pore domain potassium channel to bone cancer-induced spontaneous pain and evoked cutaneous pain in rats.

Molecular pain

Liu JP,Jing HB,Xi K,Zhang ZX,Jin ZR,Cai SQ,Tian Y,Cai J,Xing GG

MA5-12436 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Radioimmune assays to suggest that the differential distribution and decreased expression of TRESK in the periosteum and skin, which is attributed to the lack of IB4+ fibers innervation within the periosteum of the tibia, probably contribute to the behavioral divergence of cancer-induced spontaneous pain and evoked pain in bone cancer rats.

Thu Jan 13 00:00:00 EST 2022

Contribution of TRESK two-pore domain potassium channel to bone cancer-induced spontaneous pain and evoked cutaneous pain in rats.

Molecular pain

Liu JP,Jing HB,Xi K,Zhang ZX,Jin ZR,Cai SQ,Tian Y,Cai J,Xing GG

Thu Jan 13 00:00:00 EST 2022

Intra-Amniotic Infection with Ureaplasma parvum Causes Preterm Birth and Neonatal Mortality That Are Prevented by Treatment with Clarithromycin.

mBio

Motomura K,Romero R,Xu Y,Theis KR,Galaz J,Winters AD,Slutsky R,Garcia-Flores V,Zou C,Levenson D,Para R,Ahmad MM,Miller D,Hsu CD,Gomez-Lopez N

MA5-12436 was used in Immunocytochemistry-immunoflourescence to shed light on the maternal-fetal immunobiology of intra-amniotic infection.

Tue Jun 23 00:00:00 EDT 2020

ROCK2 inhibition triggers the collective invasion of colorectal adenocarcinomas.

The EMBO journal

Libanje F,Raingeaud J,Luan R,Thomas Z,Zajac O,Veiga J,Marisa L,Adam J,Boige V,Malka D,Goéré D,Hall A,Soazec JY,Prall F,Gelli M,Dartigues P,Jaulin F

MA5-12436 was used in Immunohistochemistry-immunofluorescence to identify FARP2 as the Rac1 GEF necessary for CRC collective invasion.

Mon Jul 15 00:00:00 EDT 2019

Human Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC).

Cellular and molecular gastroenterology and hepatology

Senger S,Ingano L,Freire R,Anselmo A,Zhu W,Sadreyev R,Walker WA,Fasano A

MA5-12436 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to study how to use human fetal organoids to gain insights into necrotising enterocolitis pathogenesis.

Mon Mar 21 00:00:00 EDT 2022

A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer.

Breast cancer research : BCR

Carter EP,Gopsill JA,Gomm JJ,Jones JL,Grose RP

MA5-12436 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to develop an in vitro, 3D model of the human breast duct for studying early events in breast cancer development.

Fri Apr 21 00:00:00 EDT 2017

A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer.

Breast cancer research : BCR

Carter EP,Gopsill JA,Gomm JJ,Jones JL,Grose RP

Fri Apr 21 00:00:00 EDT 2017

Differentiation of Human Induced Pluripotent Stem Cells to Mammary-like Organoids.

Stem cell reports

Qu Y,Han B,Gao B,Bose S,Gong Y,Wawrowsky K,Giuliano AE,Sareen D,Cui X

Published figure using EpCAM monoclonal antibody (Product # MA5-12436) in Immunocytochemistry

Tue Feb 14 00:00:00 EST 2017

Interpolation method for accurate affinity ranking of arrayed ligand-analyte interactions.

Analytical biochemistry

Schasfoort RB,Andree KC,van der Velde N,van der Kooi A,Stojanović I,Terstappen LW

MA5-12436 was used to study accurate affinity ranking of arrayed ligand-analyte interactions by an interpolation method

Sun May 01 00:00:00 EDT 2016

Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.

Anticancer research

Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK

MA5-12436 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells

Fri Nov 01 00:00:00 EDT 2013

Flow-regulated endothelial S1P receptor-1 signaling sustains vascular development.

Developmental cell

Jung B,Obinata H,Galvani S,Mendelson K,Ding BS,Skoura A,Kinzel B,Brinkmann V,Rafii S,Evans T,Hla T

Published figure using EpCAM monoclonal antibody (Product # MA5-12436) in Immunocytochemistry

Tue Sep 11 00:00:00 EDT 2012

Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.

Cytometry. Part B, Clinical cytometry

Eifler RL,Lind J,Falkenhagen D,Weber V,Fischer MB,Zeillinger R

MA5-12436 was used in immunocytochemistry to develop a process involving leukapheresis, elutriation and FACS to isolate circulating tumor cells from large volumes

Tue Mar 01 00:00:00 EST 2011

Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Bellone S,Siegel ER,Cocco E,Cargnelutti M,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Pecorelli S,Santin AD

MA5-12436 was used in immunohistochemistry to study the therapeutic implications of epithelial cell adhesion molecule overexpression in epithelial ovarian carcinomas

Wed Jul 01 00:00:00 EDT 2009

EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Neighbors M,Apt D,Chang JC,Brinkman A,Sipos-Solman I,Ong R,Leong S,Punnonen J

MA5-12436 was used in western blot to examine a novel costimulatory adjuvant for tumor immuno therapy

Mon Sep 01 00:00:00 EDT 2008

Inhibition of proliferation by PERK regulates mammary acinar morphogenesis and tumor formation.

PloS one

Sequeira SJ,Ranganathan AC,Adam AP,Iglesias BV,Farias EF,Aguirre-Ghiso JA

MA5-12436 was used in immunocytochemistry to study the role of adhesion-dependent regulation of PERK activity in modulating mammary acinar morphogenesis and breast tumorigenesis via inhibition of proliferation

Wed Jul 18 00:00:00 EDT 2007

Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.

European journal of immunology

Elia L,Mennuni C,Storto M,Podda S,Calvaruso F,Salucci V,Aurisicchio L,Scarito A,Ciliberto G,La Monica N,Palombo F

MA5-12436 was used in flow cytometry to evaluate the Ep-CAM genetic vaccination strategy in animal models

Mon May 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; lymphocyte antigen 74; Major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); panepithelial glycoprotein 314; Protein 289A; Trop-1 protein; Tumor-associated calcium signal transducer 1

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Gene Aliases: CD326; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; EpCAM1; ESA; GA733-2; gp40; HNPCC8; KS1/4; KSA; Ly74; M1S2; M4S1; MIC18; MK-1; Tacsd1; TACSTD1; TROP1

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UniProt ID: (Human) P16422, (Mouse) Q99JW5

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Entrez Gene ID: (Human) 4072, (Mouse) 17075

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EpCAM Monoclonal Antibody (323/A3)

EpCAM Antibody (MA5-12436) in ICC/IF

Product Details

MA5-12436

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Radioimmune Assays (RIA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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