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EphA1 (extracellular) Antibody (AER-011-F-50UL) in Flow
Cell surface detection ofEphA1by direct flow cytometry in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + EphA1 (extracellular)-FITC Antibody (#AER-011-F), (5 µg).
Product Details
AER-011-F-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1, Extracellular, N-terminus
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embryonic stem cell kinase; EPH tyrosine kinase; EPH tyrosine kinase 1; ephrin receptor EphA1; Ephrin type-A receptor 1; erythropoietin-producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; hEpha1; mEpha1; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH; Tyrosine-protein kinase receptor ESK
Gene Aliases: 5730453L17Rik; AL033318; EPH; EPHA1; EPHT; EPHT1; Esk
UniProt ID: (Human) P21709, (Mouse) Q60750
Entrez Gene ID: (Human) 2041, (Mouse) 13835, (Rat) 312279
protein kinase activity
transmembrane-ephrin receptor activity
ATP binding
protein kinase binding
nucleotide binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
angiogenesis
positive regulation of cell-matrix adhesion
negative regulation of protein kinase activity
cell surface receptor signaling pathway
positive regulation of cell proliferation
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
negative regulation of cell migration
substrate adhesion-dependent cell spreading
somatic stem cell population maintenance
regulation of GTPase activity
positive regulation of angiogenesis
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of stress fiber assembly
activation of GTPase activity
protein phosphorylation
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
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