Invitrogen

EphA4 Monoclonal Antibody (4C8H5)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

15 Published Figures

30 References

View all (29) EphA4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (15)
Advanced Verification (1)

EphA4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

EphA4 Antibody (37-1600) in ICC/IF

Molecular and cellular biology 2013 - Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Immunocytochemistry

EphA4 Antibody in Immunohistochemistry (IHC)

EphA4 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

EphA4 Antibody — Fig. 1 Reduction of EphA4 protein and mRNA levels in the hippocampus and cortex of APPPS1 mice. Representative images ( a ) and quantification ( b ) of a Western blot analysis of hippocampal EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot) with GAPDH protein levels as a loading control (unpaired t test and Mann-Whitney test respectively, n = 4-8 mice/group). Representative images ( c ) and quantification ( d ) of a Western blot analysis of cortical EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot). GAPDH protein levels were assessed to control for equal loading (unpaired t test, n = 4 mice/group). e Representative images of RNA scope with specific probes for EphA4 in the DG, CA3, and CA1 regions of the hippocampus of Ctrl and EphA4-KO mice. Hoechst was used to stain cell nuclei. * p <= 0.05, ** p <= 0.01. Scale bar = 100 mum. Abbreviation: Ctrl control {KO}

Product Details

37-1600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 22 publications 22 publications

Immunohistochemistry (IHC)

3-10 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

5 µg

View 3 publications 3 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4C8H5

Immunogen

A synthetic peptide derived from the C-terminus human EphA4 receptor protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533301

Product Specific Information

This antibody is specific for the endogenous EphA4 receptor protein. No cross-reactivity with EphA5 or EphA7 has been detected. On western blots, this antibody identifies a band at ~110-120 kDa. Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by western blot and immunoprecipitation and on transfected 293 cells and mouse hippocampus by immunohistochemistry.

Target Information

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal miR-532-5p induced by long-term exercise rescues blood-brain barrier function in 5XFAD mice via downregulation of EPHA4.

Aging cell

Liang X,Fa W,Wang N,Peng Y,Liu C,Zhu M,Tian N,Wang Y,Han X,Qiu C,Hou T,Du Y

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Sun Jan 01 00:00:00 EST 2023

EphA4 targeting agents protect motor neurons from cell death induced by amyotrophic lateral sclerosis -astrocytes.

iScience

Dennys C,Baggio C,Rodrigo R,Roussel F,Kulinich A,Heintzman S,Fox A,Kolb SJ,Shaw PJ,Ethell IM,Pellecchia M,Meyer KC

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Fri Sep 16 00:00:00 EDT 2022

EphA4 targeting agents protect motor neurons from cell death induced by amyotrophic lateral sclerosis -astrocytes.

iScience

Dennys C,Baggio C,Rodrigo R,Roussel F,Kulinich A,Heintzman S,Fox A,Kolb SJ,Shaw PJ,Ethell IM,Pellecchia M,Meyer KC

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Fri Sep 16 00:00:00 EDT 2022

SIPA1L1/SPAR1 Interacts with the Neurabin Family of Proteins and is Involved in GPCR Signaling.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Matsuura K,Kobayashi S,Konno K,Yamasaki M,Horiuchi T,Senda T,Hayashi T,Satoh K,Arima-Yoshida F,Iwasaki K,Negishi L,Yasui-Shimizu N,Kohu K,Kawahara S,Kirino Y,Nakamura T,Watanabe M,Yamamoto T,Manabe T,Akiyama T

37-1600 was used in Western Blotting to perform expression analyses using super-resolution microscopy (SRM) in mouse brain and demonstrated that SIPA1L1 is mainly localized to general submembranous regions in neurons, but surprisingly, not to PSD. Our screening for physiological interactors of SIPA1L1 in mouse brain identified spinophilin and neurabin-1, regulators of G-protein-coupled receptor (GPCR) signaling, but rejected PSD-95/NMDA-R-complex components.

Wed Mar 23 00:00:00 EDT 2022

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Cell chemical biology

Wolff DW,Deng Z,Bianchi-Smiraglia A,Foley CE,Han Z,Wang X,Shen S,Rosenberg MM,Moparthy S,Yun DH,Chen J,Baker BK,Roll MV,Magiera AJ,Li J,Hurley E,Feltri ML,Cox AO,Lee J,Furdui CM,Liu L,Bshara W,LaConte LEW,Kandel ES,Pasquale EB,Qu J,Hedstrom L,Nikiforov MA

37-1600 was used in Western Blotting to demonstrate that GMPR plays a key role in EPHA4-mediated RAC1 suppression.

Thu Jun 16 00:00:00 EDT 2022

NMR-Guided Design of Potent and Selective EphA4 Agonistic Ligands.

Journal of medicinal chemistry

Baggio C,Kulinich A,Dennys CN,Rodrigo R,Meyer K,Ethell I,Pellecchia M

37-1600 was used in Western Blotting to show that the data form a strong foundation for the translation of these agents for the treatment of ALS and potentially other human diseases.

Thu Aug 12 00:00:00 EDT 2021

Environmental enrichment during the chronic phase after experimental stroke promotes functional recovery without synergistic effects of EphA4 targeted therapy.

Human molecular genetics

de Boer A,Storm A,Gomez-Soler M,Smolders S,Rué L,Poppe L,B Pasquale E,Robberecht W,Lemmens R

37-1600 was used in Western Blotting to assess the effectiveness of a basic enriched environment in the chronic phase after photothrombotic stroke in mice as well as the therapeutic potential of EphA4 targeted therapy followed by rehabilitation.

Fri Mar 13 00:00:00 EDT 2020

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

Acta neuropathologica

Garcia-Diaz B,Bachelin C,Coulpier F,Gerschenfeld G,Deboux C,Zujovic V,Charnay P,Topilko P,Baron-Van Evercooren A

37-1600 was used in Western Blotting to study schwann cell migratory behaviour ex vivo and in vivo after exogenous transplantation in the demyelinated spinal cord.

Sun Sep 01 00:00:00 EDT 2019

EphA4 loss improves social memory performance and alters dendritic spine morphology without changes in amyloid pathology in a mouse model of Alzheimer's disease.

Alzheimer's research & therapy

Poppe L,Rué L,Timmers M,Lenaerts A,Storm A,Callaerts-Vegh Z,Courtand G,de Boer A,Smolders S,Van Damme P,Van Den Bosch L,D'Hooge R,De Strooper B,Robberecht W,Lemmens R

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Thu Dec 12 00:00:00 EST 2019

Lowering EphA4 Does Not Ameliorate Disease in a Mouse Model for Severe Spinal Muscular Atrophy.

Frontiers in neuroscience

Poppe L,Smolders S,Rué L,Timmers M,Lenaerts A,Storm A,Schoonaert L,de Boer A,Van Damme P,Van Den Bosch L,Robberecht W,Lemmens R

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Fri Oct 20 00:00:00 EDT 2023

Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage.

Journal of neuroinflammation

Kowalski EA,Chen J,Hazy A,Fritsch LE,Gudenschwager-Basso EK,Chen M,Wang X,Qian Y,Zhou M,Byerly M,Pickrell AM,Matson JB,Allen IC,Theus MH

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Mon Nov 11 00:00:00 EST 2019

Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage.

Journal of neuroinflammation

Kowalski EA,Chen J,Hazy A,Fritsch LE,Gudenschwager-Basso EK,Chen M,Wang X,Qian Y,Zhou M,Byerly M,Pickrell AM,Matson JB,Allen IC,Theus MH

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Mon Nov 11 00:00:00 EST 2019

Reducing EphA4 before disease onset does not affect survival in a mouse model of Amyotrophic Lateral Sclerosis.

Scientific reports

Rué L,Timmers M,Lenaerts A,Smolders S,Poppe L,de Boer A,Van Den Bosch L,Van Damme P,Robberecht W,Lemmens R

37-1600 was used in Western Blotting to gain more insights in the cell-specific role of decreasing EphA4 expression in addition to timing and amount of EphA4 reduction.

Tue Oct 01 00:00:00 EDT 2019

Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virus.

Nature microbiology

Chen J,Sathiyamoorthy K,Zhang X,Schaller S,Perez White BE,Jardetzky TS,Longnecker R

37-1600 was used in Blocking experiments to discover EphA2 as an Epstein-Barr virus epithelial cell receptor has important implications for EBV pathogenesis and may uncover new potential targets that can be used for the development of novel intervention strategies.

Thu Feb 01 00:00:00 EST 2018

An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation.

Development (Cambridge, England)

Rothe M,Kanwal N,Dietmann P,Seigfried FA,Hempel A,Schütz D,Reim D,Engels R,Linnemann A,Schmeisser MJ,Bockmann J,Kühl M,Boeckers TM,Kühl SJ

371600 was used in western blot to show a direct interaction between Sipa1l3 and Epha4 is required for proper eye development

Sun Jan 15 00:00:00 EST 2017

Potent and Selective EphA4 Agonists for the Treatment of ALS.

Cell chemical biology

Wu B,De SK,Kulinich A,Salem AF,Koeppen J,Wang R,Barile E,Wang S,Zhang D,Ethell I,Pellecchia M

37-1600 was used in Western Blotting to report on the design and characterization of a family of EphA4 targeting agents that bind to its ligand binding domain with nanomolar affinity.

Thu Mar 16 00:00:00 EDT 2017

Eph receptor interclass cooperation is required for the regulation of cell proliferation.

Experimental cell research

Jurek A,Genander M,Kundu P,Catchpole T,He X,Strååt K,Sabelström H,Xu NJ,Pettersson S,Henkemeyer M,Frisén J

371600 was used in immunoprecipitation and western blot to describe the contributions of ephrin-B1 and ephrin-B2 to the migration and proliferation of the intestinal stem cell niche

Sat Oct 15 00:00:00 EDT 2016

The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve.

The European journal of neuroscience

Joly S,Jordi N,Schwab ME,Pernet V

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Wed Oct 01 00:00:00 EDT 2014

Neuropathological and biochemical assessments of an Alzheimer's disease patient treated with the γ-secretase inhibitor semagacestat.

American journal of neurodegenerative disease

Roher AE,Maarouf CL,Kokjohn TA,Whiteside CM,Kalback WM,Serrano G,Belden C,Liebsack C,Jacobson SA,Sabbagh MN,Beach TG

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Wed Jan 28 00:00:00 EST 2015

Modifying expression of EphA4 and its downstream targets improves functional recovery after stroke.

Human molecular genetics

Lemmens R,Jaspers T,Robberecht W,Thijs VN

37-1600 was used in western blot to test if EphA4 affects stroke recovery and evaluate Eph44 as a therapeutic target.

Sat Jun 01 00:00:00 EDT 2013

Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors.

Molecular and cellular biology

Semerdjieva S,Abdul-Razak HH,Salim SS,Yáñez-Muñoz RJ,Chen PE,Tarabykin V,Alifragis P

37-1600 was used in immunohistochemistry to investigate the role of Src-like adaptor protein in the context of NMDA receptor signaling

Mon Apr 01 00:00:00 EDT 2013

Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors.

Molecular and cellular biology

Semerdjieva S,Abdul-Razak HH,Salim SS,Yáñez-Muñoz RJ,Chen PE,Tarabykin V,Alifragis P

37-1600 was used in immunohistochemistry to investigate the role of Src-like adaptor protein in the context of NMDA receptor signaling

Mon Apr 01 00:00:00 EDT 2013

Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration.

The European journal of neuroscience

Kempf A,Montani L,Petrinovic MM,Schroeter A,Weinmann O,Patrignani A,Schwab ME

37-1600 was used in western blot to study the increased levels of certain axon guidance proteins in the CNS of Nogo-A ablated mice and their role in limiting the outgrowth of neurites in the absence of Nogo.

Sun Dec 01 00:00:00 EST 2013

EPHA4 is a disease modifier of amyotrophic lateral sclerosis in animal models and in humans.

Nature medicine

Van Hoecke A,Schoonaert L,Lemmens R,Timmers M,Staats KA,Laird AS,Peeters E,Philips T,Goris A,Dubois B,Andersen PM,Al-Chalabi A,Thijs V,Turnley AM,van Vught PW,Veldink JH,Hardiman O,Van Den Bosch L,Gonzalez-Perez P,Van Damme P,Brown RH,van den Berg LH,Robberecht W

37-1600 was used in western blot to elucidate the role of Epha4 in models of amyotrophic lateral sclerosis.

Sat Sep 01 00:00:00 EDT 2012

Profiling Eph receptor expression in cells and tissues: a targeted mass spectrometry approach.

Cell adhesion & migration

Noberini R,Rubio de la Torre E,Pasquale EB

Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Western Blot

Wed Nov 28 00:00:00 EST 2012

Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.

PloS one

Yumoto N,Wakatsuki S,Kurisaki T,Hara Y,Osumi N,Frisén J,Sehara-Fujisawa A

37-1600 was used in immunoprecipitation to test if ADAM family proteins contribute to the development of the neuromuscular junction

Thu Oct 02 00:00:00 EDT 2008

alpha2-Chimaerin is an essential EphA4 effector in the assembly of neuronal locomotor circuits.

Neuron

Beg AA,Sommer JE,Martin JH,Scheiffele P

37-1600 was used in western blot to examine neuronal RacGAP alpha2-chimaerin as an effector for the receptor tyrosine kinase EphA4 in cortical and spinal motor circuits

Thu Sep 06 00:00:00 EDT 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Richter M,Murai KK,Bourgin C,Pak DT,Pasquale EB

Wed Dec 19 00:00:00 EST 2007

Bioinformatics

Protein Aliases: Cek8; EK8; epa4; EPH-like kinase 8; Ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1

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Gene Aliases: 2900005C20Rik; AI385584; CEK8; EPHA4; HEK8; rb; SEK; Sek1; TYRO1

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UniProt ID: (Chicken) Q07496, (Human) P54764, (Mouse) Q03137

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Entrez Gene ID: (Chicken) 395559, (Human) 2043, (Mouse) 13838

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Function(s)

protein kinase activity transmembrane receptor protein tyrosine kinase activity GPI-linked ephrin receptor activity transmembrane-ephrin receptor activity ATP binding PH domain binding identical protein binding DH domain binding protein tyrosine kinase binding protein binding ephrin receptor binding nucleotide binding protein tyrosine kinase activity ephrin receptor activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

somitogenesis cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development axon guidance adult walking behavior motor neuron axon guidance peptidyl-tyrosine phosphorylation corticospinal tract morphogenesis positive regulation of kinase activity adherens junction organization segmentation regulation of GTPase activity positive regulation of JUN kinase activity protein autophosphorylation ephrin receptor signaling pathway negative regulation of axon regeneration regulation of astrocyte differentiation regulation of axonogenesis protein stabilization regulation of dendritic spine morphogenesis positive regulation of protein tyrosine kinase activity nephric duct morphogenesis cochlea development fasciculation of sensory neuron axon fasciculation of motor neuron axon synapse pruning negative regulation of long-term synaptic potentiation positive regulation of beta-amyloid formation positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process negative regulation of proteolysis involved in cellular protein catabolic process cellular response to beta-amyloid regulation of modification of synaptic structure positive regulation of Rho guanyl-nucleotide exchange factor activity glial cell migration positive regulation of dendrite morphogenesis protein phosphorylation nervous system development phosphorylation

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EphA4 Monoclonal Antibody (4C8H5)

EphA4 Antibody (37-1600) in ICC/IF

Product Details

37-1600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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