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EphB2 Antibody (PA5-97252) in WB
Western blot analysis of EphB2 in extracts of various cell lines. Samples were incubated with EphB2 Polyclonal antibody (Product # PA5-97252) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-97252
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-540 of human EPHB2 (NP_0591452)
Conjugate
Form
Liquid
Concentration
1.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809054
Product Specific Information
Immunogen sequence: PIGRCMCKAG FEAVENGTVC RGCPSGTFKA NQGDEACTHC PINSRTTSEG ATNCVCRNGY YRADLDPLDM PCTTIPSAPQ AVISSVNETS LMLEWTPPRD SGGREDLVYN IICKSCGSGR GACTRCGDNV QYAPRQLGLT EPRIYISDLL AHTQYTFEIQ AVNGVTDQSP FSPQFASVNI TTNQAAPSAV SIMHQVSRTV DSITLSWSQP DQPNGVILDY ELQYYEKELS EYNATAIKSP TNTVTVQGLK AGAIYVFQVR ARTVAGYGRY SGKMYFQTMT EAEYQTSIQE K; Positive Samples: NCI-H460, HepG2, HeLa, HT-1080, SW620, Mouse brain, Mouse heart; Cellular Location: Cell membrane, Cell projection, Single-pass type I membrane protein, axon, dendrite
Target Information
EPHB2 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Developmentally-regulated Eph-related tyrosine kinase; EFL6; EFNB3; EK5; ELK-related tyrosine kinase; EPH B2; EPH tyrosine kinase 3; EPH-like kinase 5; EPHB3; Ephrin B1; Ephrin B2; Ephrin B3; Ephrin B4; Ephrin type-B receptor 2; EphrinB1; EphrinB2; EphrinB3; EphrinB4; EPLG8; ETK2; HEK-6; HEK2; LERK8; MGC87492; Neural kinase; Nuk receptor tyrosine kinase; protein-tyrosine kinase HEK5; Renal carcinoma antigen NY-REN-47; TYRO6; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase receptor SEK-3; Tyrosine-protein kinase TYRO5
Gene Aliases: CAPB; Cek5; DRT; EK5; EPHB2; EPHT3; EPTH3; ERK; ETECK; HEK5; Nuk; PCBC; Prkm5; Qek5; Sek3; TYRO5
UniProt ID: (Human) P29323, (Mouse) Q6GTQ7
Entrez Gene ID: (Human) 2048, (Mouse) 13844
protein tyrosine kinase activity
transmembrane-ephrin receptor activity
receptor binding
protein binding
ATP binding
axon guidance receptor activity
identical protein binding
nucleotide binding
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor activity
ephrin receptor activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
angiogenesis
urogenital system development
nervous system development
axon guidance
axonal fasciculation
learning
phosphorylation
peptidyl-tyrosine phosphorylation
optic nerve morphogenesis
central nervous system projection neuron axonogenesis
corpus callosum development
retinal ganglion cell axon guidance
inner ear morphogenesis
ephrin receptor signaling pathway
positive regulation of long-term neuronal synaptic plasticity
camera-type eye morphogenesis
negative regulation of axonogenesis
regulation of body fluid levels
positive regulation of synapse assembly
palate development
dendritic spine development
dendritic spine morphogenesis
commissural neuron axon guidance
cell morphogenesis
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
organ morphogenesis
regulation of neuronal synaptic plasticity
regulation of axonogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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