Invitrogen

EphB2 Monoclonal Antibody (1A6C9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

16 References

View all (24) EphB2 antibodies

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Datasheet

Protocols

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FIGURE: 1 / 17

EphB2 Antibody (37-1700) in WB

Western blot was performed using Anti-EphB2 Monoclonal Antibody (1A6C9) (Product # 37-1700) and 117, 110kDa bands corresponding to EphB2 was increased upon VEGF treatment in HUVEC. Also, the expression was highest in COLO 205 in comparison to SW480 and HT-29 as reported. Membrane enriched cell extracts (30 ug lysate) of HUVEC (Lane 1), HUVEC treated with VEGF (20ng/ml, 10 min) (Lane 2), COLO 205 (Lane 3), SW480 (Lane 4) and HT-29 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins... View More

EphB2 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

EphB2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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EphB2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of EphB2 was observed in COLO 205 in comparison to SW480 and HT-29 using EphB2 Monoclonal Antibody (1A6C9) (Product # 37-1700) in western blot. {RE}

Product Details

37-1700

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1A6C9

Immunogen

A synthetic peptide derived from the C-terminal region of the recombinant EphB2 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533302

Target Information

EPHB2 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A T cell redirection platform for co-targeting dual antigens on solid tumors.

mAbs

Enderle L,Shalaby KH,Gorelik M,Weiss A,Blazer LL,Paduch M,Cardarelli L,Kossiakoff A,Adams JJ,Sidhu SS

37-1700 was used in Western Blot to engineer the Dual Antigen T cell Engager to enable T cell activity to specific solid cancer cell features.

Tue Dec 28 00:00:00 EST 2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

37-1700 was used in Western Blotting to identify a critical role for EphB2 in the ENS and reveal a unique EphB2-mediated molecular program of synapse regulation in enteric neurons.

Mon Nov 01 00:00:00 EDT 2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

37-1700 was used in Western Blotting to identify a critical role for EphB2 in the ENS and reveal a unique EphB2-mediated molecular program of synapse regulation in enteric neurons.

Mon Nov 01 00:00:00 EDT 2021

Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility.

Nature communications

Washburn HR,Xia NL,Zhou W,Mao YT,Dalva MB

37-1700 was used in Western Blot to demonstrate that the positive surface charge of the hinge region of the N-terminal domain in the GluN1 subunit of the NMDAR is required to maintain NMDARs at dendritic spine synapses and mediates the direct extracellular interaction with a negatively charged phospho-tyrosine on the receptor tyrosine kinase EphB2.

Wed Jan 29 00:00:00 EST 2020

Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation.

Experimental cell research

Wu Z,Ashlin TG,Xu Q,Wilkinson DG

37-1700 was used in Western Blot to propose that although polarised forward signaling is the principal driver of segregation, reverse signaling enables bidirectional repulsion which prevents mingling of each population into the other.

Thu Aug 01 00:00:00 EDT 2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

eLife

Koppers M,Cagnetta R,Shigeoka T,Wunderlich LC,Vallejo-Ramirez P,Qiaojin Lin J,Zhao S,Jakobs MA,Dwivedy A,Minett MS,Bellon A,Kaminski CF,Harris WA,Flanagan JG,Holt CE

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Immunoprecipitation

Wed Nov 20 00:00:00 EST 2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

eLife

Koppers M,Cagnetta R,Shigeoka T,Wunderlich LC,Vallejo-Ramirez P,Qiaojin Lin J,Zhao S,Jakobs MA,Dwivedy A,Minett MS,Bellon A,Kaminski CF,Harris WA,Flanagan JG,Holt CE

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Immunoprecipitation

Wed Nov 20 00:00:00 EST 2019

Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.

Molecular biology of the cell

Okumura F,Joo-Okumura A,Obara K,Petersen A,Nishikimi A,Fukui Y,Nakatsukasa K,Kamura T

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Western Blot

Wed Nov 15 00:00:00 EST 2017

YES oncogenic activity is specified by its SH4 domain and regulates RAS/MAPK signaling in colon carcinoma cells.

American journal of cancer research

Dubois F,Leroy C,Simon V,Benistant C,Roche S

37-1700 was used in immunoprecipitation to elucidate how YES contributes to colorectal cancer progression

Thu Aug 13 00:00:00 EDT 2015

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.