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Ephrin A2 Antibody (PA5-47806) in ICC/IF
Immunocytochemistry analysis of Ephrin A2 in immersion fixed rat hippocampal neurons. Samples were incubated in Ephrin A2 polyclonal antibody (Product # PA5-47806) using a dilution of 10 µg/mL for 3 hours at room temperature. Neurons were stained (green) and glial cells were labeled by using anti-GFAP antibodies (red).
Product Details
PA5-47806
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A2 Met1-Asn184
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608569
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, recombinant human (rh) Ephrin-A3, rmEphrin-A4, rhEphrin-A5, rmEphrin-B1, rmEphrin-B2, and rhEphrin-B3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1(Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3(Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, Ephrin-A2 (Elf-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CEK7-L; CEK7-ligand; ELF-1; EPH related receptor tyrosine kinase ligand 6; EPH-related receptor tyrosine kinase ligand 6; ephrin A6; Ephrin-A2; EphrinA2; LERK-6
Gene Aliases: CEK7L; Efna2; Elf1; Epl6; Eplg6; Lerk6
UniProt ID: (Mouse) P52801
Entrez Gene ID: (Mouse) 13637
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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