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Epigen Antibody (PA5-79208) in WB
Western blot analysis of Epigen in Lane 1: rat PC-12 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Epigen polyclonal antibody (Product # PA5-79208) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondar... View More
Product Details
PA5-79208
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Epigen recombinant protein (Position: A19-K109).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746324
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. It is produced in several tissues, including the testis, liver, and heart, as well as in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EPG; Epigen; Epithelial mitogen
Gene Aliases: 2310069M11Rik; Epgn; epigen; RGD1560084
UniProt ID: (Mouse) Q924X1
Entrez Gene ID: (Rat) 289515, (Mouse) 71920
MAPK cascade
activation of MAPK activity
angiogenesis
epidermal growth factor receptor signaling pathway
biological_process
positive regulation of cell proliferation
positive regulation of MAP kinase activity
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
positive regulation of epithelial cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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