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Epo Antibody (500-P318BT-25UG) in ELISA
Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of Epo Polyclonal Antibody, Biotin (Product # 500-P318BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Epo Polyclonal Antibody (Product # 500-P318-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human EPO.
Product Details
500-P318BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
CHO cells-derived Recombinant Human EPO
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930369
Product Specific Information
AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EPO (500-P318) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human EPO.
Western Blot: To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P318BT-1MG will be provided as 2 x 500 µg
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epoetin; Erythropoietin; MGC138142
Gene Aliases: EP; EPO; MVCD2
UniProt ID: (Human) P01588
Entrez Gene ID: (Human) 2056
negative regulation of transcription from RNA polymerase II promoter
apoptotic process
acute-phase response
signal transduction
embryo implantation
aging
blood circulation
positive regulation of cell proliferation
response to salt stress
negative regulation of calcium ion transport into cytosol
positive regulation of neuron projection development
peptidyl-serine phosphorylation
erythrocyte differentiation
activation of protein kinase activity
response to lipopolysaccharide
response to vitamin A
response to testosterone
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
hemoglobin biosynthetic process
erythrocyte maturation
response to estrogen
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of Ras protein signal transduction
response to axon injury
response to electrical stimulus
response to hyperoxia
regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of ERK1 and ERK2 cascade
response to interleukin-1
cellular hyperosmotic response
negative regulation of neuron death
negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
negative regulation of erythrocyte apoptotic process
negative regulation of cation channel activity
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