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Epo Antibody (MA5-23874) in Neu
Neutralization of Epo in TF‚1 human erythroleukemic cell line. Samples were incubated in Epo monoclonal antibody (Product # MA5-23874). Recombinant Mouse Erythropoietin/EPO stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody. The ND50 is typically 0.15-0.75 µg/mL.
Product Details
MA5-23874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
148436
Immunogen
CHO-derived recombinant mouse Erythropoietin Ala27-Arg192
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609655
Product Specific Information
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Erythropoietin; MGC138142
Gene Aliases: Epo
UniProt ID: (Mouse) P07321
Entrez Gene ID: (Mouse) 13856
angiogenesis
response to hypoxia
heart morphogenesis
regulation of transcription from RNA polymerase II promoter
apoptotic process
embryo implantation
positive regulation of cell proliferation
negative regulation of calcium ion transport into cytosol
peptidyl-serine phosphorylation
erythrocyte differentiation
negative regulation of myeloid cell apoptotic process
positive regulation of tyrosine phosphorylation of Stat5 protein
hemoglobin biosynthetic process
negative regulation of apoptotic process
erythrocyte maturation
positive regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of Ras protein signal transduction
cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
visceral serous pericardium development
cellular hyperosmotic response
negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
negative regulation of erythrocyte apoptotic process
negative regulation of cation channel activity
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