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EpoR Antibody (PA5-95480) in WB
Western blot analysis of EpoR in Lane 1: rat lung tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with EpoR polyclonal antibody (Product # PA5-95480) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Product Details
PA5-95480
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human EPO Receptor recombinant protein (Position: E48-E226).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2807282
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
EpoR encodes the erythropoietin receptor, which belongs to the cytokine receptor family. When erythropoietin binds to this receptor, it activates the Jak2 tyrosine kinase, which in turn activates various intracellular pathways including Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors. The stimulated erythropoietin receptor plays a role in the survival of erythroid cells. Defects in this gene may result in erythroleukemia and familial erythrocytosis. Dysregulation of this gene may also affect the growth of certain tumors. Multiple transcript variants are produced as a result of alternate splicing. Diseases associated with EPOR include Erythrocytosis, Familial, 1 and Erythroleukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EPO R; EPO-R; Erythropoietin receptor; pEpoR
Gene Aliases: EPO-R; EPOR
UniProt ID: (Human) P19235, (Mouse) P14753
Entrez Gene ID: (Human) 2057, (Mouse) 13857, (Rat) 24336
signal transduction
positive regulation of cytosolic calcium ion concentration
brain development
heart development
positive regulation of cell proliferation
positive regulation of neuron projection development
erythropoietin-mediated signaling pathway
negative regulation of neuron apoptotic process
decidualization
positive regulation of ERK1 and ERK2 cascade
negative regulation of hydrogen peroxide-induced cell death
heart morphogenesis
embryo development
viral process
cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
visceral serous pericardium development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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