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EpoR Antibody (MA5-29248) in WB
Western Blot was performed using Anti-EpoR Recombinant Rabbit Monoclonal Antibody (16) (Product # MA5-29248). In Fig.a, a 60 kDa band corresponding to Erythropoietin receptor was observed across cell lines tested except MCF-7 and SK-OV3 which is reported to be low. In Fig.b, a 95 kDa band corresponding to Erythropoietin receptor was upregulated during 5 days of HSC to erythroid differentiation and gradually downregulated in further differentiation process which is expected. In Fig.a, membrane enriched extracts (30 µg lysate) of K-562 (Lane... View More
Product Details
MA5-29248
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
016
Immunogen
Recombinant Human EPOR/Erythropoietin Receptor protein (Met1-Pro250)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785148
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human EPOR/Erythropoietin Receptor.
Target Information
EpoR encodes the erythropoietin receptor, which belongs to the cytokine receptor family. When erythropoietin binds to this receptor, it activates the Jak2 tyrosine kinase, which in turn activates various intracellular pathways including Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors. The stimulated erythropoietin receptor plays a role in the survival of erythroid cells. Defects in this gene may result in erythroleukemia and familial erythrocytosis. Dysregulation of this gene may also affect the growth of certain tumors. Multiple transcript variants are produced as a result of alternate splicing. Diseases associated with EPOR include Erythrocytosis, Familial, 1 and Erythroleukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EPO R; EPO-R; Erythropoietin receptor; pEpoR
Gene Aliases: EPO-R; EPOR
UniProt ID: (Human) P19235
Entrez Gene ID: (Human) 2057
signal transduction
positive regulation of cytosolic calcium ion concentration
brain development
heart development
positive regulation of cell proliferation
positive regulation of neuron projection development
erythropoietin-mediated signaling pathway
negative regulation of neuron apoptotic process
decidualization
positive regulation of ERK1 and ERK2 cascade
negative regulation of hydrogen peroxide-induced cell death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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