Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (3B5), Biotin

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

52 References

View all (273) Her-2 (ErbB2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (1)

ErbB2 (HER-2) Antibody in Western Blot (WB)

FIGURE: 1 / 5

ErbB2 (HER-2) Antibody (MA5-13672) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (1) and MDA-MB-231 (2). The blots were probed with Anti-ErbB2 Mouse Monoclonal Antibody (Product # MA5-13672, 1 µg/mL dilution) and detected by chemiluminescence using Streptavidin-HRP (Product # N200, 1:10,000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 but not in MDA-MB-231, which is a negative control for ErbB2. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # ... View More

ErbB2 (HER-2) Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

MA5-13672

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

1:1,000-1:1,500

View 26 publications 26 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

ELISA (ELISA)

View 3 publications 3 publications

Immunoprecipitation (IP)

1:25

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Avian, Cat, Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

A synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.

View immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982527

Product Specific Information

MA5-13672 targets HER-2 in IHC, IP, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-13672 immunogen is a synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impedance spectroscopy assisted by magnetic nanoparticles as a potential biosensor principle for breast cancer cells in suspension.

Physiological measurement

Silva JG,Cárdenas RA,Quiróz AR,Sánchez V,Lozano LM,Pérez NM,López J,Villanueva C,González CA

MA5-13672 was used in ELISA to study the use of impedence spectroscopy combined with magnetic nanoparticles for the measurement of beast cancer cells in suspension

Sun Jun 01 00:00:00 EDT 2014

Monitoring afatinib treatment in HER2-positive gastric cancer with 18F-FDG and 89Zr-trastuzumab PET.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Janjigian YY,Viola-Villegas N,Holland JP,Divilov V,Carlin SD,Gomes-DaGama EM,Chiosis G,Carbonetti G,de Stanchina E,Lewis JS

MA5-13672 was used in western blot to study the pharmacodynamics of afatinib in HER2-positive gastric cancer using PET

Sat Jun 01 00:00:00 EDT 2013

A cell-based screening system for influenza A viral RNA transcription/replication inhibitors.

Scientific reports

Ozawa M,Shimojima M,Goto H,Watanabe S,Hatta Y,Kiso M,Furuta Y,Horimoto T,Peters NR,Hoffmann FM,Kawaoka Y

MA5-13672 was used in western blot to develop a cell-based screening system to identify compounds that inhibit influenza A viral RNA transcription/replication

Mon Sep 30 00:00:00 EDT 2013

HER2/neu gene amplification and protein overexpression in gastric and gastroesophageal junction adenocarcinoma: a review of histopathology, diagnostic testing, and clinical implications.

Archives of pathology & laboratory medicine

Hechtman JF,Polydorides AD

MA5-13672 was used in immunohistochemistry to review the literature on HER2/neu gene amplification and protein overexpression in gastric and gastroesophageal junction adenocarcinoma

Fri Jun 01 00:00:00 EDT 2012

EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway.

Carcinogenesis

Cortés MA,Cariaga-Martinez AE,Lobo MV,Martín Orozco RM,Motiño O,Rodríguez-Ubreva FJ,Angulo J,López-Ruiz P,Colás B

MA5-13672 was used in immunocytochemistry, immunohistochemistry, and western blot to study the mechanism by which EGF promotes neuroendocrine-like differention of prostate cancer cells in the presence of a PI3 kinase inhibitor

Fri Jun 01 00:00:00 EDT 2012

Clinicopathologic features of the mixed epithelial and mesenchymal type metaplastic breast carcinoma with myoepithelial differentiation in a subset of six cases.

Pathology, research and practice

Yigit S,Pehlivan FS,Evcim G,Etit D

MA5-13672 was used in immunohistochemistry to study six cases of mixed epithelial and mesenchymal type metaplastic breast carcinoma with myoepithelial differentiation

Thu Mar 15 00:00:00 EDT 2012

Stromal caveolin-1 expression in breast carcinoma. Correlation with early tumor recurrence and clinical outcome.

Pathology oncology research : POR

El-Gendi SM,Mostafa MF,El-Gendi AM

MA5-13672 was used in immunohistochemistry to study the prognostic value of stromal caveolin-1 in breast cancer

Sun Apr 01 00:00:00 EDT 2012

Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action.

Breast cancer research : BCR

DeFazio-Eli L,Strommen K,Dao-Pick T,Parry G,Goodman L,Winslow J

MA5-13672 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors

Fri Apr 15 00:00:00 EDT 2011

Protective effects of prepubertal genistein exposure on mammary tumorigenesis are dependent on BRCA1 expression.

Cancer prevention research (Philadelphia, Pa.)

de Assis S,Warri A,Benitez C,Helferich W,Hilakivi-Clarke L

MA5-13672 was used in western blot to study the role of BRCA-1 in the protective effects of prepubertal genistein against mammary tumorigenesis

Thu Sep 01 00:00:00 EDT 2011

Recurrence of benign meningiomas: predictive value of proliferative index, BCL2, p53, hormonal receptors and HER2 expression.

British journal of neurosurgery

Abdelzaher E,El-Gendi SM,Yehya A,Gowil AG

MA5-13672 was used in immunohistochemistry to identify biomarkers that predict the recurrence of benign meningiomas

Thu Dec 01 00:00:00 EST 2011

Detection of minimal residual disease in blood and bone marrow in early stage breast cancer.

Cancer

Krishnamurthy S,Cristofanilli M,Singh B,Reuben J,Gao H,Cohen EN,Andreopoulou E,Hall CS,Lodhi A,Jackson S,Lucci A

MA5-13672 was used in immunohistochemistry to study the presence of circulating tumor cells in blood and disseminated tumor cells in bone marrow of women with early stage breast cancer

Thu Jul 15 00:00:00 EDT 2010

Glioma cells display complex cell surface topographies that resist the actions of cytolytic effector lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Hoa N,Ge L,Kuznetsov Y,McPherson A,Cornforth AN,Pham JT,Myers MP,Ahmed N,Salsman VS,Lamb LS,Bowersock JE,Hu Y,Zhou YH,Jadus MR

MA5-13672 was used in immunocytochemistry to study the mechanisms by which glioma cells evade the actions of cytolytic effector lymphocytes

Fri Oct 15 00:00:00 EDT 2010

Comparison of central HER2 testing with quantitative total HER2 expression and HER2 homodimer measurements using a novel proximity-based assay.

American journal of clinical pathology

Huang W,Reinholz M,Weidler J,Yolanda L,Paquet A,Whitcomb J,Lingle W,Jenkins RB,Chen B,Larson JS,Tan Y,Sherwood T,Bates M,Perez EA

MA5-13672 was used in ELISA to develop and evaluate a novel proximity-based assay for quantitating total HER2 expression and HER2 homodimer

Sun Aug 01 00:00:00 EDT 2010

Protein expression profiling of primary mammary epithelial cells derived from MMTV-neu mice revealed that HER2/NEU-driven changes in protein expression are functionally clustered.

IUBMB life

Park S,Lee KM,Ju JH,Kim J,Noh DY,Lee T,Shin I

MA5-13672 was used in western blot to profile protein expression in primary mammary epithelial cells derived from MMTV-neu mice to identify novel biomarkers of tumorigenesis

Fri Jan 01 00:00:00 EST 2010

Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus.

Journal of oncology

Rygiel AM,Milano F,Ten Kate FJ,Bergman JJ,Krishnadath KK

MA5-13672 was used in immunohistochemistry to study the correlation of Her-2 locus amplification with Her-2 protein overexpression in patients with Barrett's esophagus and esophageal adenocarcinoma

Thu Jul 14 00:00:00 EDT 2011

Vaccination with a plasmid DNA encoding HER-2/neu together with low doses of GM-CSF and IL-2 in patients with metastatic breast carcinoma: a pilot clinical trial.

Journal of translational medicine

Norell H,Poschke I,Charo J,Wei WZ,Erskine C,Piechocki MP,Knutson KL,Bergh J,Lidbrink E,Kiessling R

MA5-13672 was used in immunohistochemistry to study the therapeutic potential of HER-2/neu vaccination plus low doses of GM-CSF and IL-2 in metastatic breast carcinoma

Mon Jun 07 00:00:00 EDT 2010

Tiam1-deficiency impairs mammary tumor formation in MMTV-c-neu but not in MMTV-c-myc mice.

Journal of cancer research and clinical oncology

Strumane K,Rygiel T,van der Valk M,Collard JG

MA5-13672 was used in western blot to study the role of tiam1 in mammary tumor formation in MMTV-c-neu and MMTV-c-myc mice

Thu Jan 01 00:00:00 EST 2009

HER-2/neu (c-erbB-2) oncoprotein in hyperplastic endometrial polyps detected in two cats.

Journal of feline medicine and surgery

Misirlioglu D,Nak D,Ozyigit MO,Nak Y,Akkoc A

MA5-13672 was used in immunohistochemistry to study the HER-2/neu (c-erbB-2) oncoprotein in hyperplastic endometrial polyps detected in two cats

Thu Oct 01 00:00:00 EDT 2009

Ovarian adenocarcinomas in the laying hen and women share similar alterations in p53, ras, and HER-2/neu.

Cancer prevention research (Philadelphia, Pa.)

Hakim AA,Barry CP,Barnes HJ,Anderson KE,Petitte J,Whitaker R,Lancaster JM,Wenham RM,Carver DK,Turbov J,Berchuck A,Kopelovich L,Rodriguez GC

MA5-13672 was used in immunohistochemistry to investigate the similar changes of p53, ras, and HER-2/neu genes in ovatian adenocarcinomas between chicken and human

Sun Feb 01 00:00:00 EST 2009

Trastuzumab activates allogeneic or autologous antibody-dependent cellular cytotoxicity against malignant rhabdoid tumor cells and interleukin-2 augments the cytotoxicity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Katsumi Y,Kuwahara Y,Tamura S,Kikuchi K,Otabe O,Tsuchiya K,Iehara T,Kuroda H,Hosoi H,Sugimoto T

MA5-13672 was used in immunocytochemistry to investigate the mechanism for the malignant rhabdoid tumor treatment with trastuzumab

Fri Feb 15 00:00:00 EST 2008

Epitope mapping and structural analysis of an anti-ErbB2 antibody A21: Molecular basis for tumor inhibitory mechanism.

Proteins

Hu S,Zhu Z,Li L,Chang L,Li W,Cheng L,Teng M,Liu J

MA5-13672 was used in western blot to study the tumor inhibitory mechanism of an anti-ErbB2 antibody

Fri Feb 15 00:00:00 EST 2008

Loss of annexin A1 expression in breast cancer progression.

Applied immunohistochemistry & molecular morphology : AIMM

Cao Y,Li Y,Edelweiss M,Arun B,Rosen D,Resetkova E,Wu Y,Liu J,Sahin A,Albarracin CT

MA5-13672 was used in immunohistochemistry to study ANXA1 expression during the progression of breast carcinomas

Mon Dec 01 00:00:00 EST 2008

Breast cancer survival and tumor characteristics in premenopausal women carrying the CHEK2*1100delC germline mutation.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Schmidt MK,Tollenaar RA,de Kemp SR,Broeks A,Cornelisse CJ,Smit VT,Peterse JL,van Leeuwen FE,Van't Veer LJ

MA5-13672 was used in immunohistochemistry to study breast cancer survival and tumor characteristics in premenopausal women carrying the CHEK2*1100delC germline mutation

Mon Jan 01 00:00:00 EST 2007

Anti-EGFR and ErbB-2 antibodies attenuate cyclooxygenase-2 expression and cooperatively inhibit survival of human colon cancer cells.

Cancer letters

Half E,Sun Y,Sinicrope FA

MA5-13672 was used in western blot to examine the effect of anti-EGFR and ErbB-2 antibodies on the survival of human colon cancer cells

Thu Jun 28 00:00:00 EDT 2007

Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas.

Breast cancer research : BCR

Kreike B,van Kouwenhove M,Horlings H,Weigelt B,Peterse H,Bartelink H,van de Vijver MJ

MA5-13672 was used in immunohistochemistry to study triple negative breast carcinoma histopathology and gene expression patterns in order to define clinically significant sub-groups

Tue Mar 11 00:00:00 EDT 2008

Kinetics of serum HER-2/neu changes in patients with HER-2-positive primary breast cancer after initiation of primary chemotherapy.

Cancer

Mazouni C,Hall A,Broglio K,Fritsche H,Andre F,Esteva FJ,Hortobagyi GN,Buzdar AU,Pusztai L,Cristofanilli M

MA5-13672 was used in immunohistochemistry to study the value of serum HER-2 receptor extracellular domain measurements for predicting the response to chemotherapy in primary breast cancer patients

Thu Feb 01 00:00:00 EST 2007

ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells.

American journal of physiology. Lung cellular and molecular physiology

Zscheppang K,Liu W,Volpe MV,Nielsen HC,Dammann CE

MA5-13672 was used in immunocytochemistry and western blot to study the role of ErbB4 in regulating fetal surfactant phospholipid synthesis in primary fetal rat type II cells

Wed Aug 01 00:00:00 EDT 2007

Primary neuroendocrine carcinoma of the breast: a case report.

Tumori

Yaren A,Kelten C,Akbulut M,Teke Z,Duzcan E,Erdem E

MA5-13672 was used in immunohistochemistry to report on a case of primary neuroendocrine carcinoma of the breast

Tue Dec 11 00:00:00 EST 2007

Abundance and localization of skeletal muscle-related erbB2 may stimulate tumour growth during initial stages of oral oncogenesis.

Journal of musculoskeletal & neuronal interactions

Vairaktaris E,Moulavassili P,Loukeri S,Spyridonidou S,Yapijakis C,Vassiliou S,Nkenke E,Vylliotis A,Papakosta V,Lazaris A,Patsouris E

MA5-13672 was used in immunohistochemistry to study the role of skeletal muscle-related erbB2 in stimulating tumor growth in oral oncogenesis

Wed Oct 17 00:00:00 EDT 2007

EGF prevents the neuroendocrine differentiation of LNCaP cells induced by serum deprivation: the modulator role of PI3K/Akt.

Neoplasia (New York, N.Y.)

Martín-Orozco RM,Almaraz-Pro C,Rodríguez-Ubreva FJ,Cortés MA,Ropero S,Colomer R,López-Ruiz P,Colás B

MA5-13672 was used in western blot to investigate the impact of epidermal growth factor on neuroendocrine differentiation of LNCaP cells

Wed Aug 01 00:00:00 EDT 2007

Effect of c-neu/ ErbB2 expression levels on estrogen receptor alpha-dependent proliferation in mammary epithelial cells: implications for breast cancer biology.

Cancer research

Shyamala G,Chou YC,Cardiff RD,Vargis E

MA5-13672 was used in immunohistochemistry to study the effect of c-neu/ ErbB2 expression levels on estrogen receptor alpha-dependent proliferation in mammary epithelial cells

Wed Nov 01 00:00:00 EST 2006

ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.

Pediatric pulmonology

Zscheppang K,Korenbaum E,Bueter W,Ramadurai SM,Nielsen HC,Dammann CE

MA5-13672 was used in immunocytochemistry and immunoprecipitation to study erbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells

Fri Dec 01 00:00:00 EST 2006

MUC4 expression and its relation to ErbB2 expression, apoptosis, proliferation, differentiation, and tumor stage in non-small cell lung cancer (NSCLC).

Pathology, research and practice

Karg A,Dinç ZA,Başok O,Uçvet A

MA5-13672 was used in immunohistochemistry to study the expression of MUC4 in different stages of non-small cell lung cancer and its relationship with ErbB2 expression

Thu Sep 21 00:00:00 EDT 2006

Gene targeting of ErbB3 using a Cre-mediated unidirectional DNA inversion strategy.

Genesis (New York, N.Y. : 2000)

Qu S,Rinehart C,Wu HH,Wang SE,Carter B,Xin H,Kotlikoff M,Arteaga CL

MA5-13672 was used in western blot to study the efficacy of gene targeting of ErbB3 using an in vivo recombination unidirectional DNA inversion technology

Sun Oct 01 00:00:00 EDT 2006

[An analysis of genetic transmission in a father and son with osteosarcoma].

Acta orthopaedica et traumatologica turcica

Tokatli F,Alas RC,Altaner S,Pala F,Uygun K,Uzal C,Yalniz E

MA5-13672 was used in immunohistochemistry to report on a case of a father and son with familial osteosarcoma

Fri Jul 20 00:00:00 EDT 2007

Effect of Herceptin on the development and progression of skeletal metastases in a xenograft model of human breast cancer.

Oncogene

Khalili P,Arakelian A,Chen G,Singh G,Rabbani SA

MA5-13672 was used in immunohistochemistry and western blot to study the effect of Herceptin on the development and progression of skeletal metastases in a xenograft model of human breast cancer

Thu Oct 06 00:00:00 EDT 2005

Vitamin D receptor agonists induce prostatic acid phosphatase to reduce cell growth and HER-2 signaling in LNCaP-derived human prostate cancer cells.

The Journal of steroid biochemistry and molecular biology

Stewart LV,Lyles B,Lin MF,Weigel NL

MA5-13672 was used in western blot to study the effects of vitamin D receptor agonists on androgen-dependent LNCaP human prostate cancer cell growth

Sat Oct 01 00:00:00 EDT 2005

Investigation of three new mouse mammary tumor cell lines as models for transforming growth factor (TGF)-beta and Neu pathway signaling studies: identification of a novel model for TGF-beta-induced epithelial-to-mesenchymal transition.

Breast cancer research : BCR

Lenferink AE,Magoon J,Cantin C,O'Connor-McCourt MD

MA5-13672 was used in immunoprecipitation to study three novel mouse mammary tumor cell lines for use in TGF-beta and Neu pathway signaling studies

Tue Oct 05 00:00:00 EDT 2004

Vitamin D receptor status alters mammary gland morphology and tumorigenesis in MMTV-neu mice.

Carcinogenesis

Zinser GM,Welsh J

MA5-13672 was used in immunohistochemistry to study the effect of vitamin D receptor gene dosage on mammary gland morphology and tumorigenesis

Wed Dec 01 00:00:00 EST 2004

Automated quantification of apoptosis after neoadjuvant chemotherapy for breast cancer: early assessment predicts clinical response.

Clinical cancer research : an official journal of the American Association for Cancer Research

Davis DW,Buchholz TA,Hess KR,Sahin AA,Valero V,McConkey DJ

MA5-13672 was used in immunohistochemistry to study whether early assessment of apoptosis following breast cancer neoadjuvant chemotherapy predicts clinical response

Sat Mar 01 00:00:00 EST 2003

Establishment and characterization of cancer cell cultures and xenografts derived from primary or metastatic Mullerian cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Verschraegen CF,Hu W,Du Y,Mendoza J,Early J,Deavers M,Freedman RS,Bast RC,Kudelka AP,Kavanagh JJ,Giovanella BC

MA5-13672 was used in immunocytochemistry to characterize cell cultures and xenografts derived from patients with ovarian cancer

Sat Feb 01 00:00:00 EST 2003

Profiling receptor tyrosine kinase activation by using Ab microarrays.

Proceedings of the National Academy of Sciences of the United States of America

Nielsen UB,Cardone MH,Sinskey AJ,MacBeath G,Sorger PK

MA5-13672 was used in ELISA to study the receptor tyrosine kinase activation by through antibody microarrays

Tue Aug 05 00:00:00 EDT 2003

Breast carcinoma in pregnant women: assessment of clinicopathologic and immunohistochemical features.

Cancer

Middleton LP,Amin M,Gwyn K,Theriault R,Sahin A

MA5-13672 was used in immunohistochemistry to study the histopathology and immunohistochemistry of breast carcinoma in pregnant women

Mon Sep 01 00:00:00 EDT 2003

Mucin-rich variant of salivary duct carcinoma: a clinicopathologic and immunohistochemical study of four cases.

The American journal of surgical pathology

Simpson RH,Prasad AR,Lewis JE,Skálová A,David L

MA5-13672 was used in immunohistochemistry to study the clinicopathology and immunohistochemistry of four cases of mucin-rich variant of salivary duct carcinoma

Fri Aug 01 00:00:00 EDT 2003

A chimeric multi-human epidermal growth factor receptor-2 B cell epitope peptide vaccine mediates superior antitumor responses.

Journal of immunology (Baltimore, Md. : 1950)

Dakappagari NK,Pyles J,Parihar R,Carson WE,Young DC,Kaumaya PT

MA5-13672 was used in western blot to develop and evaluate a multi-epitope vaccine targeting HER-2

Tue Apr 15 00:00:00 EDT 2003

Her2/neu overexpression in differentiated thyroid carcinomas predicts metastatic disease.

Virchows Archiv : an international journal of pathology

Kremser R,Obrist P,Spizzo G,Erler H,Kendler D,Kemmler G,Mikuz G,Ensinger C

MA5-13672 was used in immunohistochemistry to study the prognostic value of Her2/neu overexpression in differentiated thyroid carcinomas

Tue Apr 01 00:00:00 EST 2003

Role of MHC II affinity and molecular mimicry in defining anti-HER-2/neu MAb-3 linear peptide epitope.

Peptides

Lucchese A,Stevanovic S,Sinha AA,Mittelman A,Kanduc D

MA5-13672 was used in western blot to study whether it is possible to predict which peptides will evoke a humoral immune response using the human HER-2/neu breast cancer-associated antigen as a model

Sat Feb 01 00:00:00 EST 2003

Cyclooxygenase-2 expression in human breast cancers and adjacent ductal carcinoma in situ.

Cancer research

Half E,Tang XM,Gwyn K,Sahin A,Wathen K,Sinicrope FA

MA5-13672 was used in immunohistochemistry to study the expression of COX-2 in human breast cancers and adjacent ductal carcinoma in situ

Fri Mar 15 00:00:00 EST 2002

Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome.

International journal of cancer

Mittelman A,Lucchese A,Sinha AA,Kanduc D

MA5-13672 was used in western blot to study the immune response to EC HER-2/NEU peptides not shared by the host's proteome

Wed Apr 10 00:00:00 EDT 2002

Classification of low-grade neuroendocrine tumors of midgut and unknown origin.

Human pathology

Van Eeden S,Quaedvlieg PF,Taal BG,Offerhaus GJ,Lamers CB,Van Velthuysen ML

MA5-13672 was used in immunohistochemistry to study whether refining the grading of low-grade neuroendocrine tumors of the midgut and unknown origin is of prognostic/predictive value

Fri Nov 01 00:00:00 EST 2002

Tyrphostin AG825 triggers p38 mitogen-activated protein kinase-dependent apoptosis in androgen-independent prostate cancer cells C4 and C4-2.

Cancer research

Murillo H,Schmidt LJ,Tindall DJ

MA5-13672 was used in western blot to study the role of p38 MAP kinase in the induction of apoptosis in androgen-independent prostate cancer cells by the EGFR inhibitor typhostin AG825

Mon Oct 15 00:00:00 EDT 2001

The tyrosine kinase inhibitor ZD1839 ("Iressa") inhibits HER2-driven signaling and suppresses the growth of HER2-overexpressing tumor cells.

Cancer research

Moasser MM,Basso A,Averbuch SD,Rosen N

MA5-13672 was used in western blot to study the inhibition of HER2-driven signaling and growth suppression of HER2-overexpressing tumor cells by Iressa

Mon Oct 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: avian erythroblastosis oncogene B 2; c-erb B2/neu protein; c-erbB-2; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; Neu oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

View more View less

Gene Aliases: c-erbB2; c-neu; CD340; Erbb-2; ERBB2; HER-2; HER-2/neu; HER2; Kiaa3023; mKIAA3023; MLN 19; MLN19; NEU; NGL; TKR1

View more View less

UniProt ID: (Human) P04626, (Mouse) P70424

View more View less

Entrez Gene ID: (Human) 2064, (Mouse) 13866

View more View less

Function(s)

RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity nucleotide binding glycoprotein binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding Hsp90 protein binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ErbB2 (HER-2) Monoclonal Antibody (3B5), Biotin

ErbB2 (HER-2) Antibody (MA5-13672) in WB

Product Details

MA5-13672

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!