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Product Details
MA5-31126
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
049
Immunogen
Recombinant Mouse ErbB4/HER4 Protein (Met1-Leu652)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2786776
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse ErbB4/HER4.
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: avian erythroblastosis oncogene B 4; OTTHUMP00000209432; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4
Gene Aliases: c-erbB-4; Erbb4; Her4; Mer4
UniProt ID: (Mouse) Q61527
Entrez Gene ID: (Mouse) 13869
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
epidermal growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein homodimerization activity
transcription regulatory region DNA binding
transmembrane signal receptor
neural crest cell migration
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
apoptotic process
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
nervous system development
heart development
lactation
positive regulation of cell proliferation
negative regulation of cell proliferation
embryonic pattern specification
negative regulation of muscle cell apoptotic process
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
central nervous system morphogenesis
olfactory bulb interneuron differentiation
regulation of cell migration
positive regulation of cell migration
positive regulation of synaptic transmission, GABAergic
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
surfactant homeostasis
mitochondrial fragmentation involved in apoptotic process
cell fate commitment
positive regulation of transcription, DNA-templated
positive regulation of glucose import
protein autophosphorylation
positive regulation of epithelial cell proliferation
positive regulation of cardiac muscle cell proliferation
synapse maturation
mammary gland epithelial cell differentiation
mammary gland alveolus development
cardiac muscle tissue regeneration
positive regulation of ERK1 and ERK2 cascade
positive regulation of STAT protein import into nucleus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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