Invitrogen

ErbB4 Recombinant Rabbit Monoclonal Antibody (8E5D0)

View all (49) ErbB4 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-35228

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8E5D0

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1200-1308 of human ErbB4/HER4 (Q15303)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849132

Product Specific Information

Immunogen sequence: DEYVNEPLYL NTFANTLGKA EYLKNNILSM PEKAKKAFDN PDYWNHSLPP RSTLQHPDYL QEYSTKYFYK QNGRIRPIVA ENPEYLSEFS LKPGTVLPPP PYRHRNTVV

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: avian erythroblastosis oncogene B 4; c-erbB-4; OTTHUMP00000209432; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-4; v-erb-a erythroblastic leukemia viral oncogene homolog 4

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Gene Aliases: c-erbB-4; Erbb4; Her4; Mer4; Tyro-2

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UniProt ID: (Mouse) Q61527, (Rat) Q62956

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Entrez Gene ID: (Mouse) 13869, (Rat) 59323

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Function(s)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity epidermal growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein homodimerization activity transcription regulatory region DNA binding transmembrane signal receptor
Process(es)
neural crest cell migration transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation apoptotic process signal transduction transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development nervous system development heart development lactation positive regulation of cell proliferation negative regulation of cell proliferation embryonic pattern specification negative regulation of muscle cell apoptotic process phosphorylation cell migration peptidyl-tyrosine phosphorylation central nervous system morphogenesis olfactory bulb interneuron differentiation regulation of cell migration positive regulation of cell migration positive regulation of synaptic transmission, GABAergic positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process surfactant homeostasis mitochondrial fragmentation involved in apoptotic process cell fate commitment positive regulation of transcription, DNA-templated positive regulation of glucose import protein autophosphorylation positive regulation of epithelial cell proliferation positive regulation of cardiac muscle cell proliferation synapse maturation mammary gland epithelial cell differentiation mammary gland alveolus development cardiac muscle tissue regeneration positive regulation of ERK1 and ERK2 cascade positive regulation of STAT protein import into nucleus telencephalon development signal transduction by protein phosphorylation mammary gland development response to progesterone odontogenesis response to drug response to hydrogen peroxide positive regulation of phosphatidylinositol 3-kinase activity
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