Invitrogen

ErbB4 (Her4) Monoclonal Antibody (HFR1), eBioscience™

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

14-9687-80

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Immunoprecipitation (IP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

HFR1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572955

Product Specific Information

Description: The monoclonal antibody HFR1 recognizes human and mouse epidermal growth factor receptor 4 (ErbB4 or HER4). ErbB4 belongs to the family of receptor tyrosine kinases (RTK) of which HER1 (EGRF), HER2 (ErbB2), and HER3 (ErbB3) are members. RTKs function to affect a variety of cell processes including proliferation, differentiation, migration, survival and apoptosis and are often disregulated in cancer. The ErbB4 protein contains extracellular ligand binding domains, a transmembrane region, and a cytoplasmic tail containing the tyrosine kinase domain and the binding site for the HFR1 antibody. Upon ligand binding, ErbB4 homo- or hetero-dimerizes with other family members, becomes phosphorylated, and can be cleaved allowing translocation to the nucleus. The HRF1 antibody recognizes membrane-bound, cytoplasmic and nuclear ErbB4. Cytosolic ErbB4 expression was found to correlate with a positive prognosis in a subset of breast cancer patients, while nuclear ErbB4 expression was inversely correlated with tumor grade and aggressiveness of breast tumors.

Applications Reported: This HFR1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HFR1 antibody has been tested by immunocytochemistry on fixed and permeablilized A431 cells and can be used at less than or equal to 10 µg/mL. The HFR1 antibody has also been tested by immunohistochemistry on human FFPE tissue with low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

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Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4

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UniProt ID: (Human) Q15303, (Mouse) Q61527

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Entrez Gene ID: (Human) 2066, (Mouse) 13869

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Function(s)
protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding protein binding ATP binding protein homodimerization activity transcription regulatory region DNA binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
MAPK cascade neural crest cell migration positive regulation of protein phosphorylation transcription, DNA-templated signal transduction transmembrane receptor protein tyrosine kinase signaling pathway nervous system development heart development lactation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation embryonic pattern specification regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling cell migration peptidyl-tyrosine phosphorylation central nervous system morphogenesis olfactory bulb interneuron differentiation regulation of cell migration ERBB2 signaling pathway positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of GTPase activity mitochondrial fragmentation involved in apoptotic process cell fate commitment positive regulation of transcription, DNA-templated protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of cardiac muscle cell proliferation mammary gland epithelial cell differentiation mammary gland alveolus development cardiac muscle tissue regeneration positive regulation of ERK1 and ERK2 cascade positive regulation of protein localization to cell surface regulation of cell motility positive regulation of STAT protein import into nucleus negative regulation of neuron migration regulation of transcription, DNA-templated protein phosphorylation apoptotic process multicellular organism development negative regulation of muscle cell apoptotic process phosphorylation positive regulation of cell migration positive regulation of synaptic transmission, GABAergic negative regulation of apoptotic process surfactant homeostasis positive regulation of glucose import positive regulation of epithelial cell proliferation synapse maturation
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