Invitrogen

Estrogen Receptor alpha Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

17 References

View all (172) Estrogen Receptor alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Estrogen Receptor alpha Antibody (PA1-308) in ICC/IF

Immunofluorescent analysis of Estrogen Receptor alpha (ER alpha, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 30 minutes at room temperature. Cells were stained with an ER alpha polyclonal antibody (Product... View More

Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)

Estrogen Receptor alpha Antibody in Western Blot (WB)

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Product Details

PA1-308

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

1-5 µg/mL

Immunocytochemistry (ICC/IF)

1:1,000-1:4,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325813

Product Specific Information

PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.

The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.

Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nitric Oxide Regulates Estrus Cycle Dependent Colonic Motility in Mice.

Frontiers in neuroscience

Balasuriya GK,Nugapitiya SS,Hill-Yardin EL,Bornstein JC

PA1-308 was used in Immunohistochemistry to confirm a cyclic and sexually dimorphic effect of NOS activity in female mouse colon, which could be due to genomic effects of estrogens via ERα.

Fri Oct 22 00:00:00 EDT 2021

6-Prenylnaringenin from Hops Disrupts ERα-Mediated Downregulation of CYP1A1 to Facilitate Estrogen Detoxification.

Chemical research in toxicology

Hitzman RT,Dunlap TL,Howell CE,Chen SN,Vollmer G,Pauli GF,Bolton JL,Dietz BM

PA1-308 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to investigate hop influence on estrogen carcinogenesis.

Mon Nov 16 00:00:00 EST 2020

A Sexually Dimorphic Area of the Dorsal Hypothalamus in Mice and Common Marmosets.

Endocrinology

Moe Y,Kyi-Tha-Thu C,Tanaka T,Ito H,Yahashi S,Matsuda KI,Kawata M,Katsuura G,Iwashige F,Sakata I,Akune A,Inui A,Sakai T,Ogawa S,Tsukahara S

PA1308 was used in immunohistochemistry to use mice to characterize the sexually dimorphic area in the dorsal hypothalamus

Thu Dec 01 00:00:00 EST 2016

Characterization of bladder sensory neurons in the context of myelination, receptors for pain modulators, and acute responses to bladder inflammation.

Frontiers in neuroscience

Forrest SL,Osborne PB,Keast JR

PA1-308 was used in immunohistochemistry to study bladder neurons involved in pain modulation and acute responses to inflammation

Wed Nov 13 00:00:00 EST 2013

Differential effects of steroids on the synthesis of polyunsaturated fatty acids by human neuroblastoma cells.

Neurochemistry international

Extier A,Perruchot MH,Baudry C,Guesnet P,Lavialle M,Alessandri JM

PA1-308 was used in western blot to investigate the effects of steroids on polyunsaturated fatty acid synthesis by human neuroblastoma cells

Tue Sep 01 00:00:00 EDT 2009

Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERalpha and ERbeta.

Maturitas

Cherdshewasart W,Panriansaen R,Picha P

PA1-308 was used in immunohistochemistry to investigate the protective effect of Pueraria mirifica phytoestrogens against rat mammary carcinoma tumors induced by DMBA

Sat Oct 20 00:00:00 EDT 2007

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo.

Endocrine

Shenouda NS,Sakla MS,Newton LG,Besch-Williford C,Greenberg NM,MacDonald RS,Lubahn DB

PA1-308 was used in western blot to investigate the effect of phytosterol Pygeum africanum on prostate cancer

Thu Feb 01 00:00:00 EST 2007

Genistein affects HER2 protein concentration, activation, and promoter regulation in BT-474 human breast cancer cells.

Endocrine

Sakla MS,Shenouda NS,Ansell PJ,Macdonald RS,Lubahn DB

PA1-308 was used in western blot to study the effect of genistein on HER2 expression and function in BT-474 human breast cancer cells

Wed Aug 01 00:00:00 EDT 2007

Androgen and estrogen receptor-mediated mechanisms of testosterone action in male rat pelvic autonomic ganglia.

Neuroscience

Purves-Tyson TD,Arshi MS,Handelsman DJ,Cheng Y,Keast JR

PA1-308 was used in immunohistochemistry to investigate the mechanisms of testosterone action in male rat pelvic autonomic ganglia

Fri Aug 10 00:00:00 EDT 2007

Apoptotic and anti-proliferative effects of 17beta-estradiol and 17beta-estradiol-like compounds in the Hep3B cell line.

Molecular and cellular biochemistry

Huang EJ,Wu CC,Huang HP,Liu JY,Lin CS,Chang YZ,Lin JA,Lin JG,Chen LM,Lee SD,Kuo WW,Huang CY

PA1-308 was used in western blot to study the apoptotic and anti-proliferative effects of estrogen and estrogen-like compounds on the human hepatoma Hep3B cell line

Sun Oct 01 00:00:00 EDT 2006

Rapid actions of estradiol on cyclic amp response-element binding protein phosphorylation in dorsal root ganglion neurons.

Neuroscience

Purves-Tyson TD,Keast JR

PA1-308 was used in immunocytochemistry to investigate the effect of estrogens on the CREB-mediated transcriptional regulation in sensory neurons

Mon Mar 07 00:00:00 EST 2005

Phytoestrogens in common herbs regulate prostate cancer cell growth in vitro.

Nutrition and cancer

Shenouda NS,Zhou C,Browning JD,Ansell PJ,Sakla MS,Lubahn DB,Macdonald RS

PA1-308 was used in western blot to investigate the effect of phytoestrogens on prostate tumor growth

Fri Apr 08 00:00:00 EDT 2005

Multiple forms of estrogen receptor-alpha in cerebral blood vessels: regulation by estrogen.

American journal of physiology. Endocrinology and metabolism

Stirone C,Duckles SP,Krause DN

PA1-308 was used in western blot to investigate the expression, localization, and regulation of ER-alpha in female rat cerebral vasculature

Wed Jan 01 00:00:00 EST 2003

Sex difference in septal neurons projecting axons to midbrain central gray in rats: a combined double retrograde tracing and ER-immunohistochemical study.

Endocrinology

Tsukahara S,Yamanouchi K

PA1-308 was used in immunohistochemistry to investigate the sexual dimorphism in septal neurons projecting axons to midbrain central gray in rats.

Tue Jan 01 00:00:00 EST 2002

17-Beta-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis.

The Journal of steroid biochemistry and molecular biology

Kramer PR,Wray S

PA1-308 was used in western blot to investigate the regulation of 17-beta-estradiol on the expression of genes functioning in macrophage activation and cholesterol metabolism

Mon Jul 01 00:00:00 EDT 2002

Liganded androgen receptor interaction with beta-catenin: nuclear co-localization and modulation of transcriptional activity in neuronal cells.

The Journal of biological chemistry

Pawlowski JE,Ertel JR,Allen MP,Xu M,Butler C,Wilson EM,Wierman ME

PA1-308 was used in immunocytochemistry to investigate the association between beta-catenin and androgen receptor.

Fri Jun 07 00:00:00 EDT 2002

Cancer-associated myofibroblasts possess various factors to promote endometrial tumor progression.

Clinical cancer research : an official journal of the American Association for Cancer Research

Orimo A,Tomioka Y,Shimizu Y,Sato M,Oigawa S,Kamata K,Nogi Y,Inoue S,Takahashi M,Hata T,Muramatsu M

PA1-308 was used in immunohistochemistry to demonstrate the role of cancer-associated myofibroblasts in endometrial tumor progression.

Mon Oct 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: DKFZp686N23123; ER; Er alpha; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

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Gene Aliases: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR

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UniProt ID: (Human) P03372, (Rat) P06211

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Entrez Gene ID: (Human) 2099, (Rat) 24890

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding

Process(es)

Performance Guarantee

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Estrogen Receptor alpha Polyclonal Antibody

Estrogen Receptor alpha Antibody (PA1-308) in ICC/IF

Product Details

PA1-308

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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