Invitrogen

Estrogen Receptor alpha Monoclonal Antibody (33)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

14 Published Figures
40 References
View all (172) Estrogen Receptor alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-310

Applications
Tested Dilution
Publications

Western Blot (WB)

4 µg/mL
View 16 publications 16 publications

Immunohistochemistry (IHC)

1:20 - 1:100
View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

10 µg/mL
View 7 publications 7 publications

Flow Cytometry (Flow)

-
View 2 publications 2 publications

Immunoprecipitation (IP)

-
View 2 publications 2 publications

Gel Shift (GS)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Avian, Bovine, Fish, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

33

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

Dulbecco's PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325422

Product Specific Information

MA1-310 detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. MA1-310 detects ER alpha and does not cross react with ER beta.

MA1-310 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, gel shift, flow cytometry, and immunocytochemical experiments. By Western blot, this antibody detects an ~66 kDa protein representing ER alpha from MCF-7 cell extract.

MA1-310 can be used with blocking peptide PEP-013.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686N23123; ER; Er alpha; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; hER-alpha36; nuclear receptor; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

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Gene Aliases: ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1; RNESTROR

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UniProt ID: (Human) P03372, (Bovine) P49884, (Mouse) P19785, (Rat) P06211

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Entrez Gene ID: (Human) 2099, (Bovine) 407238, (Mouse) 13982, (Rat) 24890

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding TFIIB-class transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding TBP-class protein binding protein kinase binding estrogen receptor binding sequence-specific DNA binding DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth osteoblast development chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process negative regulation of gene expression positive regulation of phospholipase C activity intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity regulation of neuron apoptotic process response to estrogen positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import decidualization positive regulation of fibroblast proliferation negative regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell development Sertoli cell proliferation uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation positive regulation of ERK1 and ERK2 cascade protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus negative regulation of triglyceride metabolic process negative regulation of neuron death negative regulation of production of miRNAs involved in gene silencing by miRNA baculum development epithelial cell development regulation of transcription from RNA polymerase II promoter male gonad development intracellular receptor signaling pathway regulation of apoptotic process steroid hormone mediated signaling pathway positive regulation of nitric oxide biosynthetic process positive regulation of retinoic acid receptor signaling pathway positive regulation of nitric-oxide synthase activity regulation of toll-like receptor signaling pathway positive regulation of RNA polymerase II transcriptional preinitiation complex assembly stem cell differentiation regulation of inflammatory response
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