Invitrogen

Estrogen Receptor alpha Monoclonal Antibody (6F11)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures
37 References
View all (172) Estrogen Receptor alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-27107

Applications
Tested Dilution
Publications

Western Blot (WB)

1:50
View 3 publications 3 publications

Immunohistochemistry (IHC)

-
View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:80
View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:60
-

Immunocytochemistry (ICC/IF)

1:100
View 1 publication 1 publication

ChIP assay (ChIP)

5 µL/10^6 cells
-

Gel Shift (GS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cat, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F11

Immunogen

Recombinant estrogen receptor protein (alpha form)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_780508

Product Specific Information

Paraffin sections require an antigen retrieval pretreatment with sodium citrate buffer (pH 6.0) prior to staining. Use Samboni’s fixative for optimal staining results. Positive control of Breast carcinoma suggested.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686N23123; ER; Er alpha; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

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Gene Aliases: ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1; RNESTROR

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UniProt ID: (Human) P03372, (Mouse) P19785, (Rat) P06211

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Entrez Gene ID: (Human) 2099, (Mouse) 13982, (Rat) 24890

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth osteoblast development chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process negative regulation of gene expression positive regulation of phospholipase C activity intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity regulation of neuron apoptotic process response to estrogen positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import decidualization positive regulation of fibroblast proliferation negative regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell development Sertoli cell proliferation uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation positive regulation of ERK1 and ERK2 cascade protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus negative regulation of triglyceride metabolic process negative regulation of neuron death negative regulation of production of miRNAs involved in gene silencing by miRNA baculum development epithelial cell development regulation of transcription from RNA polymerase II promoter male gonad development intracellular receptor signaling pathway regulation of apoptotic process steroid hormone mediated signaling pathway positive regulation of nitric oxide biosynthetic process positive regulation of retinoic acid receptor signaling pathway positive regulation of nitric-oxide synthase activity
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