Invitrogen

Estrogen Receptor alpha Monoclonal Antibody (6F11)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

39 References

View all (172) Estrogen Receptor alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Estrogen Receptor alpha Antibody (MA1-80216) in ICC/IF

Immunofluorescence analysis of Estrogen Receptor alpha was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Estrogen Receptor alpha Monoclonal Antibody (6F11) (Product # MA1-80216) at 1:100 in 0.1% BSA, incu... View More

Estrogen Receptor alpha Antibody in Western Blot (WB)

Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA1-80216

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:80

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:60

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cat, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F11

Immunogen

Recombinant estrogen receptor protein (alpha form)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

< 0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_930763

Product Specific Information

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is breast carcinoma.

Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

JMJD6 Shapes a Pro-tumor Microenvironment via ANXA1-Dependent Macrophage Polarization in Breast Cancer.

Molecular cancer research : MCR

Cioni B,Ratti S,Piva A,Tripodi I,Milani M,Menichetti F,Langella T,Botti L,De Cecco L,Chiodoni C,Lecis D,Colombo MP

Published figure using Estrogen Receptor alpha monoclonal antibody (Product # MA1-80216) in Immunocytochemistry

Thu Jun 01 00:00:00 EDT 2023

Comparative Efficacy of AZD9496 and Fulvestrant on the Growth of Pituitary Adenoma via Blocking JAK2/STAT5B Pathway.

Journal of Cancer

Liu Q,Shen Y,Xiong Y,Bai J,Zhang Y,Li C

Published figure using Estrogen Receptor alpha monoclonal antibody (Product # MA1-80216) in Immunohistochemistry

Wed Jan 11 00:00:00 EST 2023

Enhanced anti-cancer effects of oestrogen and progesterone co-therapy against colorectal cancer in males.

Frontiers in endocrinology

Mahbub AA,Aslam A,Elzubier ME,El-Boshy M,Abdelghany AH,Ahmad J,Idris S,Almaimani R,Alsaegh A,El-Readi MZ,Baghdadi MA,Refaat B

Published figure using Estrogen Receptor alpha monoclonal antibody (Product # MA1-80216) in Immunohistochemistry

Fri Oct 21 00:00:00 EDT 2022

The bone microenvironment increases phenotypic plasticity of ER⁺ breast cancer cells.

Developmental cell

Bado IL,Zhang W,Hu J,Xu Z,Wang H,Sarkar P,Li L,Wan YW,Liu J,Wu W,Lo HC,Kim IS,Singh S,Janghorban M,Muscarella AM,Goldstein A,Singh P,Jeong HH,Liu C,Schiff R,Huang S,Ellis MJ,Gaber MW,Gugala Z,Liu Z,Zhang XH

MA1-80216 was used in Immunohistochemistry to exemplify how epigenomic adaptation to BME promotes phenotypic plasticity of metastatic seeds, fosters intra-metastatic heterogeneity, and alters therapeutic responses.

Mon Apr 19 00:00:00 EDT 2021

Estrogen-Dependent Functional Spine Dynamics in Neocortical Pyramidal Neurons of the Mouse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ye Z,Cudmore RH,Linden DJ

MA1-80216 was used in Immunohistochemistry to demonstrate that estrogen replacement reverses some of the effects of surgical removal of the ovaries on the structure and function of brain cells in mice..

Wed Jun 19 00:00:00 EDT 2019

St Gallen molecular subtypes in feline mammary carcinoma and paired metastases-disease progression and clinical implications from a 3-year follow-up study.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Soares M,Correia J,Peleteiro MC,Ferreira F

MA180216 was used in immunohistochemistry to describe the clinical, pathological, and immunophenotypic features collected from 61 queens with feline mammary carcinoma

Tue Mar 01 00:00:00 EST 2016

Serum HER2 levels are increased in cats with mammary carcinomas and predict tissue HER2 status.

Oncotarget

Soares M,Ribeiro R,Najmudin S,Gameiro A,Rodrigues R,Cardoso F,Ferreira F

MA180216 was used in immunohistochemistry - paraffin section to develop an assay to measure serum HER2 in order to diagnose cats with mammary carcinomas

Tue Apr 05 00:00:00 EDT 2016

Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo.

BMC cancer

Rowson-Hodel AR,Manjarin R,Trott JF,Cardiff RD,Borowsky AD,Hovey RC

MA1-80216 was used in immunohistochemistry to characterize porcine mammary epithelial cells in vitro and tumor formation in vivo and neoplastic transformation

Fri Jul 31 00:00:00 EDT 2015

Unraveling the regulatory connections between two controllers of breast cancer cell fate.

Nucleic acids research

Lee J,Tiwari A,Shum V,Mills GB,Mancini MA,Igoshin OA,Balázsi G

MA1-80216 was used in western blot to study the roles of ER-alpha and GATA3 in regulating the fate of breast cancer cells

Sun Jun 01 00:00:00 EDT 2014

Progesterone and estrogen receptors in conjunctival melanoma and nevi.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Bredow L,Stützel L,Böhringer D,Gundlach E,Reinhard T,Auw-Haedrich C

MA1-80216 was used in immunohistochemistry to study the clinical significance of the expression of receptors for progesterone and estrogen in conjunctival melanoma and nevi

Sat Feb 01 00:00:00 EST 2014

Tissue biomarkers in prognostication of serous ovarian cancer following neoadjuvant chemotherapy.

BioMed research international

Khandakar B,Mathur SR,Kumar L,Kumar S,Datta Gupta S,Iyer VK,Kalaivani M

MA1-80216 was used in immunohistochemistry to attempt to identify prognostic biomarkers in serous ovarian cancer patients treated with neoadjuvant chemotherapy

Mon Jan 19 00:00:00 EST 2015

Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors.

Oncology reports

Corrêa NC,Kuasne H,Faria JA,Seixas CC,Santos IG,Abreu FB,Nonogaki S,Rocha RM,Aparecida Borges Silva G,Gobbi H,Rogatto SR,Goes AM,Gomes DA

MA1-80216 was used in immunohistochemistry - paraffin section to characterize Brazilian breast cancer cell lines

Mon Apr 01 00:00:00 EDT 2013

Breast cancer tissue slices as a model for evaluation of response to rapamycin.

Cell and tissue research

Grosso SH,Katayama ML,Roela RA,Nonogaki S,Soares FA,Brentani H,Lima L,Folgueira MA,Waitzberg AF,Pasini FS,Góes JC,Brentani MM

MA1-80216 was used in immunohistochemistry to study the response to rapamycin therapy using organotypic breast cancer tissue slices and the potential utility in personalized therapy

Sat Jun 01 00:00:00 EDT 2013

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Clinical biochemistry

Kruer TL,Cummins TD,Powell DW,Wittliff JL

MA1-80216 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer

Fri Nov 01 00:00:00 EDT 2013

Epigenetic reactivation of estrogen receptor-α (ERα) by genistein enhances hormonal therapy sensitivity in ERα-negative breast cancer.

Molecular cancer

Li Y,Meeran SM,Patel SN,Chen H,Hardy TM,Tollefsbol TO

MA1-80216 was used in immunohistochemistry and western blot to study the ability of genistein to epigenetically reactivate ER-alpha expression in ER-alpha-negative breast cancer and enhance tamoxifen sensitivity

Mon Feb 04 00:00:00 EST 2013

Breast epithelial cell proliferation is markedly increased with short-term high levels of endogenous estrogen secondary to controlled ovarian hyperstimulation.

Breast cancer research and treatment

Chung K,Hovanessian-Larsen LJ,Hawes D,Taylor D,Downey S,Spicer DV,Stanczyk FZ,Patel S,Anderson AR,Pike MC,Wu AH,Pearce CL

MA1-80216 was used in immunohistochemistry to study the effect of short-term high endogenous estrogen exposure on human breast epithelial cell proliferation

Sun Apr 01 00:00:00 EDT 2012

Prediction of BRCA2-association in hereditary breast carcinomas using array-CGH.

Breast cancer research and treatment

Joosse SA,Brandwijk KI,Devilee P,Wesseling J,Hogervorst FB,Verhoef S,Nederlof PM

MA1-80216 was used in immunohistochemistry to develop a method to predict BRCA2-association in hereditary breast carcinomas

Sun Apr 01 00:00:00 EDT 2012

Lowering oral contraceptive norethindrone dose increases estrogen and progesterone receptor levels with no reduction in proliferation of breast epithelium: a randomized trial.

Contraception

Hovanessian-Larsen L,Taylor D,Hawes D,Spicer DV,Press MF,Wu AH,Pike MC,Pearce CL

MA1-80216 was used in immunohistochemistry to study the expression of estrogen and progesterone receptors and the degree of breast epithelial proliferation in women taking oral contraceptives with different levels of norethindrone

Sat Sep 01 00:00:00 EDT 2012

Histopathology

Logullo AF,Stiepcich MM,Osório CA,Nonogaki S,Pasini FS,Rocha RM,Soares FA,Brentani MM

MA1-80216 was used in immunohistochemistry to study the prognostic value of Fos-related antigen 1 in ductal breast carcinoma

Tue Mar 01 00:00:00 EST 2011

Genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors.

Genes, chromosomes & cancer

Joosse SA,Brandwijk KI,Mulder L,Wesseling J,Hannemann J,Nederlof PM

MA1-80216 was used in immunohistochemistry to study the genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors

Tue Feb 01 00:00:00 EST 2011

CDB-4124, a progesterone receptor modulator, inhibits mammary carcinogenesis by suppressing cell proliferation and inducing apoptosis.

Cancer prevention research (Philadelphia, Pa.)

Wiehle R,Lantvit D,Yamada T,Christov K

MA1-80216 was used in immunohistochemistry to study the mechanism by which a progesterone receptor modulator inhibits mammary carcinogenesis

Tue Mar 01 00:00:00 EST 2011

Breast cancer subtypes and response to systemic treatment after whole-brain radiotherapy in patients with brain metastases.

Cancer

Niwińska A,Murawska M,Pogoda K

MA1-80216 was used in immunohistochemistry to study the effect of breast cancer subtype on the response to systemic treatment following whole-brain radiotherapy in patients with brain metastases

Wed Sep 15 00:00:00 EDT 2010

Microarray-based determination of estrogen receptor, progesterone receptor, and HER2 receptor status in breast cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Roepman P,Horlings HM,Krijgsman O,Kok M,Bueno-de-Mesquita JM,Bender R,Linn SC,Glas AM,van de Vijver MJ

MA1-80216 was used in immunohistochemistry to investigate the expression level of estrogen receptor, progesterone receptor, and HER2 receptor in breast cancer

Sun Nov 15 00:00:00 EST 2009

Progesterone and estrogen receptors in pregnant and premenopausal non-pregnant normal human breast.

Breast cancer research and treatment

Taylor D,Pearce CL,Hovanessian-Larsen L,Downey S,Spicer DV,Bartow S,Pike MC,Wu AH,Hawes D

MA1-80216 was used in immunohistochemistry to study progesterone and estrogen receptor expression in normal breast of pregnant and premenopausal non-pregnant women

Sun Nov 01 00:00:00 EDT 2009

Gene expression profiling to identify the histogenetic origin of metastatic adenocarcinomas of unknown primary.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Horlings HM,van Laar RK,Kerst JM,Helgason HH,Wesseling J,van der Hoeven JJ,Warmoes MO,Floore A,Witteveen A,Lahti-Domenici J,Glas AM,Van't Veer LJ,de Jong D

MA1-80216 was used in immunohistochemistry to study the clinical value of gene expression profiling of metastatic adenocarcinomas of unknown primary

Sat Sep 20 00:00:00 EDT 2008

Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.

American journal of clinical pathology

Shi SR,Liu C,Pootrakul L,Tang L,Young A,Chen R,Cote RJ,Taylor CR

MA1-80216 was used in immunohistochemistry to evaluate different fixation and antigen retrieval methods for immmunohistochemical studies on frozen tissues

Sat Mar 01 00:00:00 EST 2008

Different prognostic roles of mutations in the helical and kinase domains of the PIK3CA gene in breast carcinomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Barbareschi M,Buttitta F,Felicioni L,Cotrupi S,Barassi F,Del Grammastro M,Ferro A,Dalla Palma P,Galligioni E,Marchetti A

MA1-80216 was used in immunohistochemistry to study the prognostic value of different PIK3CA gene mutations in breast cancer

Mon Oct 15 00:00:00 EDT 2007

Hormone receptor expressions and proliferation markers in postmenopausal endometrial polyps.

Gynecologic and obstetric investigation

Inceboz US,Nese N,Uyar Y,Ozcakir HT,Kurtul O,Baytur YB,Kandiloglu AR,Caglar H,Fraser IS

MA1-80216 was used in immunohistochemistry to study the expression of hormone receptors and proliferative markers in postmenopausal endometrial polyps

Tue Jan 30 00:00:00 EST 2007

Estrogen induced functional hypertrophy and increased force generation of the female rabbit bladder.

Neurourology and urodynamics

Lin AD,Levin R,Kogan B,Whitbeck C,Chichester P,Sokol R,Mannikarottu A

MA1-80216 was used in immunohistochemistry to examine the effect of estrogen in female rabbit bladder

Tue Oct 10 00:00:00 EDT 2006

The expression of Ki-67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study.

Gynecologic oncology

Akhan SE,Yavuz E,Tecer A,Iyibozkurt CA,Topuz S,Tuzlali S,Bengisu E,Berkman S

MA1-80216 was used in immunohistochemistry to study the role of Ki-67, p53, estrogen and progesterone receptors in uterine leiomyosarcomas

Sat Oct 01 00:00:00 EDT 2005

Epithelioid trophoblastic tumor of the broad ligament: a case report and review of the literature.

The American journal of surgical pathology

Kuo KT,Chen MJ,Lin MC

MA1-80216 was used in immunohistochemistry - paraffin section to present the case of a patient with an epithelioid trophoblastic tumor

Mon Mar 01 00:00:00 EST 2004

Abnormal morphology of the penis in male rats exposed neonatally to diethylstilbestrol is associated with altered profile of estrogen receptor-alpha protein, but not of androgen receptor protein: a developmental and immunocytochemical study.

Biology of reproduction

Goyal HO,Braden TD,Williams CS,Dalvi P,Mansour MM,Mansour M,Williams JW,Bartol FF,Wiley AA,Birch L,Prins GS

MA1-80216 was used in immunohistochemistry - paraffin section to determine the changes in morphology and expression of androgen receptor and estrogen receptor alpha in the body of the rat penis exposed neonatally to diethylstilbestrol

Sat May 01 00:00:00 EDT 2004

Expression of CYP3A4 in human breast tumour and non-tumour tissues.

Cancer letters

Kapucuoglu N,Coban T,Raunio H,Pelkonen O,Edwards RJ,Boobis AR,Iscan M

MA1-80216 was used in immunocytochemistry to study the expression of CYP3A4 protein in breast tumour and non-tumour tissues

Mon Dec 08 00:00:00 EST 2003

Simultaneous carcinoma of the endometrium and ovary vs endometrial carcinoma with ovarian metastases: a clinical and immunohistochemical determination.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Halperin R,Zehavi S,Hadas E,Habler L,Bukovsky I,Schneider D

MA1-80216 was used in immunohistochemistry - paraffin section to compare tumors involving endometrium and ovary and metastatic endometrial tumors

Tue Jun 03 00:00:00 EDT 2003

Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.

Molecular and cellular endocrinology

Zinser GM,McEleney K,Welsh J

MA1-80216 was used in western blot to characterize mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice

Fri Feb 28 00:00:00 EST 2003

Breast hamartomas: clinicopathological and immunohistochemical studies of 24 cases.

Histopathology

Herbert M,Sandbank J,Liokumovich P,Yanai O,Pappo I,Karni T,Segal M

MA1-80216 was used in immunohistochemistry - paraffin section to determine the clinical, pathological, and immunohistochemical features of breast hamartomas

Mon Jul 01 00:00:00 EDT 2002

Biophenotypes and survival of BRCA1 and TP53 deleted breast cancer in young women.

Breast cancer research and treatment

Querzoli P,Albonico G,di Iasio MG,Ferretti S,Rinaldi R,Cariello A,Pedriali M,Matteuzzi M,Maestri I,Nenci I

MA1-80216 was used in immunohistochemistry to study the effects of loss of heterozygosity of BRCA1 and TP53 on breast cancer survival in young women

Thu Mar 01 00:00:00 EST 2001

Immunohistochemical comparison of primary peritoneal and primary ovarian serous papillary carcinoma.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Halperin R,Zehavi S,Hadas E,Habler L,Bukovsky I,Schneider D

MA1-80216 was used in immunohistochemistry - paraffin section to compare matched primary peritoneal serous papillary carcinoma and ovarian serous papillary cancer samples

Mon Oct 01 00:00:00 EDT 2001

Apolipoprotein E polymorphism and breast carcinoma: correlation with cell proliferation indices and clinical outcome.

Breast cancer research and treatment

Zunarelli E,Nicoll JA,Migaldi M,Trentini GP

MA1-80216 was used in immunohistochemistry to study the prognostic significance of apolipoprotein E polymorphism in breast carcinoma

Sun Oct 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: DKFZp686N23123; ER; Er alpha; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

View more View less

Gene Aliases: ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1; RNESTROR

View more View less

UniProt ID: (Human) P03372, (Mouse) P19785, (Rat) P06211

View more View less

Entrez Gene ID: (Human) 2099, (Mouse) 13982, (Rat) 24890

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Estrogen Receptor alpha Monoclonal Antibody (6F11)

Estrogen Receptor alpha Antibody (MA1-80216) in ICC/IF

Product Details

MA1-80216

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!