Invitrogen

Estrogen Receptor beta Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

52 References

View all (57) Estrogen Receptor beta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Estrogen Receptor beta Antibody in Immunocytochemistry (ICC/IF)

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Estrogen Receptor beta Antibody (PA1-310B) in ICC/IF

Immunofluorescent analysis using ER-beta polyclonal antibody (Product # PA1-310B) shows nuclear localization of ER-beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ER-beta (Pro... View More

Estrogen Receptor beta Antibody in Western Blot (WB)

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Product Details

PA1-310B

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 5 publications 5 publications

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Pig, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(467) S S T E D S K N K E S S Q N L Q S Q(485) of rat ER beta.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325815

Product Specific Information

PA1-310B has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from both rat brain homogenate as well as various breast cancer cell lines. Immunocytochemical staining of ER beta in cells overexpressing the human protein results in nuclear staining.

The PA1-310B immunogen is a synthetic peptide corresponding to residues C(467) S S T E D S K N K E S S Q N L Q S Q(485) of rat ER beta. This immunizing peptide (PEP-007) is available for use in neutralization and control experiments.

Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sexual dimorphism in reactive oxygen species production and a role for integrin α1β1 in estrogen receptor α and β expression in articular cartilage.

Journal of orthopaedic surgery and research

Black AL,Haskins J,Pozzi A,Clark AL

PA1-310B was used in Immunohistochemistry-immunofluorescence to highlight sexual dimorphism in the EGFR/integrin α1β1 signaling axis and underline the need for further investigation into the role of ERs in this biological paradigm.

Mon Mar 06 00:00:00 EST 2023

Estrogen receptor beta in the central amygdala regulates the deleterious behavioral and neuronal consequences of repeated social stress in female rats.

Neurobiology of stress

Smiley CE,Pate BS,Bouknight SJ,Francis MJ,Nowicki AV,Harrington EN,Wood SK

PA1-310B was used in Western Blotting to indicate that ERβ signaling in the CeA, likely through its effects on CRF, contributes to the development of negative valence behaviors that result from exposure to repeated social stress in female rats.

Wed Mar 01 00:00:00 EST 2023

Obesity Associated with Prediabetes Increases the Risk of Breast Cancer Development and Progression-A Study on an Obese Rat Model with Impaired Glucose Tolerance.

International journal of molecular sciences

Kallamadi PR,Esari D,Addi UR,Kesavan R,Putcha UK,Nagini S,Reddy GB

PA1-310B was used in Immunohistochemistry to indicate that tumor onset and development were faster in spontaneous obese rat models with impaired glucose tolerance than in their lean counterparts.

Fri Jul 14 00:00:00 EDT 2023

Complex Extract of Polygonatum sibiricum and Nelumbinis semen Improves Menopause Symptoms via Regulation of Estrogen Receptor Beta in an Ovariectomized Rat Model.

Nutrients

Park D,Yoon JE,Choi B,Lee YJ,Ha IH

PA1-310B was used in Immunohistochemistry to identify the effect of a complex extract of Polygonatum sibiricum (PS) and Nelumbinis semen (NS) on improving menopause without side effects.

Wed May 24 00:00:00 EDT 2023

Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma.

Molecular oncology

Shin HY,Yang W,Chay DB,Lee EJ,Chung JY,Kim HS,Kim JH

PA1-310B was used in Western Blot to suggest the potential of TSPAN1 as a screening candidate for high-risk endometriosis.

Thu Apr 01 00:00:00 EDT 2021

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome.

Frontiers in psychiatry

Zhou XD,Yang XJ,Zheng Y,Qin ZS,Sha W,Chen G,Zhang ZJ

PA1-310B was used in Western Blot to suggest that JYP has comparable efficacy in ameliorating mood disorder-like behavior and cognitive impairment induced by a combination of estrogen deprivation and chronic stress in association with certain differential uterus-brain mechanisms compared to estrogen therapy.

Fri Dec 18 00:00:00 EST 2020

Silymarin, boswellic acid and curcumin enriched dietetic formulation reduces the growth of inherited intestinal polyps in an animal model.

World journal of gastroenterology

Girardi B,Pricci M,Giorgio F,Piazzolla M,Iannone A,Losurdo G,Principi M,Barone M,Ierardi E,Di Leo A

PA1-310B was used in Western Blotting to assess whether an enriched nutritional formulation (silymarin, boswellic acid and curcumin) with proven “in vitro” and “in vivo” anti-carcinogenetic properties may prevent inherited intestinal cancer in animal model.

Tue Apr 14 00:00:00 EDT 2020

Determining the endocrine disruption potential of industrial chemicals using an integrative approach: Public databases, in vitro exposure, and modeling receptor interactions.

Environment international

Alofe O,Kisanga E,Inayat-Hussain SH,Fukumura M,Garcia-Milian R,Perera L,Vasiliou V,Whirledge S

PA1-310B was used in Western Blotting to evaluate the endocrine disrupting potential of a broad range of chemicals used in the petrochemical sector.

Tue Oct 01 00:00:00 EDT 2019

Estrogen-Dependent Functional Spine Dynamics in Neocortical Pyramidal Neurons of the Mouse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ye Z,Cudmore RH,Linden DJ

PA1-310B was used in Immunohistochemistry to demonstrate that estrogen replacement reverses some of the effects of surgical removal of the ovaries on the structure and function of brain cells in mice..

Wed Jun 19 00:00:00 EDT 2019

Sexually dimorphic leanness and hypermobility in p16^Ink4a/CDKN2A-deficient mice coincides with phenotypic changes in the cerebellum.

Scientific reports

Kim KH,Cho Y,Lee J,Jeong H,Lee Y,Kim SI,Kim CH,Lee HW,Nam KT

PA1-310B was used in Immunohistochemistry-immunofluorescence to suggest that loss of p16INK4a induces sexually dimorphic leanness in female mice, which appears to be due to protection against cerebellar senescence by promoting neuronal proliferation and homeostasis via ERβ.

Thu Aug 01 00:00:00 EDT 2019

Young Coconut Juice Reduces Some Histopathological Changes Associated with Alzheimer's Disease through the Modulation of Estrogen Receptors in Orchidectomized Rat Brains.

Journal of aging research

Balit T,Abdel-Wahhab MA,Radenahmad N

PA1-310B was used in Immunohistochemistry to evaluate the protective role of young coconut juice (YCJ) against the pathological changes in Alzheimer's disease (AD) in orchidectomized (orx) rats.

Thu Oct 01 00:00:00 EDT 2020

27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines.

Biochimie

Warns J,Marwarha G,Freking N,Ghribi O

PA1-310B was used in Western Blotting to show for the first time that the cholesterol metabolite 27-OHC reduces cell proliferation in colorectal cancer cells.

Mon Oct 01 00:00:00 EDT 2018

Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria.

Aging

Evola CM,Hudson TL,Huang L,Corbett AM,Mayes DA

PA1-310B was used in Western Blotting to accentuate the complexity of aging and provide insight into the gender-specific cellular processes that occur throughout this process.

Fri Aug 31 00:00:00 EDT 2018

Developmental Toxicant Exposure Is Associated with Transgenerational Adenomyosis in a Murine Model.

Biology of reproduction

Bruner-Tran KL,Duleba AJ,Taylor HS,Osteen KG

PA1310B was used in immunohistochemistry - paraffin section to determine the incidence of adenomyosis and the expression of estrogen receptors in uterus of mice exposed to TCDD.

Sat Oct 01 00:00:00 EDT 2016

Placental STAT3 signaling is activated in women with polycystic ovary syndrome.

Human reproduction (Oxford, England)

Maliqueo M,Sundström Poromaa I,Vanky E,Fornes R,Benrick A,Åkerud H,Stridsklev S,Labrie F,Jansson T,Stener-Victorin E

PA1-310B was used in western blot to study STAT3 and mTOR signaling in patients with polycystic ovary syndrome

Sun Mar 01 00:00:00 EST 2015

Different expression of membrane-bound and cytosolic hippocampal steroid receptor complexes during spatial training in young male rats.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Sase S,Meyer K,Lubec G,Korz V

PA1-310B was used in western blot to examine the expression of membrane and cytosolic hippocampal receptors during spatial learning

Sat Nov 01 00:00:00 EDT 2014

Ductuli efferentes of the male Golden Syrian hamster reproductive tract.

Andrology

Ford J,Carnes K,Hess RA

PA1-310B was used in immunohistochemistry to study the morphology and histology of the male Syrian Golden hamster reproductive tract ductuli efferentes

Tue Jul 01 00:00:00 EDT 2014

Plant sterol feeding induces tumor formation and alters sterol metabolism in the intestine of Apc(Min) mice.

Nutrition and cancer

Marttinen M,Pajari AM,Päivärinta E,Storvik M,Marttinen P,Nurmi T,Niku M,Piironen V,Mutanen M

PA1-310B was used in western blot to assess how plant sterol esters alter intestinal tumorigenesis in adenomatous polyposis coli mice

Wed Oct 15 00:00:00 EDT 2014

Effects of in utero exposure to di(n-butyl) phthalate for estrogen receptors α, β, and androgen receptor of Leydig cell on rats.

Toxicologic pathology

Wakui S,Shirai M,Motohashi M,Mutou T,Oyama N,Wempe MF,Takahashi H,Inomata T,Ikegami M,Endou H,Asari M

PA1-310B was used in immunohistochemistry and western blot to study the expression of ER-alpha, ER-beta and AR in rat Leydig cells following in utero di(n-butyl) phthalate exposure

Tue Jul 01 00:00:00 EDT 2014

Estrogen, predominantly via estrogen receptor α, attenuates postpartum-induced anxiety- and depression-like behaviors in female rats.

Endocrinology

Furuta M,Numakawa T,Chiba S,Ninomiya M,Kajiyama Y,Adachi N,Akema T,Kunugi H

PA1-310B was used in immunohistochemistry - frozen section to study the effect of estrogen receptor subtypes to postpartum anxiogenic and depressive responses in rats

Tue Oct 01 00:00:00 EDT 2013

Chemoprevention of BBN-Induced Bladder Carcinogenesis by the Selective Estrogen Receptor Modulator Tamoxifen.

Translational oncology

George SK,Tovar-Sepulveda V,Shen SS,Jian W,Zhang Y,Hilsenbeck SG,Lerner SP,Smith CL

PA1-310B was used in immunohistochemistry to study the chemopreventative effects of tamoxifen in a murine model of carcinogen-induced bladder cancer

Sat Jun 01 00:00:00 EDT 2013

Age-dependent effects of prenatal stress on the corticolimbic dopaminergic system development in the rat male offspring.

Neurochemical research

Pallarés ME,Baier CJ,Adrover E,Monteleone MC,Brocco MA,Antonelli MC

PA1-310B was used in western blot to study the effects of pre-natal exposure to stress on the development of the corticolimbic dopaminergic system of male offspring in a rat model

Fri Nov 01 00:00:00 EDT 2013

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Clinical biochemistry

Kruer TL,Cummins TD,Powell DW,Wittliff JL

PA1-310B was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer

Fri Nov 01 00:00:00 EDT 2013

Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice.

Histochemistry and cell biology

Choijookhuu N,Sato Y,Nishino T,Endo D,Hishikawa Y,Koji T

PA1-310B was used in immunohistochemistry and western blot to study estrogen-dependent regulation of sodium/hydrogen exchanger-3 expression in the proximal colon of pregnant mice

Tue May 01 00:00:00 EDT 2012

Distribution of aromatase and sex steroid receptors in the baculum during the rat life cycle: effects of estrogen during the early development of the baculum.

Biology of reproduction

Yonezawa T,Higashi M,Yoshioka K,Mutoh K

PA1-310B was used in immunohistochemistry to investigate the role of aromatized estrogen in baculum development

Fri Jul 01 00:00:00 EDT 2011

Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice.

European journal of orthodontics

Márquez Hernández RA,Ohtani J,Fujita T,Sunagawa H,Kawata T,Kaku M,Motokawa M,Tanne K

PA1-310B was used in immunohistochemistry to investigate the effect of sex hormones receptors on bone development in neonatal mice

Sat Oct 01 00:00:00 EDT 2011

Cyclin D1/cdk4, estrogen receptors α and β, in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric carcinogenesis: immunohistochemical study.

The Journal of toxicological sciences

Motohashi M,Wakui S,Muto T,Suzuki Y,Shirai M,Takahashi H,Hano H

PA1-310B was used in immunohistochemistry to investigate the expression of cyclin D1/cdk4 and estrogen receptors in rat gastric carcinogenesis

Wed Jun 01 00:00:00 EDT 2011

Estrogen receptors in the central auditory system of male and female mice.

Neuroscience

Charitidi K,Canlon B

PA1-310B was used in immunohistochemistry to investigate the distribution of estrogen receptors alpha and beta in murine the central auditory system

Wed Feb 03 00:00:00 EST 2010

Dose-dependent effects of genistein and daidzein on protein metabolism in porcine myotube cultures.

Journal of agricultural and food chemistry

Rehfeldt C,Kalbe C,Nürnberg G,Mau M

PA1-310B was used in western blot to investigate the effect of isoflavones genistein and daidzein on skeletal muscle cell differentiation and protein metabolism

Wed Feb 11 00:00:00 EST 2009

Blockade of estrogen receptor signaling inhibits growth and migration of medulloblastoma.

Endocrinology

Belcher SM,Ma X,Le HH

PA1-310b was used in immunohistochemistry to study the role of estrogen receptor signaling in medulloblastoma development and migration.

Sun Mar 01 00:00:00 EST 2009

Estrogen promotes tumor progression in a genetically defined mouse model of lung adenocarcinoma.

Endocrine-related cancer

Hammoud Z,Tan B,Badve S,Bigsby RM

PA1-310B was used in western blot to use a genetically defined murine lung adenocarcinoma model to study the effects of estrogen on tumor progression

Sun Jun 01 00:00:00 EDT 2008

Estrogen receptors alpha and beta are inhibitory modifiers of Apc-dependent tumorigenesis in the proximal colon of Min/+ mice.

Cancer research

Cho NL,Javid SH,Carothers AM,Redston M,Bertagnolli MM

PA1-310B was used in immunohistochemistry to investigate the role of estrogen receptor beta during the inhibition of Apc-dependent colon tumorigenesis.

Thu Mar 01 00:00:00 EST 2007

Brain infusion of lipopolysaccharide increases uterine growth as a function of estrogen replacement regimen: suppression of uterine estrogen receptor-alpha by constant, but not pulsed, estrogen replacement.

Endocrinology

Marriott LK,McGann-Gramling KR,Hauss-Wegrzyniak B,Sheldahl LC,Shapiro RA,Dorsa DM,Wenk GL

PA1-310B was used in western blot to investigate the suppression of uterine estrogen receptor alpha caused by constant or pulsed estrogen replacement.

Mon Jan 01 00:00:00 EST 2007

Estrogen inhibition of norepinephrine responsiveness is initiated at the plasma membrane of GnRH-producing GT1-7 cells.

The Journal of endocrinology

Morales A,Gonzalez M,Marin R,Diaz M,Alonso R

PA1-310B was used in immunocytochemistry to investigate whether E2 is able to modify the response of GnRH-producing GT1-7 cells to norepinephrine (NE) stimulation

Sun Jul 01 00:00:00 EDT 2007

Estrogen replacement regimen and brain infusion of lipopolysaccharide differentially alter steroid receptor expression in the uterus and hypothalamus.

Endocrine

Marriott LK,McGann-Gramling KR,Hauss-Wegrzyniak B,Sheldahl LC,Shapiro RA,Dorsa DM,Wenk GL

PA1-310B was used in western blot to study the effects of estrogen replacement and lipopolysaccharide infusion on steroid receptor expression in the uterus and hypothalamus

Sat Dec 01 00:00:00 EST 2007

Distribution and localization patterns of estrogen receptor-beta and insulin-like growth factor-1 receptors in neurons and glial cells of the female rat substantia nigra: localization of ERbeta and IGF-1R in substantia nigra.

The Journal of comparative neurology

Quesada A,Romeo HE,Micevych P

PA1-310B was used in blocking/activating experiment to investigate the suggested neuroprotective functions of E2 and IGF-1 on SNpc DA neurons from an anaotomical view

Sun Jul 01 00:00:00 EDT 2007

Estradiol increases proteinuria and angiotensin II type 1 receptor in kidneys of rats receiving L-NAME and angiotensin II.

Kidney international

Oestreicher EM,Guo C,Seely EW,Kikuchi T,Martinez-Vasquez D,Jonasson L,Yao T,Burr D,Mayoral S,Roubsanthisuk W,Ricchiuti V,Adler GK

PA1-310B was used in immunocytochemistry to investigate the effect of E2 on the renovasculature in renal injury

Wed Nov 01 00:00:00 EST 2006

Gonadotrope oestrogen receptor-alpha and -beta and progesterone receptor immunoreactivity after ovariectomy and exposure to oestradiol benzoate, tamoxifen or raloxifene in the rat: correlation with LH secretion.

The Journal of endocrinology

Sánchez-Criado JE,de Las Mulas JM,Bellido C,Aguilar R,Garrido-Gracia JC

PA1-310B was used in immunohistochemistry to study the possible correlation between the immunohistochemical expression of ER isoforms in the gonadotrope in ovariectomized rats treated with oestradiol benzoate (EB), TX or raloxifene (RX) and LH secret

Sat Jan 01 00:00:00 EST 2005

Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Yamashita S,Okada Y

PA1-310B was used in immunohistochemistry to demonstrate the mechanisms of heat-induced antigen retrieval

Sat Jan 01 00:00:00 EST 2005

Oestrogen receptor beta-immunoreactive neurones in the ovine hypothalamus: distribution and colocalisation with gonadotropin-releasing hormone.

Journal of neuroendocrinology

Skinner DC,Dufourny L

PA1-310B was used in immunohistochemistry to investigate the distribution of estrogen receptor beta in hypothalamic neurons positive with gonadotropin-releasing hormone

Sat Jan 01 00:00:00 EST 2005

Genetic profiling of aortic allografts: prothymosin alpha as potential target?

Transplant international : official journal of the European Society for Organ Transplantation

Joosten SA,Smit van Dixhoorn MG,Borrias MC,Ham V,Groot Koerkamp MJ,Savolainen-Peltonen HM,Häyry P,Daha MR,Kooten C,Paul LC

PA1-310B was used in immunohistochemistry to identify genes potentially involved in transplant arteriosclerosis following aortic allograft

Mon Aug 01 00:00:00 EDT 2005

Non-nuclear estrogen receptor beta and alpha in the hippocampus of male and female rats.

Hippocampus

Kalita K,Szymczak S,Kaczmarek L

PA1-310B was used in immunocytochemistry to investigate the expression and subcellular localization of estrogen receptor beta in the rat hippocampus

Wed Jul 20 00:00:00 EDT 2005

The differential ability of the phytoestrogen genistein and of estradiol to induce uterine weight and proliferation in the rat is associated with a substance specific modulation of uterine gene expression.

Molecular and cellular endocrinology

Diel P,Geis RB,Caldarelli A,Schmidt S,Leschowsky UL,Voss A,Vollmer G

PA1-310B was used in immunohistochemistry to study the molecular mechanisms underlying the differential induction of rat uterine tissue proliferation by the plant phytoestrogen genistein and by estradiol

Wed Jun 30 00:00:00 EDT 2004

Prostate hyperplasia in a transgenic mouse with prostate-specific expression of prolactin.

Endocrinology

Kindblom J,Dillner K,Sahlin L,Robertson F,Ormandy C,Törnell J,Wennbo H

PA1-310B was used in immunohistochemistry to investigate the role of prolactin in the prostate hyperplasia.

Sun Jun 01 00:00:00 EDT 2003

Expression of functional estrogen receptor beta in locus coeruleus-derived Cath.a cells.

Endocrinology

Rincavage HL,McDonnell DP,Kuhn CM

PA1-310B was used in immunocytochemistry to study the expression of estrogen receptor beta in locus coeruleus-derivedcentral-adrenergic-tyrosine-hydroxylase-expressing cells.

Tue Jul 01 00:00:00 EDT 2003

Effect of estrogen and antiestrogens on the estrogen receptor content in the cochlea of ovariectomized rats.

Hearing research

Stenberg AE,Simonoska R,Stygar D,Sahlin L,Hultcrantz M

PA1-310B was used in immunohistochemistry to study the effect of estrogen and antiestrogens on the expression of estrogen receptor alpha and beta in the hearing organs

Fri Aug 01 00:00:00 EDT 2003

Cloning of the novel isoform of the estrogen receptor beta cDNA (ERbeta isoform M cDNA) from the human testicular cDNA library.

The Journal of steroid biochemistry and molecular biology

Shoda T,Hirata S,Kato J,Hoshi K

PA1-310B was used in western blot to identify and characterize a novel estrogen receptor beta cDNA isoform

Tue Oct 01 00:00:00 EDT 2002

Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression.

The Journal of endocrinology

Wiren KM,Chapman Evans A,Zhang XW

PA1-310B was used in western blot to investigate the effect of osteoblast differentiation on androgen and estrogen receptor-alpha and -beta expression

Sun Dec 01 00:00:00 EST 2002

ERbeta has nongenomic action in caveolae.

Molecular endocrinology (Baltimore, Md.)

Chambliss KL,Yuhanna IS,Anderson RG,Mendelsohn ME,Shaul PW

PA1-310B was used in western blot to determine whether ERbeta is localized to the endothelial cell plasma membrane

Wed May 01 00:00:00 EDT 2002

Expression of estrogen receptor-beta protein and mRNA in the cerebellum of the rat.

Neuroscience letters

Price RH,Handa RJ

PA1-310B was used in immunohistochemistry to determine the estrogen receptor beta protein expression in the rat cerebellum

Fri Jul 14 00:00:00 EDT 2000

Localization of estrogen receptor beta-immunoreactivity in astrocytes of the adult rat brain.

Glia

Azcoitia I,Sierra A,Garcia-Segura LM

PA1-310B was used in immunohistochemistry to investigate the distribution of ER-beta in astrocytes of rat brain

Sat May 01 00:00:00 EDT 1999

Generation and reproductive phenotypes of mice lacking estrogen receptor beta.

Proceedings of the National Academy of Sciences of the United States of America

Krege JH,Hodgin JB,Couse JF,Enmark E,Warner M,Mahler JF,Sar M,Korach KS,Gustafsson JA,Smithies O

PA1-310B was used in immunocytochemistry to study the role of the ERbeta during the ovulation

Tue Dec 22 00:00:00 EST 1998

Bioinformatics

Protein Aliases: ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta

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Gene Aliases: ER-BETA; ER[b]; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2

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UniProt ID: (Human) Q92731, (Mouse) O08537, (Rat) Q62986

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Entrez Gene ID: (Human) 2100, (Mouse) 13983, (Rat) 25149

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Function(s)

core promoter sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding drug binding zinc ion binding enzyme binding estrogen receptor activity estrogen response element binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding peroxisome proliferator activated receptor binding receptor antagonist activity estradiol binding steroid hormone binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding lipid binding hormone binding sequence-specific DNA binding metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Estrogen Receptor beta Polyclonal Antibody

Estrogen Receptor beta Antibody (PA1-310B) in ICC/IF

Product Details

PA1-310B

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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