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Invitrogen
Estrogen Receptor beta Recombinant Rabbit Monoclonal Antibody (JJ09-02)
Product Details
MA5-32471
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ09-02
Immunogen
Recombinant protein within Human Estrogen Receptor beta aa 440-530
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809748
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ER beta; ER-beta; estrogen receptor 2 (ER beta); Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2
Gene Aliases: ER-BETA; Erb; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
UniProt ID: (Human) Q92731
Entrez Gene ID: (Human) 2100
core promoter sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
drug binding
zinc ion binding
enzyme binding
estrogen receptor activity
estrogen response element binding
RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding
peroxisome proliferator activated receptor binding
receptor antagonist activity
estradiol binding
steroid hormone binding
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
behavioral fear response
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
signal transduction
cell-cell signaling
learning or memory
response to water deprivation
response to activity
response to insecticide
cerebellum development
amygdala development
hypothalamus development
negative regulation of cell growth
intracellular estrogen receptor signaling pathway
prostate gland development
response to nutrient levels
response to testosterone
response to genistein
cellular response to drug
positive regulation of apoptotic process
regulation of neuron apoptotic process
response to estrogen
response to human chorionic gonadotropin
estrous cycle
response to ethanol
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of smooth muscle cell proliferation
negative regulation of epithelial cell proliferation
positive regulation of sequence-specific DNA binding transcription factor activity
Sertoli cell development
Sertoli cell proliferation
positive regulation of ERK1 and ERK2 cascade
cellular response to magnetism
cellular response to estradiol stimulus
response to dexamethasone
extracellular negative regulation of signal transduction
negative regulation of neuron death
response to salt
response to bisphenol A
negative regulation of feeding behavior
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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