Proteintech

Ezrin Polyclonal Antibody

Ezrin Antibody in Immunocytochemistry (ICC/IF)

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Ezrin Antibody (26056-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 26056-1-AP (Ezrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Ezrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ezrin Antibody in Immunoprecipitation (IP)

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Product Details

26056-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:600

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Ezrin Fusion Protein Ag23530 (477-529 aa encoded by BC013903)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VYEPVSYHV QESLQDEGAE PTGYSAELSS EGIRDDRNEE KRITEAEKNE RVQR (477-529 aa encoded by B C013903)

Target Information

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.

Proceedings of the National Academy of Sciences of the United States of America

Khan K,Long B,Baleanu-Gogonea C,Gogonea V,Deshpande GM,Vasu K,Fox PL

Tue Feb 15 00:00:00 EST 2022

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Acta neuropathologica

Broadhead MJ,Bonthron C,Waddington J,Smith WV,Lopez MF,Burley S,Valli J,Zhu F,Komiyama NH,Smith C,Grant SGN,Miles GB

Fri Apr 01 00:00:00 EDT 2022

circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin.

Frontiers in oncology

Li X,Wang J,Long H,Lin W,Wang H,Chen Y,Yuan Q,Li X

Tue Apr 26 00:00:00 EDT 2022

circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin.

Frontiers in oncology

Li X,Wang J,Long H,Lin W,Wang H,Chen Y,Yuan Q,Li X

Tue Apr 26 00:00:00 EDT 2022

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

International journal of molecular sciences

Khan K,Long B,Deshpande GM,Fox PL

Mon Oct 19 00:00:00 EDT 2020

Role of mTOR signaling in the regulation of high glucose-induced podocyte injury.

Experimental and therapeutic medicine

Li Q,Zeng Y,Jiang Q,Wu C,Zhou J

Mon Apr 01 00:00:00 EDT 2019

Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.

The Journal of biological chemistry

Li S,Sato K,Gordon WC,Sendtner M,Bazan NG,Jin M

Fri Sep 28 00:00:00 EDT 2018

Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.

The Journal of biological chemistry

Li S,Sato K,Gordon WC,Sendtner M,Bazan NG,Jin M

Fri Sep 28 00:00:00 EDT 2018

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

eLife

Zhang DL,Sun YJ,Ma ML,Wang YJ,Lin H,Li RR,Liang ZL,Gao Y,Yang Z,He DF,Lin A,Mo H,Lu YJ,Li MJ,Kong W,Chung KY,Yi F,Li JY,Qin YY,Li J,Thomsen ARB,Kahsai AW,Chen ZJ,Xu ZG,Liu M,Li D,Yu X,Sun JP

Fri Feb 02 00:00:00 EST 2018

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

eLife

Zhang DL,Sun YJ,Ma ML,Wang YJ,Lin H,Li RR,Liang ZL,Gao Y,Yang Z,He DF,Lin A,Mo H,Lu YJ,Li MJ,Kong W,Chung KY,Yi F,Li JY,Qin YY,Li J,Thomsen ARB,Kahsai AW,Chen ZJ,Xu ZG,Liu M,Li D,Yu X,Sun JP

Fri Feb 02 00:00:00 EST 2018

Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma.

Medical science monitor : international medical journal of experimental and clinical research

Liu P,Yang P,Zhang Z,Liu M,Hu S

Mon Apr 09 00:00:00 EDT 2018

Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma.

Medical science monitor : international medical journal of experimental and clinical research

Liu P,Yang P,Zhang Z,Liu M,Hu S

Mon Apr 09 00:00:00 EDT 2018

Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery.

Scientific reports

Zhang H,Chen F,Fan X,Lin C,Hao Y,Wei H,Lin W,Jiang Y,He F

Tue Mar 21 00:00:00 EDT 2017

Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery.

Scientific reports

Zhang H,Chen F,Fan X,Lin C,Hao Y,Wei H,Lin W,Jiang Y,He F

Tue Mar 21 00:00:00 EDT 2017

Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression.

PloS one

Suchanski J,Tejchman A,Zacharski M,Piotrowska A,Grzegrzolka J,Chodaczek G,Nowinska K,Rys J,Dziegiel P,Kieda C,Ugorski M

Tue Oct 17 00:00:00 EDT 2017

Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression.

PloS one

Suchanski J,Tejchman A,Zacharski M,Piotrowska A,Grzegrzolka J,Chodaczek G,Nowinska K,Rys J,Dziegiel P,Kieda C,Ugorski M

Tue Oct 17 00:00:00 EDT 2017

Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression.

PloS one

Suchanski J,Tejchman A,Zacharski M,Piotrowska A,Grzegrzolka J,Chodaczek G,Nowinska K,Rys J,Dziegiel P,Kieda C,Ugorski M

Tue Oct 17 00:00:00 EDT 2017

Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression.

PloS one

Suchanski J,Tejchman A,Zacharski M,Piotrowska A,Grzegrzolka J,Chodaczek G,Nowinska K,Rys J,Dziegiel P,Kieda C,Ugorski M

Tue Oct 17 00:00:00 EDT 2017

Regulation of CD44 expression and focal adhesion by Golgi phosphatidylinositol 4-phosphate in breast cancer.

Cancer science

Ijuin T,Takeuchi Y,Shimono Y,Fukumoto M,Tokuda E,Takenawa T

Fri Jul 01 00:00:00 EDT 2016

Regulation of CD44 expression and focal adhesion by Golgi phosphatidylinositol 4-phosphate in breast cancer.

Cancer science

Ijuin T,Takeuchi Y,Shimono Y,Fukumoto M,Tokuda E,Takenawa T

Fri Jul 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; EZRI; Ezrin; FLJ26216; MGC1584; p81; villin 2; villin 2 (ezrin); Villin-2

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Gene Aliases: AW146364; CVIL; CVL; EZR; HEL-S-105; p81; R75297; VIL2

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UniProt ID: (Human) P15311, (Mouse) P26040

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Entrez Gene ID: (Human) 7430, (Mouse) 22350

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Function(s)

actin binding protein binding microtubule binding protein domain specific binding protein kinase A catalytic subunit binding protein kinase A regulatory subunit binding S100 protein binding poly(A) RNA binding cell adhesion molecule binding actin filament binding ATPase binding cadherin binding involved in cell-cell adhesion cytoskeletal protein binding protein complex binding actin or actin-binding cytoskeletal protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter intestinal D-glucose absorption sphingosine-1-phosphate signaling pathway cytoskeletal anchoring at plasma membrane leukocyte cell-cell adhesion axon guidance regulation of cell shape regulation of cell size positive regulation of gene expression protein kinase A signaling gland morphogenesis membrane to membrane docking microvillus assembly astral microtubule organization actin cytoskeleton reorganization receptor internalization regulation of microvillus length regulation of actin cytoskeleton organization cellular protein complex localization positive regulation of multicellular organism growth cortical microtubule organization establishment of epithelial cell apical/basal polarity filopodium assembly phosphatidylinositol-mediated signaling positive regulation of protein secretion negative regulation of T cell receptor signaling pathway actin filament bundle assembly establishment of centrosome localization establishment of endothelial barrier negative regulation of ERK1 and ERK2 cascade cellular response to cAMP protein localization to cell cortex establishment of protein localization to plasma membrane positive regulation of establishment of protein localization to plasma membrane negative regulation of interleukin-2 secretion regulation of NIK/NF-kappaB signaling regulation of organelle assembly terminal web assembly positive regulation of protein localization to early endosome positive regulation of cellular protein catabolic process negative regulation of p38MAPK cascade positive regulation of early endosome to late endosome transport establishment or maintenance of apical/basal cell polarity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ezrin Polyclonal Antibody

Ezrin Antibody (26056-1-AP) in ICC/IF

Product Details

26056-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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