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Ezrin Antibody (PA5-17767) in WB
Western blot was performed using Anti-Ezrin Polyclonal Antibody(Product # PA5-17767) and a ~70kDa band corresponding to Ezrin was observed across cell lines tested . Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), HCT 116 (Lane 2), A-431 (Lane 3), HaCaT (Lane 4), NTERA-2 cl.D1 (Lane 5), HEK-293 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). ... View More
Product Details
PA5-17767
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues surrounding Thr567 of human ezrin
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11005047
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with related proteins such as merlin or band 4.
Target Information
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; EZRI; Ezrin; FLJ26216; MGC1584; p81; villin 2; villin 2 (ezrin); Villin-2
Gene Aliases: AW146364; CVIL; CVL; EZR; ezr.S; HEL-S-105; p81; R75297; VIL2; XELAEV_18027043mg
UniProt ID: (Bovine) P31976, (Human) P15311, (Mouse) P26040, (Rat) P31977
Entrez Gene ID: (Bovine) 281574, (Human) 7430, (Dog) 484056, (Mouse) 22350, (Rat) 54319, (Xenopus) 447216
cell adhesion molecule binding
actin filament binding
actin binding
protein binding
microtubule binding
protein domain specific binding
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
S100 protein binding
poly(A) RNA binding
ATPase binding
cadherin binding involved in cell-cell adhesion
cytoskeletal protein binding
protein complex binding
structural molecule activity
actin or actin-binding cytoskeletal protein
regulation of cell shape
establishment or maintenance of apical/basal cell polarity
actin filament bundle assembly
negative regulation of transcription from RNA polymerase II promoter
intestinal D-glucose absorption
sphingosine-1-phosphate signaling pathway
cytoskeletal anchoring at plasma membrane
leukocyte cell-cell adhesion
axon guidance
regulation of cell size
positive regulation of gene expression
protein kinase A signaling
gland morphogenesis
membrane to membrane docking
microvillus assembly
astral microtubule organization
actin cytoskeleton reorganization
receptor internalization
regulation of microvillus length
regulation of actin cytoskeleton organization
cellular protein complex localization
positive regulation of multicellular organism growth
cortical microtubule organization
establishment of epithelial cell apical/basal polarity
filopodium assembly
phosphatidylinositol-mediated signaling
positive regulation of protein secretion
negative regulation of T cell receptor signaling pathway
establishment of centrosome localization
establishment of endothelial barrier
negative regulation of ERK1 and ERK2 cascade
cellular response to cAMP
protein localization to cell cortex
establishment of protein localization to plasma membrane
positive regulation of establishment of protein localization to plasma membrane
negative regulation of interleukin-2 secretion
regulation of NIK/NF-kappaB signaling
regulation of organelle assembly
terminal web assembly
positive regulation of protein localization to early endosome
positive regulation of cellular protein catabolic process
negative regulation of p38MAPK cascade
positive regulation of early endosome to late endosome transport
epithelial cell differentiation
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