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Invitrogen
F4/80 Monoclonal Antibody (Cl:A3-1)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
F4/80 Antibody (MA5-16630) in ICC/IF
Immunofluorescence analysis of F4/80 was performed using 70% confluent log phase RAW 264.7 and NMuMG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with F4/80 Monoclonal Antibody (CI:A3-1) (Product # MA5-16632) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006)... View More
Product Details
MA5-16630
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
Cl:A3-1
Immunogen
Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538126
Product Specific Information
This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hrs. Citrate buffer (pH 6.0) is recommended for tissues fixed for more than 24 hrs. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71
Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3
UniProt ID: (Mouse) Q61549
Entrez Gene ID: (Mouse) 13733
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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