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FA2H Polyclonal Antibody

FA2H Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

FA2H Antibody (15452-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15452-1-AP (FA2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FA2H Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15452-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FA2H Fusion Protein Ag7717 (1-164 aa encoded by BC002679)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAPAPPPAA SFSPSEVQRR LAAGACWVRR GARLYDLSSF VRHHPGGEQL LRARAGQDIS ADLDGPPHRH SANARRWLEQ YYVGELRGEQ QGSMENEPVA LEETQKTDPA MEPRFKVVDW DKDLVDWRKP LLWQVGHLGE KYDEWVHQPV TRPIRLFHSD LIEGL (1-164 aa encoded by BC002679)

Target Information

FA2H is a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy.

Developmental cell

Xie XP,Laks DR,Sun D,Ganbold M,Wang Z,Pedraza AM,Bale T,Tabar V,Brennan C,Zhou X,Parada LF

Mon Jan 10 00:00:00 EST 2022

Dysregulated ceramides metabolism by fatty acid 2-hydroxylase exposes a metabolic vulnerability to target cancer metastasis.

Signal transduction and targeted therapy

Zhou X,Huang F,Ma G,Wei W,Wu N,Liu Z

Mon Oct 24 00:00:00 EDT 2022

Perfluorooctanoic acid (PFOA) as a stimulator of estrogen receptor-negative breast cancer MDA-MB-231 cell aggressiveness: Evidence for involvement of fatty acid 2-hydroxylase (FA2H) in the stimulated cell migration.

The Journal of toxicological sciences

Sakai G,Hirao-Suzuki M,Koga T,Kobayashi T,Kamishikiryo J,Tanaka M,Fujii K,Takiguchi M,Sugihara N,Toda A,Takeda S

Tue Apr 05 00:00:00 EDT 2022

Cell Lineage-Based Stratification for Glioblastoma.

Cancer cell

Wang Z,Sun D,Chen YJ,Xie X,Shi Y,Tabar V,Brennan CW,Bale TA,Jayewickreme CD,Laks DR,Alcantara Llaguno S,Parada LF

Mon Sep 14 00:00:00 EDT 2020

Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration.

Biochemical and biophysical research communications

Hirao-Suzuki M,Koga T,Sakai G,Kobayashi T,Ishii Y,Miyazawa H,Takiguchi M,Sugihara N,Toda A,Ohara M,Takeda S

Thu Oct 15 00:00:00 EDT 2020

FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control.

Frontiers in oncology

Dai X,Zhang S,Cheng H,Cai D,Chen X,Huang Z

Sat Oct 14 00:00:00 EDT 2023

FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control.

Frontiers in oncology

Dai X,Zhang S,Cheng H,Cai D,Chen X,Huang Z

Sat Oct 14 00:00:00 EDT 2023

Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.

Journal of medicinal chemistry

Baguley TD,Xu HC,Chatterjee M,Nairn AC,Lombroso PJ,Ellman JA

Thu Oct 10 00:00:00 EDT 2013

Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.

Journal of medicinal chemistry

Baguley TD,Xu HC,Chatterjee M,Nairn AC,Lombroso PJ,Ellman JA

Thu Oct 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Fatty acid 2-hydroxylase; Fatty acid alpha-hydroxylase; fatty acid hydroxylase domain containing 1; Fatty acid hydroxylase domain-containing protein 1; FLJ25287; spastic paraplegia 35 (autosomal recessive); WD repeat domain 59

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Gene Aliases: FA2H; FAAH; FAH1; FAXDC1; G630055L08Rik; RGD1310347; SCS7; SPG35; Wdr59

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UniProt ID: (Human) Q7L5A8, (Rat) Q2LAM0, (Mouse) Q5MPP0

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Entrez Gene ID: (Human) 79152, (Rat) 307855, (Mouse) 338521

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Function(s)

iron ion binding heme binding fatty acid alpha-hydroxylase activity N-ethylmaleimide reductase activity oxidoreductase activity reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity metal ion binding epoxyqueuosine reductase activity hydroxylase

Process(es)

sebaceous gland cell differentiation fatty acid metabolic process fatty acid biosynthetic process sphingolipid biosynthetic process lipid modification central nervous system myelin maintenance peripheral nervous system myelin maintenance regulation of cell proliferation regulation of hair cycle oxidation-reduction process sphingolipid metabolic process lipid metabolic process transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FA2H Polyclonal Antibody

FA2H Antibody (15452-1-AP) in ICC/IF

Product Details

15452-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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