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FIGURE: 1 / 3
FADD Antibody (MA5-52558) in ICC/IF
Immunocytochemistry analysis of FADD in C2C12 cells. Nuclei were stained blue with DAPI; FADD recombinant monoclonal antibody (Product # MA5-52558) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-52558
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB3590
Immunogen
A synthetic peptide of human FADD (150-205AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; Fas-associating protein with death domain; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; protein FADD
Gene Aliases: FADD; GIG3; MORT1; Mort1/FADD
UniProt ID: (Human) Q13158, (Mouse) Q61160
Entrez Gene ID: (Human) 8772, (Mouse) 14082, (Rat) 266610
positive regulation of T cell mediated cytotoxicity
immune system process
positive regulation of adaptive immune response
apoptotic process
signal transduction
T cell differentiation
positive regulation of interferon-gamma production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
T cell differentiation in thymus
TRAIL-activated apoptotic signaling pathway
positive regulation of activated T cell proliferation
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
innate immune response
positive regulation of macrophage differentiation
positive regulation of proteolysis
positive regulation of transcription from RNA polymerase II promoter
lymph node development
spleen development
thymus development
cardiac muscle tissue development
protein heterooligomerization
defense response to virus
positive regulation of type I interferon-mediated signaling pathway
negative regulation of necroptotic process
negative regulation of activation-induced cell death of T cells
motor neuron apoptotic process
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
activation of cysteine-type endopeptidase activity
necroptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation
positive regulation of extrinsic apoptotic signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
cellular response to mechanical stimulus
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell surface receptor signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
TRIF-dependent toll-like receptor signaling pathway
death-inducing signaling complex assembly
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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