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Product Details
AMO0672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein containing the C-terminal 150 amino acid sequence of mouse FAK expressed in bacteria.
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
RRID
AB_2536399
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.10.2; FADK 1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; Protein-tyrosine kinase 2
Gene Aliases: Fadk; FAK; Fak1; FRNK; Kiaa4203; mKIAA4203; p125FAK; Ptk2
UniProt ID: (Mouse) Q5DTH7
Entrez Gene ID: (Mouse) 14083
nucleotide binding
actin binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
signal transducer activity
receptor binding
integrin binding
protein binding
ATP binding
JUN kinase binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
protein complex binding
SH2 domain binding
identical protein binding
phosphatidylinositol 3-kinase binding
MAPK cascade
microtubule cytoskeleton organization
angiogenesis
blood vessel development
vasculogenesis
neuron migration
regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein phosphorylation
movement of cell or subcellular component
cell adhesion
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
JNK cascade
multicellular organism development
positive regulation of cell proliferation
regulation of cell shape
regulation of epithelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of smooth muscle cell migration
phosphorylation
peptidyl-tyrosine phosphorylation
central nervous system neuron axonogenesis
negative regulation of cell-cell adhesion
regulation of cell adhesion
extracellular matrix organization
positive regulation of cell growth
positive regulation of cell migration
negative regulation of cell migration
cellular chloride ion homeostasis
regulation of cell adhesion mediated by integrin
peptidyl-tyrosine autophosphorylation
establishment of nucleus localization
regulation of cell proliferation
negative regulation of apoptotic process
endothelial cell migration
innate immune response
regulation of osteoblast differentiation
positive regulation of cell adhesion
positive regulation of protein kinase activity
positive regulation of vasodilation
negative regulation of organ growth
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of smooth muscle cell proliferation
positive regulation of phagocytosis
negative regulation of axonogenesis
positive regulation of synaptic transmission
regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of synapse assembly
positive regulation of glial cell proliferation
growth hormone receptor signaling pathway
cellular response to transforming growth factor beta stimulus
regulation of substrate adhesion-dependent cell spreading
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of anoikis
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