Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA5-15644
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10H7E9
Immunogen
Purified recombinant fragment of human FAK expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10987038
Product Specific Information
MA5-15644 targets FAK in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human samples.
The MA5-15644 immunogen is purified recombinant fragment of human FAK expressed in E. Coli.
.
MA5-15644 detects FAK which has a predicted molecular weight of approximately 119kDa.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2
Gene Aliases: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2
UniProt ID: (Human) Q05397
Entrez Gene ID: (Human) 5747
actin binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
protein binding
ATP binding
JUN kinase binding
protein kinase binding
SH2 domain binding
MAPK cascade
microtubule cytoskeleton organization
angiogenesis
vasculogenesis
neuron migration
placenta development
regulation of protein phosphorylation
positive regulation of protein phosphorylation
heart morphogenesis
cellular component disassembly involved in execution phase of apoptosis
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
axon guidance
positive regulation of cell proliferation
regulation of cell shape
embryo development
regulation of endothelial cell migration
regulation of epithelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
central nervous system neuron axonogenesis
negative regulation of cell-cell adhesion
establishment of cell polarity
extracellular matrix organization
positive regulation of cell migration
regulation of cell adhesion mediated by integrin
netrin-activated signaling pathway
peptidyl-tyrosine autophosphorylation
Fc-gamma receptor signaling pathway involved in phagocytosis
establishment of nucleus localization
regulation of cell proliferation
negative regulation of apoptotic process
regulation of GTPase activity
endothelial cell migration
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
innate immune response
regulation of osteoblast differentiation
positive regulation of protein kinase activity
negative regulation of organ growth
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
cell motility
negative regulation of axonogenesis
regulation of cytoskeleton organization
regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of synapse assembly
growth hormone receptor signaling pathway
regulation of substrate adhesion-dependent cell spreading
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of anoikis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support