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FAK Monoclonal Antibody (2C5B9)

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Antibody Testing Data (10)

FAK Antibody in Immunocytochemistry (ICC/IF)

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FAK Antibody (66258-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (Product # 66258-1-IG, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

FAK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66258-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2c

Class

Monoclonal

Type

Antibody

Clone

2C5B9

Immunogen

FAK Fusion Protein Ag17966 (381-678 aa encoded by BC028733)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.43 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ITAMAGSIY PGQASLLDQT DSWNHRPQEI AMWQPNVEDS TVLDLRGIGQ VLPTHLMEER LIRQQQEMEE DQRWLEKEER FLKPDVRLSR GSIDREDGSL QGPIGNQHIY QPVGKPDPAA PPKKPPRPGA PGHLGSLASL SSPADSYNEG VKLKPQEISP PPTANLDRSN DKVYENVTGL VKAVIEMSSK IQPAPPEEYV PMVKEVGLAL RTLLATVDET IPLLPASTHR EIEMAQKLLN SDLGELINKM KLAQQYVMTS LQQEYKKQML TAAHALAVDA KNLLDVIDQA RLKMLGQTR (381-678 aa encoded by BC028733)

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT Signaling.

Stem cells international

He WG,Deng YX,Ke KX,Cao XL,Liu SY,Yang YY,Luo HH,Yao XT,Gao X,Du Y,He BC,Chen L

Thu Feb 02 00:00:00 EST 2023

Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats.

Life science alliance

Pang S,Luo Z,Dong W,Gao S,Chen W,Liu N,Zhang X,Gao X,Li J,Gao K,Shi X,Guan F,Zhang L,Zhang L

Wed Mar 01 00:00:00 EST 2023

Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway.

Frontiers in pharmacology

Zhang MX,Huang XY,Song Y,Xu WL,Li YL,Li C

Thu Feb 02 00:00:00 EST 2023

AVL9 promotes colorectal carcinoma cell migration via regulating EGFR expression.

Biological procedures online

Wu Q,Chen J,Zhou Z

Thu Jan 06 00:00:00 EST 2022

TNFSF9 promotes metastasis of pancreatic cancer by regulating M2 polarization of macrophages through Src/FAK/p-Akt/IL-1β signaling.

International immunopharmacology

Wu J,Wang Y,Yang Y,Liu F,Jiang Z,Jiang Z

Sat Jan 01 00:00:00 EST 2022

LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway.

BMC cancer

Tan Y,Chen Q,Pan S,An W,Xu H,Xing Y,Zhang J

Sat Apr 30 00:00:00 EDT 2022

Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.

Cell reports

Hadjisavva R,Anastasiou O,Ioannou PS,Zheltkova M,Skourides PA

Tue Jul 19 00:00:00 EDT 2022

LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/β-catenin signaling.

Journal of cell science

Liu W,Jiang X,Li X,Sun K,Yang Y,Yang M,Li S,Zhu X

Tue Mar 15 00:00:00 EDT 2022

Src kinase mediates coelomocytes phagocytosis via interacting with focal adhesion kinase in Vibrio splendidus challenged Apostichopus japonicus.

Fish & shellfish immunology

Wan J,Zhao X,Liu J,Chen K,Li C

Sun May 01 00:00:00 EDT 2022

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.

Oncogene

Ma RR,Zhang H,Chen HF,Zhang GH,Tian YR,Gao P

Thu Apr 01 00:00:00 EDT 2021

MT1-MMP-dependent ECM processing regulates laminB1 stability and mediates replication fork restart.

PloS one

Thakur V,Tiburcio de Freitas J,Li Y,Zhang K,Savadelis A,Bedogni B

Tue Nov 02 00:00:00 EDT 2021

Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.

Cell death & disease

Liu H,Zhou Y,Qiu H,Zhuang R,Han Y,Liu X,Qiu X,Wang Z,Xu L,Tan R,Hong W,Wang T

Wed Mar 17 00:00:00 EDT 2021

NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer.

Biochemical and biophysical research communications

Lin F,Zhou J,Li X,Wang X

Fri Jun 25 00:00:00 EDT 2021

CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.

Journal of Cancer

Li L,Tao X,Li Y,Gao Y,Li Q

Sat Nov 11 00:00:00 EST 2023

TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.

Aging

Wu J,Wang Y,Yang Y,Liu F,Chen J,Jiang Z,Jiang Z

Mon Sep 13 00:00:00 EDT 2021

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Acta biomaterialia

Ma S,Zhou J,Huang T,Zhang Z,Xing Q,Zhou X,Zhang K,Yao M,Cheng T,Wang X,Wen X,Guan F

Wed Sep 01 00:00:00 EDT 2021

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Cell communication and signaling : CCS

Antoniades I,Kyriakou M,Charalambous A,Kalalidou K,Christodoulou A,Christoforou M,Skourides PA

Thu Jan 07 00:00:00 EST 2021

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Cell communication and signaling : CCS

Antoniades I,Kyriakou M,Charalambous A,Kalalidou K,Christodoulou A,Christoforou M,Skourides PA

Thu Jan 07 00:00:00 EST 2021

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Cell communication and signaling : CCS

Antoniades I,Kyriakou M,Charalambous A,Kalalidou K,Christodoulou A,Christoforou M,Skourides PA

Thu Jan 07 00:00:00 EST 2021

DHPAC, a novel microtubule depolymerizing agent, suppresses angiogenesis and vasculogenic mimicry formation of human non-small cell lung cancer.

Journal of cellular biochemistry

Gong FL,Wang L,Yu LG,Dang YF,Jiang XN,Zhao L,Guo XL

Tue Dec 01 00:00:00 EST 2020

RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression.

Molecular medicine reports

Yu S,Gautam N,Quan M,Gao Y

Thu Aug 01 00:00:00 EDT 2019

Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer.

Biology open

Wang G,Yang Q,Li M,Zhang Y,Cai Y,Liang X,Fu Y,Xiao Z,Zhou M,Xie Z,Huang H,Huang Y,Chen Y,He Q,Peng F,Chen Z

Mon May 13 00:00:00 EDT 2019

TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.

Inflammation

Wu X,Li S,Wang K,Hua W,Li S,Song Y,Zhang Y,Yang S,Yang C

Tue Oct 01 00:00:00 EDT 2019

Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.

Cells

Ritter A,Friemel A,Roth S,Kreis NN,Hoock SC,Safdar BK,Fischer K,Möllmann C,Solbach C,Louwen F,Yuan J

Mon Oct 21 00:00:00 EDT 2019

AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.

Journal of cellular and molecular medicine

Chen ZL,Yang J,Shen YW,Li ST,Wang X,Lv M,Wang BY,Li P,Zhao W,Qiu RY,Liu Y,Liu PJ,Yang J

Sun Apr 01 00:00:00 EDT 2018

AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.

Journal of cellular and molecular medicine

Chen ZL,Yang J,Shen YW,Li ST,Wang X,Lv M,Wang BY,Li P,Zhao W,Qiu RY,Liu Y,Liu PJ,Yang J

Sun Apr 01 00:00:00 EDT 2018

AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.

Journal of cellular and molecular medicine

Chen ZL,Yang J,Shen YW,Li ST,Wang X,Lv M,Wang BY,Li P,Zhao W,Qiu RY,Liu Y,Liu PJ,Yang J

Sun Apr 01 00:00:00 EDT 2018

AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.

Journal of cellular and molecular medicine

Chen ZL,Yang J,Shen YW,Li ST,Wang X,Lv M,Wang BY,Li P,Zhao W,Qiu RY,Liu Y,Liu PJ,Yang J

Sun Apr 01 00:00:00 EDT 2018

Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level.

IUBMB life

Wu Q,Yu S,Chen J,Li Y,Gao Y

Thu Feb 01 00:00:00 EST 2018

Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level.

IUBMB life

Wu Q,Yu S,Chen J,Li Y,Gao Y

Thu Feb 01 00:00:00 EST 2018

Rab34 regulates adhesion, migration, and invasion of breast cancer cells.

Oncogene

Sun L,Xu X,Chen Y,Zhou Y,Tan R,Qiu H,Jin L,Zhang W,Fan R,Hong W,Wang T

Sun Jul 01 00:00:00 EDT 2018

Downregulation of Talin1 promotes hepatocellular carcinoma progression through activation of the ERK1/2 pathway.

Cancer science

Chen P,Lei L,Wang J,Zou X,Zhang D,Deng L,Wu D

Thu Jun 01 00:00:00 EDT 2017

Downregulation of Talin1 promotes hepatocellular carcinoma progression through activation of the ERK1/2 pathway.

Cancer science

Chen P,Lei L,Wang J,Zou X,Zhang D,Deng L,Wu D

Thu Jun 01 00:00:00 EDT 2017

Vascular protective effects of aqueous extracts of Tribulus terrestris on hypertensive endothelial injury.

Chinese journal of natural medicines

Jiang YH,Guo JH,Wu S,Yang CH

Tue Aug 01 00:00:00 EDT 2017

β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.

The Journal of biological chemistry

Alekhina O,Marchese A

Fri Dec 09 00:00:00 EST 2016

β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.

The Journal of biological chemistry

Alekhina O,Marchese A

Fri Dec 09 00:00:00 EST 2016

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FAK Monoclonal Antibody (2C5B9)

FAK Antibody (66258-1-IG) in ICC/IF

Product Details

66258-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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