Invitrogen

FAK Monoclonal Antibody (34Q36)

Advanced Verification

IP-mass spectrometry

3 Published Figures

6 References

View all (102) FAK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FAK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

FAK Antibody (AHO1272) in ICC/IF

Immunofluorescent analysis of FAK was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with FAK Mouse Monoclonal Antibody (Product # AHO1272) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30... View More

FAK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAK Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

FAK Antibody — IP-MS enrichment of FAK (LFQ intensity): FAK was enriched 290-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and FAK antibody (Product # AHO1272). See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

AHO1272

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

34Q36

Immunogen

Recombinant fragment of human FAK expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536330

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peruvoside is a novel Src inhibitor that suppresses NSCLC cell growth and motility by downregulating multiple Src-EGFR-related pathways.

American journal of cancer research

Lai Y,Chang H,Chen H,Chang G,Chen JJ

AHO1272 was used in Western Blotting to suggest that peruvoside suppresses non-small-cell lung carcinoma (NSCLC) malignancy by downregulating multiple Src-related pathways and could serve as a potential base molecule for developing new anticancer drugs and therapeutic strategies for lung cancer.

Sat Jul 16 00:00:00 EDT 2022

Identification of TENM4 as a Novel Cancer Stem Cell-Associated Molecule and Potential Target in Triple Negative Breast Cancer.

Cancers

Ruiu R,Barutello G,Arigoni M,Riccardo F,Conti L,Peppino G,Annaratone L,Marchiò C,Mengozzi G,Calogero RA,Cavallo F,Quaglino E

AHO1272 was used in Western Blotting to indicate that TENM4 may act as a novel biomarker and target for the treatment of TNBC.

Sat Feb 20 00:00:00 EST 2021

Identification of TENM4 as a Novel Cancer Stem Cell-Associated Molecule and Potential Target in Triple Negative Breast Cancer.

Cancers

Ruiu R,Barutello G,Arigoni M,Riccardo F,Conti L,Peppino G,Annaratone L,Marchiò C,Mengozzi G,Calogero RA,Cavallo F,Quaglino E

AHO1272 was used in Western Blotting to indicate that TENM4 may act as a novel biomarker and target for the treatment of TNBC.

Sat Feb 20 00:00:00 EST 2021

Human brain microvascular endothelial cell pairs model tissue-level blood-brain barrier function.

Integrative biology : quantitative biosciences from nano to macro

O'Connor BB,Grevesse T,Zimmerman JF,Ardoña HAM,Jimenez JA,Bitounis D,Demokritou P,Parker KK

AHO1272 was used in Simple Western - Size to predict blood-brain barrier permeability states based on structural changes in human brain endothelial cells.

Tue Apr 14 00:00:00 EDT 2020

Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling.

Life science alliance

Bersini S,Lytle NK,Schulte R,Huang L,Wahl GM,Hetzer MW

Published figure using FAK monoclonal antibody (Product # AHO1272) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling.

Life science alliance

Bersini S,Lytle NK,Schulte R,Huang L,Wahl GM,Hetzer MW

Published figure using FAK monoclonal antibody (Product # AHO1272) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

Tumor endothelial cells promote metastasis and cancer stem cell-like phenotype through elevated Epiregulin in esophageal cancer.

American journal of cancer research

Sun L,Pan J,Yu L,Liu H,Shu X,Sun L,Lou J,Yang Z,Ran Y

AHO1272 was used in western blot to investigate the molecular mechanisms by which human esophageal cancer endothelial cells contribute to esophageal carcinoma progression

Wed Mar 16 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FAK Monoclonal Antibody (34Q36)

FAK Antibody (AHO1272) in ICC/IF

Product Details

AHO1272

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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