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FALZ Antibody (MA5-15842) in ICC/IF
Immunofluorescent analysis of BDTF (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a BDTF monoclonal antibody (Product # MA5-15842) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1... View More
Product Details
MA5-15842
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2F10
Immunogen
Purified recombinant fragment of human BPTF expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11154265
Product Specific Information
MA5-15842 has been tested in indirect ELISA.
Target Information
BPTF (bromodomain and PHD domain transcription factor) is the largest subunit of the ATP-dependent chromatin-remodelling complex, NURF (nucleosome remodelling factor). NURF catalyses ATP-dependent nucleosome sliding and facilitates transcription. BPTF recognises histone H3 tails that are tri-methylated at K4, which marks the transcriptional start site of the vast majority of transcriptionally active genes. BPTF also exhibits some binding to H3 di-methylated at K4. BPTF plays a key role in the development of early mouse embryos, possibly through regulation of the Smad pathway of transcription factors. While BPTF is expressed in low levels in the adult brain and spinal cord, it is expressed in higher levels in the brain in neurodegenerative diseases. It is present in a subset of amyloid-containing plaques in the brains of patients suffering from Alzheimer's disease. Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).Tissue specificity: Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bromodomain and PHD domain transcription factor; Bromodomain and PHD finger-containing transcription factor; Fetal Alz-50 clone 1 protein; fetal Alz-50 reactive clone 1; Fetal Alzheimer antigen; nucleosome remodeling factor, large subunit; Nucleosome-remodeling factor subunit BPTF
Gene Aliases: BPTF; FAC1; FALZ; NURF301
UniProt ID: (Human) Q12830
Entrez Gene ID: (Human) 2186
negative regulation of transcription from RNA polymerase II promoter
embryonic placenta development
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
brain development
endoderm development
response to wounding
anterior/posterior pattern specification
covalent chromatin modification
positive regulation of transcription, DNA-templated
cellular response to nerve growth factor stimulus
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