Proteintech

FAS/CD95 Monoclonal Antibody (1G7F7)

FAS/CD95 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FAS/CD95 Antibody (60196-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FAS/CD95 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

60196-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G7F7

Immunogen

FAS/CD95 Fusion Protein Ag3718 (156-335 aa encoded by BC012479)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.13 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.

Immunogen sequence: ECTLTSNTK CKEEGSRSNL GWLCLLLLPI PLIVWVKRKE VQKTCRKHRK ENQGSHESPT LNPETVAINL SDVDLSKYIT TIAGVMTLSQ VKGFVRKNGV NEAKIDEIKN DNVQDTAEQK VQLLRNWHQL HGKKEAYDTL IKDLKKANLC TLAEKIQTII LKDITSDSEN SNFRNEIQSL V (156-335 aa encoded by BC012479)

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A novel role of Fas in delaying cellular senescence.

Heliyon

Saha C,Li J,Sun X,Liu X,Huang G

Wed Feb 01 00:00:00 EST 2023

Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.

Food & function

Tao W,Cao W,Yu B,Chen H,Gong R,Luorong Q,Luo J,Yao L,Zhang D

Mon Jun 06 00:00:00 EDT 2022

Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.

Food & function

Tao W,Cao W,Yu B,Chen H,Gong R,Luorong Q,Luo J,Yao L,Zhang D

Mon Jun 06 00:00:00 EDT 2022

Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.

Cell

Cui C,Chakraborty K,Tang XA,Zhou G,Schoenfelt KQ,Becker KM,Hoffman A,Chang YF,Blank A,Reardon CA,Kenny HA,Vaisar T,Lengyel E,Greene G,Becker L

Thu Jun 10 00:00:00 EDT 2021

GRHL2 Upregulation Predicts a Poor Prognosis and Promotes the Resistance of Serous Ovarian Cancer to Cisplatin.

OncoTargets and therapy

Nie Y,Ding Y,Yang M

Fri Apr 15 00:00:00 EDT 2022

Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos.

Chemosphere

Jin M,Xiao Z,Zhang S,Men X,Li X,Zhang B,Zhou T,Hsiao CD,Liu K

Fri Mar 01 00:00:00 EST 2019

Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos.

Chemosphere

Jin M,Xiao Z,Zhang S,Men X,Li X,Zhang B,Zhou T,Hsiao CD,Liu K

Fri Mar 01 00:00:00 EST 2019

Restoration of p53 acetylation by HDAC inhibition permits the necrosis/apoptosis switch of pancreatic ainar cell during experimental pancreatitis in mice.

Journal of cellular physiology

Hu Y,Dai J,Zong G,Xiao J,Guo X,Dai Y,Lu Z,Wan R

Sun Dec 01 00:00:00 EST 2019

Loss of Adipose Growth Hormone Receptor in Mice Enhances Local Fatty Acid Trapping and Impairs Brown Adipose Tissue Thermogenesis.

iScience

Ran L,Wang X,Mi A,Liu Y,Wu J,Wang H,Guo M,Sun J,Liu B,Li Y,Wang D,Jiang R,Wang N,Gao W,Zeng L,Huang L,Chen X,LeRoith D,Liang B,Li X,Wu Y

Fri Jun 28 00:00:00 EDT 2019

miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.

Journal of physiology and biochemistry

Chen C,Deng Y,Hu X,Ren H,Zhu J,Fu S,Xie J,Peng Y

Wed Aug 01 00:00:00 EDT 2018

miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.

Journal of physiology and biochemistry

Chen C,Deng Y,Hu X,Ren H,Zhu J,Fu S,Xie J,Peng Y

Wed Aug 01 00:00:00 EDT 2018

The protective role of human ghrelin in sepsis: Restoration of CD4 T cell proliferation.

PloS one

Zhou M,Aziz M,Ochani M,Yang WL,Sharma A,Wang P

Tue Jan 22 00:00:00 EST 2019

The protective role of human ghrelin in sepsis: Restoration of CD4 T cell proliferation.

PloS one

Zhou M,Aziz M,Ochani M,Yang WL,Sharma A,Wang P

Tue Jan 22 00:00:00 EST 2019

Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways.

Biochimica et biophysica acta. Molecular basis of disease

Wu X,Wang K,Hua W,Li S,Liu X,Liu W,Song Y,Zhang Y,Shao Z,Yang C

Thu Jun 01 00:00:00 EDT 2017

Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways.

Biochimica et biophysica acta. Molecular basis of disease

Wu X,Wang K,Hua W,Li S,Liu X,Liu W,Song Y,Zhang Y,Shao Z,Yang C

Thu Jun 01 00:00:00 EDT 2017

Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways.

Biochimica et biophysica acta. Molecular basis of disease

Wu X,Wang K,Hua W,Li S,Liu X,Liu W,Song Y,Zhang Y,Shao Z,Yang C

Thu Jun 01 00:00:00 EDT 2017

Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways.

Biochimica et biophysica acta. Molecular basis of disease

Wu X,Wang K,Hua W,Li S,Liu X,Liu W,Song Y,Zhang Y,Shao Z,Yang C

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Human) 355

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Function(s)

protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FAS/CD95 Monoclonal Antibody (1G7F7)

FAS/CD95 Antibody (60196-1-IG) in IHC (P)

Product Details

60196-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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