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Invitrogen
FASN Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
FASN Antibody (PA5-22111) in ICC/IF
Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FASN Polyclonal Antibody (Product # PA5-22111) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2... View More
Product Details
PA5-22111
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 401 and 694 of Fatty Acid Synthase (Uniprot ID#P49327)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11154136
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Mouse liver.
Predicted reactivity: Mouse (84%), Rat (85%), Pig (80%), Bovine (81%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I Fatty Acid Synthase
Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1
UniProt ID: (Human) P49327, (Mouse) P19096
Entrez Gene ID: (Human) 2194, (Mouse) 14104
fatty acid synthase activity
[acyl-carrier-protein] S-acetyltransferase activity
[acyl-carrier-protein] S-malonyltransferase activity
3-oxoacyl-[acyl-carrier-protein] synthase activity
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity
oleoyl-[acyl-carrier-protein] hydrolase activity
protein binding
drug binding
myristoyl-[acyl-carrier-protein] hydrolase activity
palmitoyl-[acyl-carrier-protein] hydrolase activity
phosphopantetheine binding
protein homodimerization activity
poly(A) RNA binding
enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
NADPH binding
cadherin binding involved in cell-cell adhesion
3-oxo-pimeloyl-[acp] methyl ester reductase activity
catalytic activity
zinc ion binding
acyl-[acyl-carrier-protein] hydrolase activity
oxidoreductase activity
transferase activity
hydrolase activity
hydrolase activity, acting on ester bonds
lyase activity
3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
identical protein binding
osteoblast differentiation
acetyl-CoA metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
pantothenate metabolic process
mammary gland development
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
oxidation-reduction process
cellular response to interleukin-4
cell-cell adhesion
lipid metabolic process
metabolic process
biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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