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Invitrogen
FASN Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
FASN Antibody (PA5-87440) in IHC (P)
Immunohistochemistry analysis of FASN in paraffin-embedded human gastric cancer. Samples were incubated with FASN Polyclonal antibody (Product # PA5-87440) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-87440
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_0040954)
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804149
Product Specific Information
Immunogen sequence: QQQTQLNLRS LLVNPEGPTL MRLNSVQSSE RPLFLVHPIE GSTTVFHSLA SRLSIPTYGL QCTRAAPLDS IHSLAAYYID CIRQVQPEGP YRVAGYSYGA CVAFEMCSQL QAQQSPAPTH NSLFLFDGSP TYVLAYTQSY RAKLTPGCEA EAETEAICFF VQQFTDMEHN RVLEALLPLK GLEERVAAAV DLIIKSHQGL DRQELSFAAR SFYYKLRAAE QYTPKAKYHG NVMLLRAKTG GAYGEDLGAD YNLSQVCDGK VSVHVIEGDH RTLLEGSGLE SIISIIHSSL AEPRVSVREG; Positive Samples: H460; Cellular Location: Cytoplasm, Melanosome
Target Information
Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I fatty acid synthase
Gene Aliases: FAS; FASN; OA-519; SDR27X1
UniProt ID: (Human) P49327
Entrez Gene ID: (Human) 2194
fatty acid synthase activity
[acyl-carrier-protein] S-acetyltransferase activity
[acyl-carrier-protein] S-malonyltransferase activity
3-oxoacyl-[acyl-carrier-protein] synthase activity
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity
oleoyl-[acyl-carrier-protein] hydrolase activity
protein binding
drug binding
myristoyl-[acyl-carrier-protein] hydrolase activity
palmitoyl-[acyl-carrier-protein] hydrolase activity
phosphopantetheine binding
protein homodimerization activity
poly(A) RNA binding
enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
NADPH binding
cadherin binding involved in cell-cell adhesion
3-oxo-pimeloyl-[acp] methyl ester reductase activity
osteoblast differentiation
acetyl-CoA metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
pantothenate metabolic process
mammary gland development
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
oxidation-reduction process
cellular response to interleukin-4
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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