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Invitrogen
FASN Monoclonal Antibody (C.60.10)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
FASN Antibody (MA5-14887) in ICC/IF
Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More
Product Details
MA5-14887
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
C.60.10
Immunogen
Synthetic peptide around Gly46 corresponding to the sequence of human fatty acid synthase
Conjugate
Form
Liquid
Concentration
5 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10980075
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase
Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1
UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785
Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671
fatty acid synthase activity
[acyl-carrier-protein] S-acetyltransferase activity
[acyl-carrier-protein] S-malonyltransferase activity
3-oxoacyl-[acyl-carrier-protein] synthase activity
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity
oleoyl-[acyl-carrier-protein] hydrolase activity
protein binding
drug binding
myristoyl-[acyl-carrier-protein] hydrolase activity
palmitoyl-[acyl-carrier-protein] hydrolase activity
phosphopantetheine binding
protein homodimerization activity
poly(A) RNA binding
enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
NADPH binding
cadherin binding involved in cell-cell adhesion
3-oxo-pimeloyl-[acp] methyl ester reductase activity
catalytic activity
zinc ion binding
acyl-[acyl-carrier-protein] hydrolase activity
oxidoreductase activity
transferase activity
hydrolase activity
hydrolase activity, acting on ester bonds
lyase activity
3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
identical protein binding
osteoblast differentiation
acetyl-CoA metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
pantothenate metabolic process
mammary gland development
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
oxidation-reduction process
cellular response to interleukin-4
cell-cell adhesion
lipid metabolic process
metabolic process
biosynthetic process
lipid biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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